| biorad t100 thermal cycler manual: Manual of Histological Techniques Santosh Kumar Mondal, 2017-01-31 In this manual, author has included the molecular techniques, immunohistochemistry, cell blocks, and immunofluorescence along with the conventional techniques. For students' easy understanding; many figures, charts, diagrams and tables have been included. At the same time, the volume of the book has been restricted; so that students do not become overburdened during preparation of examination. [Ed.]. |
| biorad t100 thermal cycler manual: Advances in Microfluidics Technology for Diagnostics and Detection David Kinahan, Dario Mager , Elizaveta Vereshchagina, Celina Miyazaki, 2021-09-06 Microfluidics and lab-on-a-chip have, in recent years, come to the forefront in diagnostics and detection. At point-of-care, in the emergency room, and at the hospital bed or GP clinic, lab-on-a-chip offers the potential to rapidly detect time-critical and life-threatening diseases such as sepsis and bacterial meningitis. Furthermore, portable and user-friendly diagnostic platforms can enable disease diagnostics and detection in resource-poor settings where centralised laboratory facilities may not be available. At point-of-use, microfluidics and lab-on-chip can be applied in the field to rapidly identify plant pathogens, thus reducing the need for damaging broad spectrum pesticides while also reducing food losses. Microfluidics can also be applied to the continuous monitoring of water quality and can support policy-makers and protection agencies in protecting the environment. Perhaps most excitingly, microfluidics also offers the potential to enable entirely new diagnostic tests that cannot be implemented using conventional laboratory tools. Examples of microfluidics at the frontier of new medical diagnostic tests include early detection of cancers through circulating tumour cells (CTCs) and highly sensitive genetic tests using droplet-based digital PCR. This Special Issue on “Advances in Microfluidics Technology for Diagnostics and Detection” aims to gather outstanding research and to carry out comprehensive coverage of all aspects related to microfluidics in diagnostics and detection. |
| biorad t100 thermal cycler manual: Genetic and proteomic biomarkers in solid tumor detection and treatment Jitian Li, Apeng Chen, Ruowen Zhang, Changwei Li, Jian-Guo Zhou, 2023-06-07 |
| biorad t100 thermal cycler manual: Characterization of Bioactive Components in Edible Algae Leonel Pereira, 2020-04-15 Algae have been used since ancient times as food for humans, animal feed, agricultural fertilizer, and as a source of substances for therapeutic use. Currently, seaweed represents a vast source of raw materials used in the pharmaceutical, food, traditional medicine, and cosmetics industries. They are nutritionally valuable, both fresh and dried, or as ingredients in a wide variety of pre-made foods. In particular, seaweed contains significant amounts of protein, lipids, minerals, and vitamins. Information is limited on the role of algae and their metabolites in therapy. Only a few taxa have been studied for use in medicine. Many traditional cultures report the healing powers of selected algae in tropical and subtropical marine forms. This is especially true in the maritime areas of Asia, where the sea plays a significant role in daily activities. However, currently, only a few genera and species of algae are involved in aspects of medicine and therapy. The beneficial uses of seaweed or seaweed products include those that can mimic specific manifestations of human disease, production of antibiotic compounds, or improved human nutrition. |
| biorad t100 thermal cycler manual: Science John Michels (Journalist), 2011 |
| biorad t100 thermal cycler manual: Gilles de la Tourette Syndrome: Cross-Cultural Perspectives with a Focus on the Asia-Pacific Region Valsamma Eapen, Yukiko Kano, 2021-06-04 |
| biorad t100 thermal cycler manual: Agriculture and Natural Resources , 2018 |
| biorad t100 thermal cycler manual: Biotechnology J. Kirk Brown, 2011 |
| biorad t100 thermal cycler manual: Lumpy Skin Disease Eeva S. M. Tuppurainen, Shawn Babiuk, Eyal Klement, 2018-07-03 This book provides a comprehensive but concise overview on the economically important emerging cattle pox virus derived Lumpy Skin Disease, including the characteristics of causative agent, description of clinical signs in cattle, pathology and histopathology, immunity, geographical distribution, epidemiology and transmission pathways, control and eradication of the disease. In addition the recent developments in vaccination, mathematical modeling and risk assessment are discussed. Lumpy Skin Disease currently spreads aggressively across the Middle and Near East. The first incursion to the European Union territory occurred in Greece in autumn 2015. The book targets clinicians and field veterinarians in Lumpy Skin Disease affected regions, veterinary authorities as well as advanced students in veterinary medicine and virology. |
