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| computer-assisted sperm analysis pdf: Manual of Sperm Function Testing in Human Assisted Reproduction Ashok Agarwal, Ralf Henkel, Ahmad Majzoub, 2021-04-15 Focusing on modern sperm function testing, this guide is essential in selecting sperm that will produce viable and healthy embryos. |
| computer-assisted sperm analysis pdf: Equine Reproductive Procedures John Dascanio, Patrick McCue, 2014-06-23 Equine Reproductive Procedures is a user-friendly guide to reproductive management, diagnostic techniques, and therapeutic techniques on stallions, mares, and foals. Offering detailed descriptions of 161 procedures ranging from common to highly specialized, the book gives step-by-step instructions with interpretative information, as well as useful equipment lists and references for further reading. Presented in a highly portable spiral-bound format, Equine Reproductive Procedures is a practical resource for daily use in equine practice. Divided into sections on the non-pregnant mare, the pregnant mare, the postpartum mare, the stallion, and the newborn foal, the book is well-illustrated throughout with clinical photographs demonstrating procedures. Equine Reproductive Procedures provides practical guidance for performing basic and advanced techniques associated with the medical management of horses. |
| computer-assisted sperm analysis pdf: WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction World Health Organisation, 1999-05-13 The definitive and essential source of reference for all laboratories involved in the analysis of human semen. |
| computer-assisted sperm analysis pdf: A Practical Guide to Basic Laboratory Andrology Lars Björndahl, David Mortimer, Christopher L. R. Barratt, Jose Antonio Castilla, Roelof Menkveld, Ulrik Kvist, Juan G. Alvarez, Trine B. Haugen, 2010-04-01 This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes pertinent quality control considerations, as well as the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. Throughout the handbook the interpretation of laboratory results in the clinical context is highlighted, and safe laboratory practice is emphasized. It is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility. |
| computer-assisted sperm analysis pdf: Andrological Evaluation of Male Infertility Ashok Agarwal, Sajal Gupta, Rakesh Sharma, 2016-07-26 This state-of-the-art laboratory manual includes 20 clinical protocols used daily for the investigation of the infertile male, presented with easy to understand, step-by-step methodology. The protocols are arranged from routine to advanced laboratory procedures common to clinical practice, including computer-assisted semen analysis, sperm preparation for IUI by density gradient and swim-up, sperm cryopreservation, and sperm DNA fragmentation test by TUNEL method, among others. The methodology in each protocol follows best practice guidelines made clearer by professionally hand-drawn illustrations covering most of the important steps and equipment. The authors, hailing from the world-renowned Andrology Center at Cleveland Clinic, have over 50 years of combined first-hand experience in managing very busy diagnostic and research facilities in male infertility and andrology. The book will be an indispensable resource for thousands of laboratory technologists, clinicians and reproductive professionals (andrologists, embryologist, etc.) engaged in the diagnosis and management of infertile men around the world. |
| computer-assisted sperm analysis pdf: The Microscope in the Dutch Republic Edward G. Ruestow, 1996 Focusing on the two seventeenth-century pioneers of microscopic dicovery, the Dutchmen Jan Swammerdam and Antoni van Leewenhoek, Ruestow demonstrates that their uneasiness with their social circumstances spurred their discoveries. Though arguing that aspects of Dutch culture impeded serious research with the microscope, Ruestow also shows, however, that the culture of the period shaped how Swammerdam and Leewenhoek responded to what they saw through the lens. He concludes by emphasising how their early microscopic efforts differed from the institutionalised microscopic research that began in the nineteenth century. |
| computer-assisted sperm analysis pdf: Practical Laboratory Andrology David Mortimer, 1994 This practical manual on sperm analysis presents the diagnostic and therapeutic procedures that are used in andrology laboratories to analyze and assess male infertility. Diagnostic areas include: semen analysis and the biochemical, immunological and microbiological examination of human semen and spermatozoa; computer-aided sperm motility analysis; sperm ultrastructure; and assessment of sperm transport through the female tract and sperm fertilizing ability. The clinical relevance of various diagnostic procedures is also discussed. Therapeutic topics include sperm washing techniques, semen cryopreservation, and insemination procedures. The volume also covers safety in the andrology laboratory, technician training, and quality control. The text is extensively illustrated and will be an invaluable resource to all scientists and technicians who diagnose male infertility. It will also be of interest to researchers working in human gamete biology and reproductive physiology. The detailed methods described in the book are relevant to all hospital, commercial, and university laboratories involved in infertility diagnosis and treatment. |
