The Quick Answer
No, chapter titles are generally not italicized. In Chicago Manual of Style, MLA, APA, and most major style guides, chapter titles appear in regular Roman type with title-case capitalization. Italics are reserved for the book title itself or individual shorter works mentioned within the text. This is consistent across fiction and nonfiction publishing. When you are looking at Are Chapter Titles Italicized, the answer depends a bit on which style guide you are following, but nearly all of them say the same thing. A chapter heading sits on its own line, centered or left-aligned depending on the publisher's preference, in plain blackface or whatever font the interior uses. Below it comes the actual chapter number or name, also in plain type. The page that follows contains the body text. Nothing fancy. Nothing italicized. I run into this question constantly from self-publishing authors and students working on theses. They see a book cover with stylized chapter names in italics and assume that carries through to the interior. It does not. The cover is a design decision. The interior follows the style guide.
Are Chapter Titles Italicized
Here is where people get tripped up. Chapter titles themselves are plain, but there are a handful of edge cases where italics appear nearby and the distinction gets blurry. Subtitle after a chapter title: If your chapter heading reads "Chapter 3: The Fall of Rome," the subtitle part stays in regular type. Only if you are citing a work within your text would that phrase potentially take italics, and even then it depends on context. Foreign language chapter titles: Some authors write certain chapters in another language. Chicago says to keep those in regular type unless the language demands otherwise. Italics do not replace proper transliteration rules.
Manuscript submissions vs. published books: This is the one I mess up most when I am advising people. In manuscript format, you center the chapter title on the page and do not bold or italicize it. Simple. Once the book goes to production, the designer may apply all-caps, small caps, or a different font weight, but still no italics. I once had an author submit a manuscript where the chapter names were already italicized because they had copy-pasted them from a Word template that auto-formatted headings. We spent two days stripping the italics before it could go to typesetting. Do not skip the manual clean-up pass.
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The Counter-Intuitive Part Most People Miss
Everyone assumes the rule is uniform, but it is not. Some academic publishers and rare manuscript traditions do use italics for chapter titles. This happens most often in older scholarly editions of primary sources or in certain humanities dissertations. If your institution or publisher has a specific style sheet, check it first. The default answer of "no italics" is wrong only when a particular house style overrides it. Another thing nobody warns you about: when a chapter title includes a title of another work inside it, that embedded title gets italicized. Example: "Chapter 7: Understanding Orwell's 1984." The chapter name is plain, but the book title inside it is italicized because it is a standalone work. This is a common formatting error that copy editors catch late in the process and it adds unnecessary revision cycles.
What Each Major Style Guide Says
Chicago Manual of Style (17th ed.): Chapter headings are set in roman type. Section numbers and titles appear in the same font as the surrounding text. The book title in your front matter may be italicized, but the chapter name is not. See CMOS 8.16 through 8.24 for manuscript formatting. MLA (9th ed.): MLA does not prescribe book interior design. It governs citations and works-cited entries. When MLA-formatting a chapter within a book in your bibliography, the chapter title goes in quotation marks, not in italics. The book title is italicized. This distinction matters for paper submissions and academic references. APA (7th ed.): APA is primarily for social sciences and journal articles. For books, chapter titles in reference list entries use sentence case and quotation marks. Chapter headings within the manuscript itself follow standard formatting conventions. Italics are not applied to chapter titles.
The Bluebook and legal citation: Legal briefs sometimes use different conventions for section headings. Chapter-style headings in legal documents are typically bolded, not italicized. This is worth noting if you are working in law review format.

A Practical Workflow That Saves Time
Here is how I handle chapter title formatting in a real project. I set up a custom Word style called "Chapter Title" with these properties: centered alignment, 14-point font matching the body, no italics, no bold, single spacing above and below, and the paragraph before set to auto-break so each chapter starts on a new page. This takes about ten minutes to configure. Once it is done, every chapter I add applies the style automatically, which eliminates inconsistent formatting during the draft phase. Before sending to a typesetter or submitting, I do a find-and-replace scan for any italicized text in the heading styles. Sometimes my own pasting habits sneak italics in. I also run a second check for quotation marks around chapter titles, which should never appear in the interior text, only in the bibliography.
When This Rule Completely Fails
The "no italics" rule breaks down in a few specific scenarios that most guides do not mention clearly. Poetry collections and experimental fiction: Some contemporary literary publishers intentionally italicize chapter titles as a stylistic choice. This is a design decision, not a style guide rule. If you are writing a novel with unconventional formatting, your publisher may request italics. Assume standard rules apply until your editor tells you otherwise. Thesis and dissertation formatting: University graduate schools sometimes have their own requirement that differs from Chicago or MLA. A few institutions require chapter titles in italics for the hardcopy submission. I have seen this at two universities in the last three years. Always check the graduate school formatting guidelines before you spend hours setting up styles that will need to be redone.
Self-publishing platforms: Amazon KDP and similar services do not enforce a style guide. Your formatter decides. Many indie authors italicize chapter titles because they think it looks better. It is legally and technically fine to do so. The rule against italics exists in professional publishing, not in self-publishing. Know the difference and decide accordingly.

Summary Without the Summary
Chapter titles are plain text in standard publishing. Chicago, MLA, and APA all agree on this. The exceptions are niche, situational, and usually come from a specific publisher or institution rather than from the style guides themselves. Set up your document styles correctly at the beginning, verify your target style guide or publisher requirements, and do a final formatting sweep before submission. This approach typically prevents at least one round of unnecessary revisions.