| biorad t100 thermal cycler manual: Digital PCR George Karlin-Neumann, Francisco Bizouarn, 2018-05-01 This volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Digital PCR: Methods and Protocols is a valuable resource for specialists in various fields, including geneticists, neurologists, immunologists, oncologists, and researchers who are interested in environmental sciences.” |
| biorad t100 thermal cycler manual: Cell Electrophoresis Johann Bauer, 1994-09-09 This book presents a summary of the application and instrumentation of cell electrophoresis. The method of making cell purification and characterization possible according to the cellular negative surface charge density is discussed, and ideas for future developments are explained. The negative electrostatic forces at cell surfaces provide information about cell-cell interaction, blood vessel sealing, cytokine actions, cell transformation, ion transport phenomena, and other biological phenomena. Recalculations of the physical principles of cell electrophoresis reveal possibilities for removing disruptive factors caused by electrical current, heat, and sedimentation. The introduction of computer technology, the performance of simultaneous two-parameter measurements, and the application of cell-friendly but current-inert buffer systems render the method more reliable and efficient. |
| biorad t100 thermal cycler manual: Diagnostics in Plant Breeding Thomas Lübberstedt, Rajeev Varshney, 2013-02-28 “Diagnostics in Plant Breeding” is systematically organizing cutting-edge research reviews on the development and application of molecular tools for the prediction of plant performance. Given its significance for mankind and the available research resources, medical sciences are leading the area of molecular diagnostics, where DNA-based risk assessments for various diseases and biomarkers to determine their onset become increasingly available. So far, most research in plant genomics has been directed towards understanding the molecular basis of biological processes or phenotypic traits. From a plant breeding perspective, however, the main interest is in predicting optimal genotypes based on molecular information for more time- and cost-efficient breeding schemes. It is anticipated that progress in plant genomics and in particular sequence technology made recently will shift the focus from “explanatory” to “predictive” in crop science. This book assembles chapters on all areas relevant to development and application of predictive molecular tools in plant breeding by leading authorties in the respective areas. |
| biorad t100 thermal cycler manual: Eurasian Arctic Land Cover and Land Use in a Changing Climate Garik Gutman, Anni Reissell, 2010-10-26 This volume is a compilation of studies on interactions of land-cover/land-use change with climate in a region where the climate warming is most pronounced compared to other areas of the globe. The climate warming in the far North, and in the Arctic region of Northern Eurasia in particular, affects both the landscape and human activities, and hence human dimensions are an important aspect of the topic. Environmental pollution together with climate warming may produce irreversible damages to the current Arctic ecosystems. Regional land-atmosphere feedbacks may have large global importance. Remote sensing is a primary tool in studying vast northern territories where in situ observations are sporadic. State-of-the-art methods of satellite remote sensing combined with GIS and models are used to tackle science questions and provide an outlook of current land-cover changes and potential scenarios for the future. Audience: The book is a truly international effort involving U.S. and European scientists. It is directed at the broad science community including graduate students, academics and other professionals in this field. |
| biorad t100 thermal cycler manual: Current Strategies in Cancer Gene Therapy Wolfgang Walther, 2017-01-17 This book describes important developments and emerging trends in experimental and clinical cancer gene therapy. It reflects the tremendous advances made over recent years with respect to immunogenes, suicide genes and gene correction therapies, as well as in gene suppression and miRNA therapies. Many of the described strategies focus on the generation of more efficient and specific means of attack at known and novel cellular targets associated with tumor development and progression. The book also details parallel improvements in vector design, vector delivery, and therapeutic efficacy. It offers readers a stimulating, broad overview of advances in the field, linking experimental strategies to their clinical applications. |
