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| capillary electrophoresis protein analysis: Capillary Electrophoresis of Proteins Tim Wehr, Roberto Rodriguez-Diaz, Mingde Zhu, 1998-10-20 Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions. |
| capillary electrophoresis protein analysis: Capillary Gel Electrophoresis Andras Guttman, László Hajba, 2021-12-04 Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. - Covers all theoretical and practical aspects of capillary gel electrophoresis - Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods - Teaches readers how to use the technology and select methods that are ideal for fundamental problems - Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses |
| capillary electrophoresis protein analysis: Protein Electrophoresis in Clinical Diagnosis David Keren, 2003-09-26 Since the publication of High-Resolution Electrophorsesis and Immunofixation 2e, there have been ever-increasing advances in the analyses of proteins, by electrophoresis in particular. Protein Electrophoresis in Clinical Diagnosis shows the changes in both techniques and interpretation, presenting a comprehensive review of serum protein techniques, |
| capillary electrophoresis protein analysis: Handbook of Capillary Electrophoresis Applications H. Shintani, 2012-12-06 Over the last decade, high performance Capillary electrophoresis (HPCE) has emerged as a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation of both charged and neutral species. The high speed and high separation efficiency which can be attained using any of the various modes of HPCE has resulted in the increased use of the technique in a range of analytical environments. The procedures are, however, still in the early stages of development and several barriers remain to their adoption as the technique of choice for a range of analytical problems. One such barrier is the selection and optimization of the conditions required to achieve reproducible separations of analytes and it is in this area that this new book seeks to give assistance. The book is written by an international team of authors, drawn from both academic and industrial users, and the manufacturers of instruments. At its heart are a number of tables, divided into specific application areas. These give details of published separations of a wide range of archetypal analytes, the successful separation conditions and the matrix in which they were presented. These tables are based on separations reported since 1992 and are fully referenced to the original literature. The tables are supported by discussions of the problems that a particular area presents and the strategies and solutions adopted to overcome them. The general areas covered are biochemistry, pharmaceutical science, bioscience, ion analysis, food analysis and environmental science. |
| capillary electrophoresis protein analysis: Clinical Applications of Capillary Electrophoresis Stephen M. Palfrey, 2008-02-01 In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including urine protein analysis, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, HbA1c, and cathepsin. The protocols offered for DNA studies include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microsatellite instability in cancers. Many of the methods can be automated to replace the more costly and labor-intensive tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. It offers to beginning clinical investigators, as well as established laboratories new to the technique, a representative range of highly practical CE methods-assays that are not only certain to become ever more productive, but are already eminently useful today. |
| capillary electrophoresis protein analysis: Capillary Electrophoresis of Proteins and Peptides Mark A. Strege, Avinash L. Lagu, 2008-02-04 Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5–7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins. |
| capillary electrophoresis protein analysis: CE in Biotechnology: Practical Applications for Protein and Peptide Analyses Anthony B. Chen, Wassim Nashabeh, Timothy Wehr, 2013-04-17 The goal of this book is to show recent developments in the CE analysis of protein pharmaceuticals. It is devoted completely to practical concerns to strengthen the use of CE within the biotechnology industry, highlighting the uses of CE in various areas of product development including formulation studies, process development, product characterization and validated lot release and stability testing. |
| capillary electrophoresis protein analysis: Amino Acid Analysis Michail A. Alterman, Peter Hunziker, 2011-12-02 Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs. In this volume, a reader can find chapters describing general as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry as well as in food analysis, microbiology, marine biology, drug metabolism, even archeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Amino Acid Analysis: Methods and Protocols provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences. |
| capillary electrophoresis protein analysis: Separation Techniques Applied to Omics Sciences Ana Valéria Colnaghi Simionato, 2021-10-09 This book covers liquid chromatography, gas chromatography and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics and foodomics. The fundamentals of each technique are not covered herein. Instead, the recent advances in such techniques are presented focusing on the application to omics analyses and unique aspects in each case. This volume intends to offer wide ranging options available to researchers on omics sciences, and how to integrate them in order to achieve the comprehension of a biological system as a whole. Omic sciences have been of ultimate importance to comprehend the complex biochemical reactions and related events that occurs upon a biological system. The classical central dogma of molecular biology, which states that genetic information flows unidirectionally from DNA to RNA and then to proteins, has been gradually replaced by the systems biology approach. This book presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment, showing that each level of the biological information flux may influence the previous or the subsequent one. |