| computer-assisted sperm analysis pdf: XIIIth International Symposium on Spermatology Lars Björndahl, John Flanagan, Rebecka Holmberg, Ulrik Kvist, 2021-07-22 These proceedings of the 2018 XIII International Symposium on Spermatology focus on comparative biology, and encourages discussion and the exchange of ideas. The aim of this Symposium was to provide a unique opportunity and bring together scientists from a wide spectrum of research fields – human, domestic animals and other mammals, vertebrates, insects, and plants. The underlying focus is on the function of the spermatozoon – a common feature for sexual reproduction, but extremely varied. By exploring the variability, a better understanding of male reproductive functions can develop. These proceedings address the mechanisms of physiology and pathophysiology, rather than diagnosis and treatment. The symposium featured keynote lectures by invited speakers, followed by presentations on specific aspects of the general topic of the session. Experimental studies are given priority over clinical studies of patient populations. The proceedings comprise both keynote speakers' texts and selected free communications. Posters were considered for publication in the proceedings, and the volume includes exhibited materials on the work of prominent spermatologists, highlighting their important past achievements in the field. |
| computer-assisted sperm analysis pdf: Current Frontiers in Cryobiology Igor Katkov, 2012-03-09 Almost a decade has passed since the last textbook on the science of cryobiology, Life in the Frozen State, was published. Recently, there have been some serious tectonic shifts in cryobiology which were perhaps not seen on the surface but will have a profound effect on both the future of cryobiology and the development of new cryopreservation methods. We feel that it is time to revise the previous paradigms and dogmas, discuss the conceptually new cryobiological ideas, and introduce the recently emerged practical protocols for cryopreservation. The present books, Current Frontiers in Cryobiology and Current Frontiers in Cryopreservation will serve the purpose. This is a global effort by scientists from 27 countries from all continents and we hope it will be interesting to a wide audience. |
| computer-assisted sperm analysis pdf: Animal Andrology Peter J Chenoweth, Steven Lorton, 2014-04-30 Understanding animal andrology is fundamental to optimising genetic breeding traits in domestic and wild animals. This book provides extensive coverage of male reproductive biology, discussing the essentials of sperm production, harvest and preservation before covering the applications to a range of animals including cattle, horses, pigs, small ruminants, camelids, cats and dogs, poultry and exotic species. It also examines the laboratory procedures that provide the basis of general fertility research. |
| computer-assisted sperm analysis pdf: Manual of Intrauterine Insemination and Ovulation Induction Richard P. Dickey, Peter R. Brinsden, Roman Pyrzak, 2009-10-08 A comprehensive and practical account of how to set up and run a successful IUI and ovulation induction program. |
| computer-assisted sperm analysis pdf: Infertility in the Male Larry I. Lipshultz, Stuart S. Howards, Craig S. Niederberger, 2009-09-24 The new edition of this canonical text on male reproductive medicine will cement the book's market-leading position. Practitioners across many specialties - including urologists, gynecologists, reproductive endocrinologists, medical endocrinologists and many in internal medicine and family practice – will see men with suboptimal fertility and reproductive problems. The book provides an excellent source of timely, well-considered information for those training in this young and rapidly evolving field. While several recent books provide targeted 'cookbooks' for those in a male reproductive laboratory, or quick reference for practising generalists, the modern, comprehensive reference providing both a background for male reproductive medicine as well as clinical practice information based on that foundation has been lacking until now. The book has been extensively revised with a particular focus on modern molecular medicine. Appropriate therapeutic interventions are highlighted throughout. |