| biorad t100 thermal cycler manual: Veterinary Parasitology M. A. Taylor, R. L. Coop, Richard L. Wall, 2015-12-21 The recipient of much praise and acclaim, Veterinary Parasitology is widely considered to be the definitive veterinary parasitology reference for practitioners and students alike. This Fourth Edition has been developed and enhanced into a two-part reference to reflect recent advances in the field, modern teaching practice, and updated parasite taxonomic classification systems. Part One contains expanded individual parasite descriptions using current taxonomic status within three new chapters on Helminthology, Protozoology and Entomology. Further updated chapters are provided on: The laboratory diagnosis of parasitism, Antiparasitics, The epidemiology of parasitic diseases, and Host resistance to parasitic diseases. Host species chapters have been retained and expanded and are found in Part Two of the edition. KEY FEATURES Tailored for those directly involved in the diagnosis, treatment and control of parasitic diseases of domestic animals Compatible with the diversity of current parasitology teaching modules – both for teaching parasite systematics and diseases on a host-organ basis Offers the most detailed parasite descriptions available today for teachers, research groups, veterinarians in practice and in government service, and others involved in aspects of parasitic disease Thoroughly revised and restructured to reflect the most up-to-date advancements in the field, Veterinary Parasitology, Fourth Edition, enhances its stellar reputation as the gold standard reference text for the global veterinary profession. |
| biorad t100 thermal cycler manual: Phytoplankton Manual Alain Sournia, 1978 |
| biorad t100 thermal cycler manual: Plant Genetics and Molecular Biology Rajeev K. Varshney, Manish K. Pandey, Annapurna Chitikineni, 2018-09-04 This book reviews the latest advances in multiple fields of plant biotechnology and the opportunities that plant genetics, genomics and molecular biology have offered for agriculture improvement. Advanced technologies can dramatically enhance our capacity in understanding the molecular basis of traits and utilizing the available resources for accelerated development of high yielding, nutritious, input-use efficient and climate-smart crop varieties. In this book, readers will discover the significant advances in plant genetics, structural and functional genomics, trait and gene discovery, transcriptomics, proteomics, metabolomics, epigenomics, nanotechnology and analytical & decision support tools in breeding. This book appeals to researchers, academics and other stakeholders of global agriculture. |
| biorad t100 thermal cycler manual: DNA Traffic in the Environment Hiromi Nishida, Taku Oshima, 2019-02-21 This book comprehensively discusses our current understanding of the role and biological mechanisms of horizontal transfer of genetic elements in the environment, which has been important in the evolution of prokaryotes (archaea and bacteria). Horizontal transfer of genetic elements generates variations of prokaryotes and their genomes. Comparative studies of genomes revealed that it frequently occurred during archaeal and bacterial evolution. The book introduces a variety of studies related to horizontal gene transfer, gene silencing, plasmids, phages, transposons, and the emergence of microbes that degrade man-made xenobiotics and have antimicrobial resistance. Written by leading researchers in DNA traffic, the book is a valuable guide to horizontal transfer for both young scientists and experts in the field. |
| biorad t100 thermal cycler manual: Plant Pathology Christophe Lacomme, 2015-05-19 The second edition of Plant Pathology: Techniques and Protocols covers diagnostic methods that are currently used in laboratories for a broad range of plant species and matrixes. These include serological and molecular methods that have one or more of the following characteristics: suitability for high-throughput testing, detection of a group of pathogens or of sometimes uncharacterized pathogens, detection and identification of specific pathogens, and high sensitivity. This volume discusses qualitative and quantitative tests, as well as recently developed diagnostic methods. It also provides background information on many pathogens, which are either endemic, non-endemic, or emerging and with different lifecycles that cause diseases of significant importance in a wide variety of hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, background information on pathogens and the disease caused, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Plant Pathology: Techniques and Protocols, Second Edition is the perfect book for plant pathologists and molecular biologists who will use this information to test out the latest research in their laboratories. |
| biorad t100 thermal cycler manual: Bacterial Chromatin Remus T. Dame, |