| capillary electrophoresis protein analysis: Clinical and Forensic Applications of Capillary Electrophoresis John R. Petersen, Amin A. Mohammad, 2001-04-20 John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and non-aqueous electrophoresis. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology. |
| capillary electrophoresis protein analysis: Proteomics in Functional Genomics P. Jolles, Hans Jörnvall, 2000-03-01 A wealth of information has accumulated over the last few years on the human genome. The new insights have completely changed the focus of protein analysis. It is no longer time-consuming analysis of unknown products, but rather selective identifications of individual forms, modifications and processings, and overall analysis of global protein outputs from cells and tissues in health and disease. This book gears to the rising need of sensitive, accurate, and fast separation and identification techniques in proteomics. It discusses current methodologies of modern protein analysis, from isolation and sample preparation, over analysis and identification, to final characterization. Several evaluations concentrate on the now productive approaches of two-dimensional gel electrophoresis and mass spectrometry, but alternative methods and further perspectives are also outlined. The book includes an overlook over current databases to connect protein analysis data with all available information,... |
| capillary electrophoresis protein analysis: Capillary Electrophoresis-Mass Spectrometry James Q. Xia, Lichao Zhang, 2016-11-08 This book covers the latest developments in capillary electrophoresis-mass spectrometry for the analysis of therapeutic proteins. The application of capillary electrophoresis-mass spectrometry (CE-MS) coupling technology in the analysis of recombinant therapeutic proteins is detailed thoroughly. Specific topics include recent developments in coupling capillary electrophoresis with mass spectrometry for the quality control of monoclonal antibody therapeutics, top-down analysis of monoclonal antibody using the CE-MS platform, and detection of host cell protein impurities. Comprehensive characterization of antibody-drug conjugates (ADCs) by coupling capillary electrophoresis with mass spectrometry is also covered. This is an ideal book for scientists in the life science and biopharmaceutical industry who are working on characterizing the PTMs of monoclonal antibodies, as well as graduate students and researchers in the separation science and biological mass spectrometry fields. |
| capillary electrophoresis protein analysis: Handbook of Capillary Electrophoresis, Second Edition James P. Landers, 1996-12-23 Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science. |
| capillary electrophoresis protein analysis: Capillary Electrophoresis - Mass Spectrometry (CE-MS) Gerhardus de Jong, 2016-09-13 Diese Monographie bietet einen vollständigen Überblick über die Prinzipien und Anwendungen der Kapillarelektrophorese (CE) und der Massenspektrometrie (MS) und legt den Nachdruck insbesondere auf Kopplungsschnittstellen. Ausführlich erläutert werden auch alle relevanten Substanzklassen. Ein einzigartiges Wissenskompendium für alle, die sich CE-MS beschäftigen! |
| capillary electrophoresis protein analysis: Capillary Electrophoresis Paul D. Grossman, Joel C. Colburn, 2012-12-02 This book is designed to be a practical guide, used by wide audience, including those new to CE, those more experienced, routine users, those interested in technology development, and those involved with applications research. References have been emphasized to allow the reader to explore the detailed specifics and theoretical foundations.This book draws together the rapidly evolving, diverse, and multidisciplinary subject of capillary electrophoresis (CE). It is designed as a practical guide to be used by a wide audience, including those new to CE as well as more experienced users. This volume presents the capabilities, limitations, potentials, and future challenges facing each area of CE. Key aspects of this technique, such as high resolution capability, full automation, high speed separations, quantification of nanoliter sample volumes, and simultaneous multiple detection capabilities are presented in a concise and logical fashion. This book is designed to help you make the most of your CE separations, and includes comprehensive information on:Electroosmosis, separation efficiency, and Joule heatingDetection methodsIn-depth discussion of the separation principles and capabilities of the major modes of CESieving gel electrophoresisIsoelectric focusingFree solution CEMicellar electrokinetic capillary chromatographyEntangled polymer matrix-based separationDetailed treatment of the application of CE to a wide range of molecules, supplemented with extensive hands-on illustrations |
| capillary electrophoresis protein analysis: Capillary Zone Electrophoresis František Foret, Ludmila Křivánková, Petr Boček, 1993 |