| computer-assisted sperm analysis pdf: Subfertility, Reproductive Endocrinology and Assisted Reproduction Jane A. Stewart, 2019-06-20 Reproductive medicine and surgery is a major subspecialty in the practice of obstetrics and gynaecology. This textbook provides an introduction to the field of subfertility and reproductive endocrinology, with contributions written by leading experts in the field. This book is based on and compliments a training programme run jointly by the British Fertility Society and the Royal College of Obstetricians and Gynaecologists, making it an indispensable handbook for medical trainees. It will also appeal to clinicians embarking on a career in obstetrics and gynaecology who want an excellent grounding in this area. Specialist nurses will also find the book a valuable resource, and scientists specialising in reproductive medicine and embryology will gain insight into the related clinical areas. |
| computer-assisted sperm analysis pdf: Endocrine Disruption in Fish David E. Kime, 1998-10-31 The last half-century has shown a dramatic increase in the standard of living of millions of people in Europe, North America and many parts of the Third World. This has, in many ways been brought about by scientific and technical developments which were initiated in the 1940s and 1950s. Promises were then made that nuclear energy would provide electricity so cheap that it would not need metering, pesticides would end malnutrition throughout the world and plastics and other synthetic chemicals would revolutionise our manufacturing industry and our way of life. Whilst some of these promises have been fulfilled, the problems of long-term health risks to humans and wildlife arising from the use, production and disposal of these products were either unknown or deliberately understated. Nuclear power is rendered economically unviable when the real cost of decommissioning and storage of waste for several millenia is included, and the effects on health of both humans and wildlife of early pest eradication programmes with organochlorine pesticides were well documented in Rachel Carson's Silent Spring. Evidence of the effects of aerosols and refrigerants on depletion of the ozone layer has led to restriction on the use of CFCs, and there is now increasing evidence of climate change resulting from our profligate use of fossil fuels. |
| computer-assisted sperm analysis pdf: Male Infertility T.B. Hargreave, 2012-12-06 Male infertility is a clinician-orientied book aimed at the clinician dealing with the infertile couple because rational, effective management is only possible if the couple are considered together. The aim of the work is to provide advice to the clinician and to give reference to the underlying science. This will not only enable clinicians to understand the underlying science but will also give scientists an insight to clinical work. This blend of science and clinical work is reflected in the contributors who are experts drawn from both fields. |
| computer-assisted sperm analysis pdf: An Introduction to Male Reproductive Medicine Craig Niederberger, 2011-03-24 An Introduction to Male Reproductive Medicine is written specifically for readers seeking entry into this fast-moving, complex specialty with a solid understanding of the subject. The first chapters cover the anatomy and physiology, clinical evaluation, surgery, medicine, genetics and laboratory testing involved in the current evaluation and treatment of the infertile male, and the final chapter describes the interaction of the field with female reproductive medicine. Throughout the book, references are directly made to the fourth edition of the major text in the specialty, Infertility in the Male, edited by Larry Lipshultz, Stuart Howards and Craig Niederberger, allowing readers to expand their understanding of specific areas where desired. Each chapter is written by a well-renowned expert in an easy to follow, informal style, making the text ideal for students, residents and general physicians who are seeking to increase their general knowledge of the field. |
| computer-assisted sperm analysis pdf: Biological Variation Callum G. Fraser, 2001 |
| computer-assisted sperm analysis pdf: Fertility Preservation in Male Cancer Patients John P. Mulhall, 2013-02-21 Written by international authorities, this book is aimed at clinicians dealing with male patients rendered infertile by cancer therapy. |
| computer-assisted sperm analysis pdf: Textbook of Clinical Embryology Kevin Coward, Dagan Wells, 2013-10-31 The success of Assisted Reproductive Technology is critically dependent upon the use of well optimized protocols, based upon sound scientific reasoning, empirical observations and evidence of clinical efficacy. Recently, the treatment of infertility has experienced a revolution, with the routine adoption of increasingly specialized molecular biological techniques and advanced methods for the manipulation of gametes and embryos. This textbook – inspired by the postgraduate degree program at the University of Oxford – guides students through the multidisciplinary syllabus essential to ART laboratory practice, from basic culture techniques and micromanipulation to laboratory management and quality assurance, and from endocrinology to molecular biology and research methods. Written for all levels of IVF practitioners, reproductive biologists and technologists involved in human reproductive science, it can be used as a reference manual for all IVF labs and as a textbook by undergraduates, advanced students, scientists and professionals involved in gamete, embryo or stem cell biology. |