| biorad t100 thermal cycler manual: Handbook of Bioseparations Satinder Ahuja, 2000-06-23 It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods, capillary electrophoresis, isoelectric focusing, and mass spectrometry. Among separation and purification methods, liquid-liquid distribution, displacement chromatography, expanded bed adsorption, membrane chromatography, and simulated moving bed chromatography are covered at length. Regulatory and economic considerations are addressed, as are plant and process equipment and engineering process control. A chapter on future developments highlights the application of DNA chip arrays as well as evolving methodologies for a large number of drugs that are under development for treatment of cancer, AIDS, rheumatoid arthritis, and Alzheimer's disease. Handbook of Bioseparations serves as an essential reference and guidebook for separation scientists working in the pharmaceutical and biotechnology industries, academia, and government laboratories.Key Features* Covers bioseparations of proteins, nucleic acids, and monoclonal antibodies* Encompasses both analytical and process-scale methods* Elucidates the importance of engineering process control* Details selection of plant and process equipment* Addresses economic considerations* Discusses future developments |
| biorad t100 thermal cycler manual: Statistical Methods for Physical Science , 1994-12-13 This volume of Methods of Experimental Physics provides an extensive introduction to probability and statistics in many areas of the physical sciences, with an emphasis on the emerging area of spatial statistics. The scope of topics covered is wide-ranging-the text discusses a variety of the most commonly used classical methods and addresses newer methods that are applicable or potentially important. The chapter authors motivate readers with their insightful discussions. - Examines basic probability, including coverage of standard distributions, time series models, and Monte Carlo methods - Describes statistical methods, including basic inference, goodness of fit, maximum likelihood, and least squares - Addresses time series analysis, including filtering and spectral analysis - Includes simulations of physical experiments - Features applications of statistics to atmospheric physics and radio astronomy - Covers the increasingly important area of modern statistical computing |
| biorad t100 thermal cycler manual: Therapeutic Fasting: The Buchinger Amplius Method Francoise Wilhelmi de Toledo, Hubert Hohler, 2011-09-21 Learn the world-famous Buchinger method of therapeutic fasting for long-term health benefits Accepted as a classic cure for many medical and physical issues, as well as a key to good general health, Otto Buchinger's therapeutic fasting methods have attracted millions of followers and been in use for more than five decades. In Therapeutic Fasting, written by an internationally renowned authority on fasting (also, a member of the original Buchinger family), the details and methodology of this highly effective program are disclosed for a whole new generation of readers and clinicians. Special Features Offers easy-to-follow guidelines for implementing the original, acclaimed Buchinger method of therapeutic fasting, which is firmly rooted in concepts of medical sciences and physiology Enhanced by step-by-step instructions, how-to tips, clear explanations, full-color photographs, and compelling personal commentary by individuals who have experienced the program The author Françoise Wilhelmi de Toledo, MD, is an international authority on fasting and heads the well-known Buchinger clinics in Europe. Explores the far-reaching physical, spiritual, and psychological effects of fasting Shows how motivational techniques and physical exercises complement the program and lead to a strong sense of well-being, with examples of yoga, correct breathing, abdominal massage, and more Demonstrates how food is slowly and safely introduced after the fast, with more than 40 savory recipes that promote nutritional awareness in everyday life The only book on this topic available in English, Therapeutic Fasting: The Buchinger-Amplius Method will enable all readers to learn patterns of healthy behavior, practice them with professional guidance, and integrate them into a balanced, healthy lifestyle. It is a fascinating reference for general practitioners, nutritionists, and informed lay people who want to learn more about the powerful combination of therapeutic fasting, supporting scientific medical principles, and natural healing concepts. |