| capillary electrophoresis protein analysis: Capillary Electrophoresis and Microchip Capillary Electrophoresis Carlos D. García, Karin Y. Chumbimuni-Torres, Emanuel Carrilho, 2013-02-01 Explores the benefits and limitations of the latest capillary electrophoresis techniques Capillary electrophoresis and microchip capillary electrophoresis are powerful analytical tools that are particularly suited for separating and analyzing biomolecules. In comparison with traditional analytical techniques, capillary electrophoresis and microchip capillary electrophoresis offer the benefits of speed, small sample and solvent consumption, low cost, and the possibility of miniaturization. With contributions from a team of leading analytical scientists, Capillary Electrophoresis and Microchip Capillary Electrophoresis explains how researchers can take full advantage of all the latest techniques, emphasizing applications in which capillary electrophoresis has proven superiority over other analytical approaches. The authors not only explore the benefits of each technique, but also the limitations, enabling readers to choose the most appropriate technique to analyze a particular sample. The book's twenty-one chapters explore fundamental aspects of electrophoretically driven separations, instrumentation, sampling techniques, separation modes, detection systems, optimization strategies for method development, and applications. Specific topics include: Critical evaluation of the use of surfactants in capillary electrophoresis Sampling and quantitative analysis in capillary electrophoresis Capillary electrophoresis with electrochemical detection Overcoming challenges in using microchip electrophoresis for extended monitoring applications Capillary electrophoresis of intact unfractionated heparin and related impurities Microchip capillary electrophoresis for in situ planetary exploration Each chapter begins with an introduction and ends with conclusions as well as references to the primary literature. Novices to the field will find this book an easy-to-follow introduction to core capillary electrophoresis techniques and methods. More experienced investigators can turn to the book for troubleshooting tips and expert advice to guide them through the most advanced applications. |
| capillary electrophoresis protein analysis: Paper Electrophoresis G. E. W. Wolstenholme, Elaine C. P. Millar, 2009-09-14 The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world. |
| capillary electrophoresis protein analysis: Principles and Reactions of Protein Extraction, Purification, and Characterization Hafiz Ahmed, Hafiz Ahmed PhD, 2017-07-27 Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different |
| capillary electrophoresis protein analysis: Proteomic and Metabolomic Approaches to Biomarker Discovery Haleem J. Issaq, 2013-05-20 Proteomic and Metabolomic Approaches to Biomarker Discovery demonstrates how to leverage biomarkers to improve accuracy and reduce errors in research. Disease biomarker discovery is one of the most vibrant and important areas of research today, as the identification of reliable biomarkers has an enormous impact on disease diagnosis, selection of treatment regimens, and therapeutic monitoring. Various techniques are used in the biomarker discovery process, including techniques used in proteomics, the study of the proteins that make up an organism, and metabolomics, the study of chemical fingerprints created from cellular processes. Proteomic and Metabolomic Approaches to Biomarker Discovery is the only publication that covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution. The book describes methods, and presents a standard operating procedure for sample selection, preparation, and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process. A vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals, and graduate students, Proteomic and Metabolomic Approaches to Biomarker Discovery provides the information needed to reduce clinical error in the execution of research. - Describes the use of biomarkers to reduce clinical errors in research - Includes techniques from a range of biomarker discoveries - Covers all steps involved in biomarker discovery, from study design to study execution |
| capillary electrophoresis protein analysis: Capillary Electrophoresis Philippe Schmitt-Kopplin, 2008-02-25 This book presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. It includes practical details which are hard to find elsewhere. The volume is intended for beginners in the field and provides an overview of the technique and a starting point for the exploration of the defined literature on different application topics. |
| capillary electrophoresis protein analysis: Antibody Glycosylation Marija Pezer, 2021-10-22 This book summarizes recent advances in antibody glycosylation research. Covering major topics relevant for immunoglobulin glycosylation - analytical methods, biosynthesis and regulation, modulation of effector functions - it provides new perspectives for research and development in the field of therapeutic antibodies, biomarkers, vaccinations, and immunotherapy. Glycans attached to both variable and constant regions of antibodies are known to affect the antibody conformation, stability, and effector functions. Although it focuses on immunoglobulin G (IgG), the most explored antibody in this context, and unravels the natural phenomena resulting from the mixture of IgG glycovariants present in the human body, the book also discusses other classes of human immunoglobulins, as well as immunoglobulins produced in other species and production systems. Further, it reviews the glycoanalytical methods applied to antibodies and addresses a range of less commonly explored topics, such as automatization and bioinformatics aspects of high-throughput antibody glycosylation analysis. Lastly, the book highlights application areas ranging from the ones already benefitting from antibody glycoengineering (such as monoclonal antibody production), to those still in the research stages (such as exploration of antibody glycosylation as a clinical or biological age biomarker), and the potential use of antibody glycosylation in the optimization of vaccine production and immunization protocols. Summarizing the current knowledge on the broad topic of antibody glycosylation and its therapeutic and biomarker potential, this book will appeal to a wide biomedical readership in academia and industry alike. Chapter 4 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. |