| computer-assisted sperm analysis pdf: Handbook of the Laboratory Diagnosis and Treatment of Infertility Brooks A. Keel, Bobby W. Webster, 1990-06-22 This book provides comprehensive, detailed, and step-by-step descriptions of materials and methods currently used in laboratory procedures within the infertility clinic. It addresses the numerous highly sophisticated procedures resulting from research in the area of laboratory diagnosis and treatment of infertility. Among those procedures covered are: |
| computer-assisted sperm analysis pdf: Veterinary Cytology Leslie C. Sharkey, M. Judith Radin, Davis M. Seelig, 2020-08-04 This book offers a state-of-the-art, evidence-based reference to all aspects of veterinary cytology. Truly multidisciplinary in its approach, chapters are written by experts in fields ranging from clinical pathology to internal medicine, surgery, ophthalmology, and dermatology, drawing the various specialties together to create a comprehensive picture of cytology's role in diagnosis and treatment of animal disease. Firmly grounded in the primary literature, the book focuses on companion animals, with special chapters for species with fewer publications. Chapters are logically organized by body system, with additional chapters on tumors of particular import and diagnostic decision making. The first two sections of Veterinary Cytology focus on cytology techniques, quality control, and special laboratory techniques. Subsequent sections are organ/tissue-based and reflect what is known about the canine, feline, and equine species. This is followed by chapters on non-traditional species, including exotic companion mammals, rabbits, cattle, camelids, non-human primates, reptiles and birds, amphibians, fish, invertebrates, and sheep and goats. The last section highlights some unique features of the applications of cytology in industry settings. Provides a gold-standard reference to data-driven information about cytologic analysis in companion animal species Brings together authors from a wide range of specialties to present a thorough survey of cytology's use in veterinary medicine Offers broader species coverage and greater depth than any cytology reference currently available Veterinary Cytology is an essential resource for clinical and anatomic pathologists and any specialist in areas using cytology, including veterinary oncologists, criticalists, surgeons, ophthalmologists, dermatologists, and internists. |
| computer-assisted sperm analysis pdf: Equine Hematology, Cytology, and Clinical Chemistry Raquel M. Walton, Rick L. Cowell, Amy C. Valenciano, 2020-11-04 The all-new Equine Hematology, Cytology, and Clinical Chemistry draws on hematology and clinical chemistry information featured in the first edition of Equine Clinical Pathology and adds valuable cytopathology material from Diagnostic Cytology and Hematology of the Horse, making it a truly definitive reference to clinical pathology in equids. Thoroughly updated and expanded throughout, this Second Edition offers more images, more information, and new knowledge for previous chapters and entirely new chapters on bone marrow evaluation and cytopathology. Designed to present clear, concise, and clinically relevant information, the book is logically organized for easy reference. Numerous figures, tables and images support the text, together with summarized information for ease of use. Offers a focus on clinical pathology in the horse, with in-depth information on hematology, clinical chemistry, and cytopathology in equids Presents equine disease from a systems-based, clinicopathological perspective Features hundreds of high-quality images Includes contributions from veterinary specialists with expert knowledge of clinical pathology A must-have purchase for anyone using hematology, clinical chemistry, and cytology in equine patients, Equine Hematology, Cytology, and Clinical Chemistry, 2nd Edition is a valuable resource for equine practitioners, clinical pathologists and residents, and veterinary students. |
| computer-assisted sperm analysis pdf: Methods in Reproductive Aquaculture Elsa Cabrita, Vanesa Robles, Paz Herraez, 2008-08-22 The large amount of information on fish reproduction available is not always readily accessible to all interested parties. Written to appeal to aquaculturalists, conservation managers, and scientific researchers, Methods in Reproductive Aquaculture provides an overview of available techniques and addresses ways to improve depleted stocks of endange |