| biorad t100 thermal cycler manual: Olive and Olive Oil Bioactive Constituents Dimitrios Boskou, 2015-08-15 The market is flooded with products posing as elixirs, supplements, functional foods, and olive oil alternatives containing phenols obtained from multiple olive sources. This technically-oriented book will be of value to nutritionists and researchers in the biosciences. It unravels the body of science pertaining to olive minor constituents in relation to new chemical knowledge, technological innovations, and novel methods of recovery, parallel to toxicology, pharmacology, efficacy, doses, claims, and regulation. Topics include: the biological importance of bioactive compounds present in olive products; developments and innovations to preserve the level of bioactives in table olives and olive oil; and importance of variety, maturity, processing of olives, storage, debittering of olives and table olives as a valuable source of bioactive compounds. - Presents detailed information concerning the claimed benefits of olive oil and discusses the permitted health claim to EFSA on oils with natural phenolics - Recovery of bioactive constituents from olive waste is comprehensively described - Explores the relationship betwen phenolic levels and sensory evaluation - Features chapters on the clinical and cellular mechanisms and health effects of olive, important for functional foods research |
| biorad t100 thermal cycler manual: Diseases of Cattle in the Tropics Miodrag Ristic, 2013-03-14 Most of the future increase in livestock production is expected to occur in the tropical and subtropical regions of the world. Cattle are the most numerous of the ruminant species in the tropics and provide the largest quantity of animal food products. More than one-third of the world's cattle are found in the tropics. Disease is the major factor which prohibits full utilization of these regions for cattle production. Various infectious and transmissible viral, rick ettsial, bacterial, and particularly protozoan and helminthic diseases, are widespread in the tropics and exert a heavy toll on the existing cattle industry there. This uncontrolled disease situation also discourages investment in cattle industries by private and government sectors. In Africa alone, it is estimated that 125 million head of cattle could be accommodated in the tropical rainbelt if the disease and other animal husbandry factors could be resolved. The potential of efficient cattle production under more favorable conditions prompted various international agencies to establish a multi million dollar International Laboratory for Research in Animal Diseases (ILRAD) in Nairobi, Kenya, Africa. In South America, principal sites for raising cattle are shifting to the savannah lands because the more fertile soils are being used for crop produc tion, however, in the savannahs also, disease remains the most powerful deterrent in implementing the cattle industry. |
| biorad t100 thermal cycler manual: The Retinoblastoma Protein Pedro G. Santiago-Cardona, 2018-02-22 This volume covers the mechanisms of pRb inactivation detailing repressive mechanisms commonly associated to cancer, and representative of the experimentally relevant tests used in the establishment of cancer diagnosis and prognosis. Chapters contain protocols and in-depth discussions for commonly used experimental approaches to assess the status and function of components of the pRb pathway, including pRb itself, in cell lines and biological samples.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Retinoblastoma Protein aims to serve as a guide to assist molecular cancer biologists in their search for understanding of the molecular functions of this preeminent tumor suppressor. |
| biorad t100 thermal cycler manual: Microtubule Dynamics Anne Straube, 2017-04-30 Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field. |
| biorad t100 thermal cycler manual: Natural Product Biosynthesis Christopher T. Walsh, Yi Tang, 2017-04-28 This textbook describes the types of natural products, the biosynthetic pathways that enable the production of these molecules, and an update on the discovery of novel products in the post-genomic era. |
| biorad t100 thermal cycler manual: Evolution, Origin of Life, Concepts and Methods Pierre Pontarotti, 2020-10-14 This book presents 15 selected contributions to the 22nd Evolutionary Biology Meeting, which took place in September 2018 in Marseille. They are grouped under the following major themes: · Origin of Life · Concepts and Methods · Genome and Phenotype Evolution The aims of these annual meetings in Marseille are to bring together leading evolutionary biologists and other scientists who employ evolutionary biology concepts, e.g. for medical research, and to promote the exchange of ideas and encourage interdisciplinary collaborations. Offering an up-to-date overview of recent advances in the field of evolutionary biology, this book represents an invaluable source of information for scientists, teachers and advanced students. |
| biorad t100 thermal cycler manual: Generalized Linear Mixed Models Walter W. Stroup, 2016-04-19 With numerous examples using SAS PROC GLIMMIX, this text presents an introduction to linear modeling using the generalized linear mixed model as an overarching conceptual framework. For readers new to linear models, the book helps them see the big picture. It shows how linear models fit with the rest of the core statistics curriculum and points out the major issues that statistical modelers must consider. |
| biorad t100 thermal cycler manual: Cancer Immunosurveillance Alejandro López-Soto, Alicia R. Folgueras, 2018-11-22 This volume explores the latest techniques used to study tumor immunology. The chapters in this book detail methodologies for functional analysis and expansion of T lymphocytes for cancer research. The chapters also cover topics such as how single-cell RNA-sequencing can be exploited to dissect immune cell heterogeneity and precursors; isolating and expanding natural killer (NK) cells; evaluating NK cell-mediated anti-tumor killing activity in vitro; immunosurveillance orchestrated by specific immune subsets; use of HLA peptidomics for cancer-exome based identification of tumor neo-antigens; gold standard assessment of immunogenic cell death in oncological mouse models and methods to look at the therapeutic relevance of immune modulation in cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cancer Immunosurveillance: Methods and Protocols is a valuable resource to aid researchers in better understanding and experimenting in this exciting and developing field. |
| biorad t100 thermal cycler manual: African Antelope Database 1998 Rod East, 1999 Although most antelope species still exist in large numbers in sub-Saharan Africa (some in hundreds of thousands), up to three-quarters of the species are in decline. Threats to their survival arise from the rapid growth of human and livestock populations, with consequent degradation and destruction of natural habitats, and excessive offtake by meat hunters. In addition, some parts of Africa are mow almost completely devoid of large wild animals because of uncontrolled slaughter during recent civil wars. This report presents the information currently held by the IUCN/SSC Antelope Specialist Group on the conservation status of each antelope species (and selected subspecies) in sub-Saharan Africa. Key areas have been identified for the conservation of representative antelope communities. While external donors make the greatest contributions to the conservation of antelopes, greater recognition of wildlife conservation in national and regional development plans is often a critically important requirement. |
| biorad t100 thermal cycler manual: Melanoma Kristian M. Hargadon, 2022-03-26 This extensive book brings together leading melanoma researchers from across the world and highlights many of the cutting-edge protocols and experimental systems currently being used to investigate questions surrounding this disease. The volume opens with sections on 2D and 3D cell culture-based approaches for studying melanoma biology, and continues with collections of chapters examining various approaches for detecting, isolating, and characterizing circulating melanoma cells, circulating tumor DNA, and exosomes, as well as experimental procedures for studying and detecting melanoma metastasis in both pre-clinical and clinical settings, bioinformatics-based approaches, protocols for quantifying and characterizing immune cell infiltrates in both melanoma tumors and tertiary lymphoid structures, and development and evaluation of therapeutic strategies for melanoma treatment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Melanoma: Methods and Protocols aims to serve basic research scientists and clinicians who bring questions from the clinic into the lab in order to translate observations in the laboratory into improved patient care for this highly malignant form of cancer. Chapter 14 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. |
| biorad t100 thermal cycler manual: Hospital Wastewaters Paola Verlicchi, 2017-09-04 This volume addresses hospital effluents in terms of their composition and the management and treatment strategies currently (being) adopted around the globe. In this context, one major focus is on pharmaceutical compounds: their observed concentration range, ecotoxicological effects, and the removal efficiency achieved by the different technologies. Another focus is on management strategies (dedicated hospital wastewater treatment, or a combined approach also involving urban wastewater) and currently adopted treatments to reduce the released pollutant load. Innovative and promising technologies under investigation at the lab and pilot scale are presented. A discussion of remaining knowledge gaps and future research requirements rounds out the coverage. The respective chapters, written by experts in the different fields, provide useful information for a broad audience: scientists involved in the management and treatment of hospital effluents and wastewater containing micropollutants, administrators and decision-makers, legislators involved in the authorization and management of healthcare structure effluents, and environmental engineers involved in the design of wastewater treatment plants, as well as newcomers and students interested in these issues. |