| computer-assisted sperm analysis pdf: World Wide Research William H. Dutton, Paul W. Jeffreys, 2010-05-21 Experts examine ways in which the use of increasingly powerful and versatile digital information and communication technologies are transforming research activities across all disciplines. Advances in information and communication technology are transforming the way scholarly research is conducted across all disciplines. The use of increasingly powerful and versatile computer-based and networked systems promises to change research activity as profoundly as the mobile phone, the Internet, and email have changed everyday life. This book offers a comprehensive and accessible view of the use of these new approaches—called “e-Research”—and their ethical, legal, and institutional implications. The contributors, leading scholars from a range of disciplines, focus on how e-Research is reshaping not only how research is done but also, and more important, its outcomes. By anchoring their discussion in specific examples and case studies, they identify and analyze a promising set of practical developments and results associated with e-Research innovations. The contributors, who include Geoffrey Bowker, Christine Borgman, Paul Edwards, Tim Berners-Lee, and Hal Abelson, explain why and how e-Research activity can reconfigure access to networks of information, expertise, and experience, changing what researchers observe, with whom they collaborate, how they share information, what methods they use to report their findings, and what knowledge is required to do this. They discuss both the means of e-Research (new research-centered computational networks) and its purpose (to improve the quality of world-wide research). |
| computer-assisted sperm analysis pdf: Spermatology Gerd Ludwig, Julian Frick, 2012-12-06 The analysis of ejaculate, known as the spermiogram, is the crucial investigative technique in the andrological check-up for childlessness. Althoug other impor tant factors such as medical history, physical examination, hormone analysis and tests, biochemical, microbiological, immunological, and genetic tests, and histological and histochemical investigations must all be considered before a final assessment can be made of male fertility, the spermiogram is the guiding factor in estimating the prospects of fertility. Furthermore, the improvement in insemination techniques in recent years and the development of in vitro fertilization have enhanced the importance of the accurate analysis of sperm morphology and function. In view of the multiplicity of andrological investigative techniques listed above, urologists, gynecologists, physicians specializing in endocrinology and immunology, general practitioners, pathologists, and dermatologists are routin ely involved in semen analysis today. We hope in this book to address people working in all these different medical specialities. Having regard for the requirements of daily practice, we will intentionally restrict ourselves to the spermiogram, which we hope to present step by step by means of a large number of illustrations. A substantial part of the book is devoted to the morphological assessment of normal and pathological forms of spermatozoa and other cellular elements, in the form of generously illustrat ed atlas. Dr. Rovan has given us outstanding assistance in preparing the majori ty of photomicrographs. |
| computer-assisted sperm analysis pdf: Sperm Chromatin Armand Zini, Ashok Agarwal, 2011-08-04 Sperm DNA damage is common and has been associated with reduced rates of conception, impaired embryonic development and increased risk of miscarriage. Although the exact causes of sperm DNA damage are unknown, it is clear that infertile men possess substantially higher levels of sperm DNA damage than do fertile men. Written by leading, internationally renowned clinicians and basic scientists with expertise in sperm DNA, Sperm Chromatin: Biological and Clinical Applications in Male Infertility and Assisted Reproduction provides readers with a thoughtful and comprehensive review of the biological and clinical significance of sperm DNA damage. The work covers the fundamental principles of sperm chromatin architecture and function, the proposed modes of DNA damage and repair, the tests of sperm DNA damage, the clinical aspects of DNA damage and the impact of DNA damage on reproductive outcome. Unlike any other title on the topic, Sperm Chromatin: Biological and Clinical Applications in Male Infertility and Assisted Reproduction is an invaluable addition to the literature and will serve as an indispensable resource for basic scientists with an interest in sperm biology and for urologists, gynecologists, reproductive endocrinologists, and embryologists working in the field of infertility. |