| biorad t100 thermal cycler manual: Physiology of Fish in Intensive Culture Systems Gary A. Wedemeyer, 2012-12-06 Fish culture in hatcheries and other aquacultural facilities is becoming much more intensive all over the world. The success of all kinds of fish rearing depends on the quality of management and this depends, in turn, on understanding the biology of fishes and the aquatic environment in which they live. This book directly addresses the relationship between the aquatic environment and the fishes. An understanding of this by the reader will result in a reduction of disease outbreaks through improved management. |
| biorad t100 thermal cycler manual: Applied Plant Genomics and Biotechnology Palmiro Poltronieri, Yiguo Hong, 2015-01-27 Applied plant genomics and biotechnology reviews the recent advancements in the post-genomic era, discussing how different varieties respond to abiotic and biotic stresses, investigating epigenetic modifications and epigenetic memory through analysis of DNA methylation states, applicative uses of RNA silencing and RNA interference in plant physiology and in experimental transgenics, and plants modified to produce high-value pharmaceutical proteins. The book provides an overview of research advances in application of RNA silencing and RNA interference, through Virus-based transient gene expression systems, Virus induced gene complementation (VIGC), Virus induced gene silencing (Sir VIGS, Mr VIGS) Virus-based microRNA silencing (VbMS) and Virus-based RNA mobility assays (VRMA); RNA based vaccines and expression of virus proteins or RNA, and virus-like particles in plants, the potential of virus vaccines and therapeutics, and exploring plants as factories for useful products and pharmaceuticals are topics wholly deepened. The book reviews and discuss Plant Functional Genomic studies discussing the technologies supporting the genetic improvement of plants and the production of plant varieties more resistant to biotic and abiotic stresses. Several important crops are analysed providing a glimpse on the most up-to-date methods and topics of investigation. The book presents a review on current state of GMO, the cisgenesis-derived plants and novel plant products devoid of transgene elements, discuss their regulation and the production of desired traits such as resistance to viruses and disease also in fruit trees and wood trees with long vegetative periods. Several chapters cover aspects of plant physiology related to plant improvement: cytokinin metabolism and hormone signaling pathways are discussed in barley; PARP-domain proteins involved in Stress-Induced Morphogenetic Response, regulation of NAD signaling and ROS dependent synthesis of anthocyanins. Apple allergen isoforms and the various content in different varieties are discussed and approaches to reduce their presence. Euphorbiaceae, castor bean, cassava and Jathropa are discussed at genomic structure, their diseases and viruses, and methods of transformation. Rice genomics and agricultural traits are discussed, and biotechnology for engineering and improve rice varieties. Mango topics are presented with an overview of molecular methods for variety differentiation, and aspects of fruit improvement by traditional and biotechnology methods. Oilseed rape is presented, discussing the genetic diversity, quality traits, genetic maps, genomic selection and comparative genomics for improvement of varieties. Tomato studies are presented, with an overview on the knowledge of the regulatory networks involved in flowering, methods applied to study the tomato genome-wide DNA methylation, its regulation by small RNAs, microRNA-dependent control of transcription factors expression, the development and ripening processes in tomato, genomic studies and fruit modelling to establish fleshy fruit traits of interest; the gene reprogramming during fruit ripening, and the ethylene dependent and independent DNA methylation changes. - provides an overview on the ongoing projects and activities in the field of applied biotechnology - includes examples of different crops and applications to be exploited - reviews and discusses Plant Functional Genomic studies and the future developments in the field - explores the new technologies supporting the genetic improvement of plants |
| biorad t100 thermal cycler manual: Green Science and Technology Enoch Y. Park, Takayuki Saito, Hirokazu Kawagishi, Masakazu Hara, 2019-10-16 This book provides a comprehensive and up-to-date review of recent trends of green science and technology. Worldwide deterioration of environment and global warming threaten our lifestyle and the survival of all creatures. In order to weather these problems, we need to construct a multidisciplinary approach involving the fusion of various advanced researches. The book begins with an overview on fundamental research about generation and utilization of renewable energy, protection of the earth's ecosystem for better coexistence with nature, development of artificial intelligence-based agriculture and molecular recognitionbased welfare and covers a wide range of innovative research on green science and technology. |
| biorad t100 thermal cycler manual: Molecular Life Sciences Robert D. Wells, Judith S. Bond, Judith Klinman, Bettie Sue Siler Masters, Ellis Bell, Laurie S. Kaguni, 2018-01-12 Molecular Life Sciences: An Encyclopedic Reference will focus on understanding biological phenomena at the level of molecules and their interactions that govern life processes. The work will include articles on genes and genomes, protein structure and function, systems biology using genomics and proteomics as the focus, molecular aspects of cell structure and function, unifying concepts and theories from biology, chemistry, mathematics and physics that are essential for understanding the molecular life sciences (including teaching perspectives and assessment tools), and basic aspects of the various experimental approaches that are used in the Molecular Life Sciences. |
| biorad t100 thermal cycler manual: Elucidation of Abiotic Stress Signaling in Plants Girdhar K. Pandey, 2015-05-30 Abiotic stresses such as high temperature, low-temperature, drought, and salinity limit crop productivity worldwide. Understanding plant responses to these stresses is essential for rational engineering of crop plants. In Arabidopsis, the signal transduction pathways for abiotic stresses, light, several phytohormones and pathogenesis have been elucidated. A significant portion of plant genomes (most studies are Arabidopsis and rice genome) encodes for proteins involves in signaling such as receptor, sensors, kinases, phosphatases, transcription factors and transporters/channels. Despite decades of physiological and molecular effort, knowledge pertaining to how plants sense and transduce low and high temperature, low-water availability (drought), water-submergence and salinity signals is still a major question before plant biologists. One major constraint hampering our understanding of these signal transduction processes in plants has been the lack or slow pace of application of molecular genomic and genetics knowledge in the form of gene function. In the post-genomic era, one of the major challenges is investigation and understanding of multiple genes and gene families regulating a particular physiological and developmental aspect of plant life cycle. One of the important physiological processes is regulation of stress response, which leads to adaptation or adjustment in response to adverse stimuli. With the holistic understanding of the signaling pathways involving not only one gene family but multiple genes or gene families, plant biologists can lay a foundation for designing and generating future crops that can withstand the higher degree of environmental stresses (especially abiotic stresses, which are the major cause of crop loss throughout the world) without losing crop yield and productivity. |
| biorad t100 thermal cycler manual: Genomics, Circuits, and Pathways in Clinical Neuropsychiatry Thomas Lehner, Bruce L. Miller, Matthew W. State, 2016-06-07 This foundational work comprehensively examines the current state of the genetics, genomics and brain circuitry of psychiatric and neurological disorders. It consolidates discoveries of specific genes and genomic regions associated with these conditions, the genetic and anatomic architecture of these syndromes, and addresses how recent advances in genomics are leading to a reappraisal of the biology underlying clinical neuroscience. In doing so, it critically examines the promise and limitations of these discoveries toward treatment, and to the interdisciplinary nature of understanding brain and behavior. Coverage includes new discoveries regarding autism, epilepsy, intellectual disability, dementias, movement disorders, language impairment, disorders of attention, schizophrenia, and bipolar disorder. Genomics, Circuits, and Pathways in Clinical Neuropsychiatry focuses on key concepts, challenges, findings, and methods in genetics, genomics, molecular pathways, brain circuitry, and related neurobiology of neurologic and psychiatric disorders. - Provides interdisciplinary appeal in psychiatry, neurology, neuroscience, and genetics - Identifies key concepts, methods, and findings - Includes coverage of multiple disorders from autism to schizophrenia - Reviews specific genes associated with disorders - Discusses the genetic architecture of these syndromes - Explains how recent findings are influencing the understanding of biology - Clarifies the promise of these findings for future treatment |
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