| computer-assisted sperm analysis pdf: Office-Based Infertility Practice David B. Seifer, Robert L. Collins, 2002-01-08 Office-Based Infertility Practice is an invaluable resource to all physicians treating infertile couples. The text emphasizes the practice of infertility in the office setting, reflecting the current trend away from the hospital into the outpatient environment. The most current and advanced therapies available are discussed by recognized experts in the field. The first half of the book is devoted to the evaluation and work-up of the infertile couple, including evaluation of the male, female, age-related infertility factors, and the roles of ultrasound, endometrium saline sonography, falloposcopy and diagnostic laparoscopy and hysteroscopy. The second half of the book presents the treatment and operative procedures for the infertile couple, including ovulation induction, IUI, tubal cannulation, treatment of cervical stenosis, the use of office laparoscopy and therapeutic hysteroscopy, male treatment as support for IVF, vas reversals, and testicular biopsy, as well as routine IVF, intratubal gamete transfer, and micromanipulation. With over 60 illustrations, this book is a must for all infertility specialists, obstetrician-gynecologists, fellows, and residents. Its practical, comprehensive approach will be of daily use to the office practitioner treating women of reproductive age. |
| computer-assisted sperm analysis pdf: Germline Development in the Zebrafish Roland Dosch, 2021 This volume details a wide range of methods, ranging from beginner through advanced, used to further study zebrafish and fish germline. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field. |
| computer-assisted sperm analysis pdf: Clinical In Vitro Fertilization C. Wood, A. Trounson, 2012-12-06 Man is entering a new era as a result of advances in human reproduction. Techniques have been developed to assist in the creation of man-artificial insemination and, now, in vitro fertilization (IVF). Soon, other new methods, based upon current advances of the IVF procedure, will develop to improve the quality of human reproduction. The book describes the conceptual framework and details of technique concerned with in vitro fertilization and embryo transfer (ET). Edwards and Steptoe first described the technique of IVF and ET and the subsequent births of two normal babies. Since then, the success rate of the system has been improved by the use of fertility drugs to provide more oocytes and preincubation to mature the oocyte before fertilization. As a result of the continued research from Melbourne and Cambridge, more than 100 babies have been born. A free interchange of information between the Cambridge and Melbourne groups has led to a predictable success rate of 15%-20% per laparoscopy, and infertility centres all over the world are now copying the techniques. It is an appropriate time to inform doctors and scientists to help them understand the various procedures involved in IVF and ET. While many advances will occur in the future, the establishment of high success rates in several of the critical steps in the procedure-oocyte pick-up rate (90%), fertilization (>90%) and early embryo development (70%-90% )-signifies that some of the new techniques are stabilized sufficiently to warrant transmission of information by text, rather than scientific journal. |
| computer-assisted sperm analysis pdf: Clinical Management of Male Infertility Giorgio Cavallini, Giovanni Beretta, 2014-10-20 This book provides andrologists and other practitioners with reliable, up-to-date information on all aspects of male infertility and is designed to assist in the clinical management of patients. Clear guidance is offered on classification of infertility, sperm analysis interpretation and diagnosis. The full range of types and causes of male infertility are then discussed in depth. Particular attention is devoted to poorly understood conditions such as unexplained couple infertility and idiopathic male infertility, but the roles of diverse disorders, health and lifestyle factors and environmental pollution are also fully explored. Research considered stimulating for the reader is highlighted, reflecting the fascinating and controversial nature of the field. International treatment guidelines are presented and the role of diet and dietary supplements is discussed in view of their increasing importance. Clinicians will find that the book’s straightforward approach ensures that it can be easily and rapidly consulted. |
| computer-assisted sperm analysis pdf: Toxicologic Assessment of Jet-Propulsion Fuel 8 National Research Council, Division on Earth and Life Studies, Board on Environmental Studies and Toxicology, Committee on Toxicology, Subcommittee on Jet-Propulsion Fuel 8, 2003-02-14 This report provides a critical review of toxicologic, epidemiologic, and other relevant data on jet-propulsion fuel 8, a type of fuel in wide use by the U.S. Department of Defense (DOD), and an evaluation of the scientific basis of DOD's interim permissible exposure level of 350 mg/m3 |
| computer-assisted sperm analysis pdf: Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms , 2002 |
| computer-assisted sperm analysis pdf: Spermatogenesis Lori Barnard, Kenneth I. Aston, 2012-09-20 Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike. |
| computer-assisted sperm analysis pdf: Cytoskeleton Jose C. Jimenez-Lopez, 2017-05-17 The cytoskeleton is a highly dynamic intracellular platform constituted by a three-dimensional network of proteins responsible for key cellular roles as structure and shape, cell growth and development, and offering to the cell with motility that being the ability of the entire cell to move and for material to be moved within the cell in a regulated fashion (vesicle trafficking). The present edition of Cytoskeleton provides new insights into the structure-functional features, dynamics, and cytoskeleton's relationship to diseases. The authors' contribution in this book will be of substantial importance to a wide audience such as clinicians, researches, educators, and students interested in getting updated knowledge about molecular basis of cytoskeleton, such as regulation of cell vital processes by actin-binding proteins as cell morphogenesis, motility, their implications in cell signaling, as well as strategies for clinical trial and alternative therapies based in multitargeting molecules to tackle diseases, that is, cancer. |
| computer-assisted sperm analysis pdf: Atlas of Comparative Diagnostic and Experimental Hematology Clifford Smith, Alfred Jarecki, 2011-08-15 A vital resource on blood and bone marrow cell morphology in laboratory animal medicine. This fully revised new edition is an essential reference for clinical pathologists in diagnostic laboratories, and medical or veterinary research. The atlas contains over 400 color images of cells from the peripheral blood and bone marrow from a variety of animals encountered in laboratory animal medicine, in health and disease. Key features: New chapter on flow cytometry and its application in terms of routine analyses as a means of identifying abnormalities in cell marker expression, which is of particular relevance for pre-clinical safety assessment Covers the most recent developments in laboratory animal hematology, including parameters measured by the latest generation of analyzers Coverage of a wide range of laboratory animal species, as well as those used in clinical veterinary trials Photomicrographs present normal and abnormal blood cells from a variety of hematological conditions along with descriptive text |
| computer-assisted sperm analysis pdf: Sperm Biology Scott S. Pitnick, Dave J. Hosken, Tim R. Birkhead, 2008-11-21 Sperm Biology represents the first analysis of the evolutionary significance of sperm phenotypes and derived sperm traits and the possible selection pressures responsible for sperm-egg coevolution. An understanding of sperm evolution is fast developing and promises to shed light on many topics from basic reproductive biology to the evolutionary process itself as well as the sperm proteome, the sperm genome and the quantitative genetics of sperm. The Editors have identified 15 topics of current interest and biological significance to cover all aspects of this bizarre, fascinating and important subject. It comprises the most comprehensive and up-to-date review of the evolution of sperm and pointers for future research, written by experts in both sperm biology and evolutionary biology. The combination of evolution and sperm is a potent mix, and this is the definitive account. - The first review survey of this emerging field - Written by experts from a broad array of disciplines from the physiological and biomedical to the ecological and evolutionary - Sheds light on the intricacies of reproduction and the coevolution of sperm, egg and reproductive behavior |
| computer-assisted sperm analysis pdf: WHO Manual for the Standardized Investigation and Diagnosis of the Infertile Male Patrick J. Rowe, Frank H. Comhaire, Timothy B. Hargreave, 2000-03-28 A structured approach to the most-effective clinical management of all forms of male infertility. |
| computer-assisted sperm analysis pdf: Mathematical Biofluiddynamics James Lighthill, 1975-01-01 Addresses external biofluiddynamics concerning animal locomotion and internal biofluiddynamics concerning heat and mass transport. |
| computer-assisted sperm analysis pdf: Artificial Insemination in Poultry P. E. Lake, J. M. Stewart, 1978 |
| computer-assisted sperm analysis pdf: Artificial Insemination in Farm Animals Milad Manafi, 2011 Artificial insemination is used instead of natural mating for reproduction purposes and its chief priority is that the desirable characteristics of a bull or other male livestock animal can be passed on more quickly and to more progeny than if that animal is mated with females in a natural fashion. This book contains under one cover 16 chapters of concise, up-to-date information on artificial insemination in buffalos, ewes, pigs, swine, sheep, goats, pigs and dogs. Cryopreservation effect on sperm quality and fertility, new method and diagnostic test in semen analysis, management factors affecting fertility after cervical insemination, factors of non-infectious nature affecting the fertility, fatty acids effects on reproductive performance of ruminants, particularities of bovine artificial insemination, sperm preparation techniques and reproductive endocrinology diseases are described. This book will explain the advantages and disadvantages of using AI, the various methodologies used in different species, and how AI can be used to improve reproductive efficiency in farm animals. |
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