| capillary electrophoresis protein analysis: Capillary Electrophoresis of Proteins and Peptides Nguyet Thuy Tran, Myriam Taverna, 2016-07-30 This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study. |
| capillary electrophoresis protein analysis: Peptide and Protein Drug Analysis Ronald Reid, 1999-11-12 Furthering efforts to simulate the potency and specificity exhibited by peptides and proteins in healthy cells, this remarkable reference supplies pharmaceutical scientists with a wealth of techniques for tapping the enormous therapeutic potential of these molecules-providing a solid basis of knowledge for new drug design. Provides a broad, comprehensive overview of peptides and proteins as mediators of cell movement, proliferation, differentiation, and communication. Written by more than 50 leading international authorities, Peptides and Protein Drug Analysis discusses strategies for dealing with the complexity of peptides and proteins in conformational flexibility and amino acid sequence variability analyzes drug formulations facilitated by solid-phase peptide synthesis and recombinant DNA technology examines chemical purity analysis by high-pressure chromatographic, capillary electrophoretic, gel electrophoretic, and isoelectric focusing methods highlights drug design elements derived from protein folding, bioinformatics, and computational chemistry demonstrates uses of unnatural mutagenesis and combinatorial chemistry explores mass spectrometry, protein sequence, and carbohydrate analysis illustrates bioassays and other new functional analysis methods surveys spectroscopic techniques such as ultraviolet, fluorescence, Fourier transform infrared, and nuclear magnetic resonance (NMR) addresses ways of distinguishing between levels of therapeutic and endogenous agents in cells reviews structural analysis tools such as ultracentrifugation and light, X-ray, and neutron scattering and more! Featuring over 3400 bibliographic citations and more than 500 tables, equations, and illustrations, Peptide and Protein Drug Analysis is a must-read resource for pharmacists; pharmacologists; analytical, organic, and pharmaceutical chemists; cell and molecular biologists; biochemists; and upper-level undergraduate and graduate students in these disciplines. |
| capillary electrophoresis protein analysis: Capillary Electrophoresis Heinz Engelhardt, Wolfgang Beck, Thomas Schmitt, 2012-06-12 Capillary electrophoresis (CE), also designated by the acronym HPCE for High Per formance Capillary Electrophoresis is a rapidly growing analytical separation method. It unites the separation technique of classical electrophoresis on plates with the instrumental methods of chromatography with respect to direct detection of the solutes separated in the capillary and their ready identification and quantification. The initial problems of inadequate reproducibility in quantitative analysis, due to the necessity of handling extremely small volumes, have largely been solved in the sec ond generation commercial instruments. Hence, a rapid and reliable separation sys tem is available for ionic compounds from the smallest cation (the lithium ion) up to poly anions with molecular weights ranging in the millions (such as DNA molecules). The methods of gel electrophoresis and isoelectric focusing can be readily extended to separation techniques carried out in a capillary. For nonionic compounds an addi tional separation method is available in the form of micellar electrokinetic chroma tography (MEKC). This involves a true chromatographic separation process because the distribution of the analytes between the buffer and the micelles is superimposed on the electrophoretic migration, which contributes substantially to the selectivity. |
| capillary electrophoresis protein analysis: Protein Purification Jan-Christer Janson, Lars Rydén, 1998 This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis. |
| capillary electrophoresis protein analysis: Methods for Structural Analysis of Protein Pharmaceuticals Wim Jiskoot, Daan Crommelin, 2005-12-05 Protein pharmaceuticals form a fast-growing category in the arsenal of drugs. This book explores the nature of different analytical techniques and the way in which they are related to pharmaceutical proteins. In addition to serving the analytical chemist, this book is needed by the formulation scientist who is responsible for design and formulation of a pharmaceutical protein that can be monitored during production and over time. |
| capillary electrophoresis protein analysis: Capillary Electrophoresis Technology Norberto A. Guzman, 1993-08-10 Tracing the sequence of observations that has led to current understanding in the field, this reference presents the basic concepts, instrumentation and applications of capillary electrophoresis, examining its many features such as high-power resolution, high-mass sensitivity, overall sensitivity and low-sample volume requirements. This work highlights the use of capillary elctrophoresis for the identification, separation, detection and characterization of substances on the molecular counting level. Illustrating the major technical maneuvers for common operations and applications, Capillary Electrophoresis Technology outlines the theoretical concepts and mathematical expressions of capillary electrophoresis; describes advances in instrumentation hardware and detection systems. It explains the advantages and limitations of the different variants of capillary electrophoresis; and provides extra coverage of areas in which capillary electrophoresis has grown increasingly popular, including the identification and characterization of small molecules and macromolecules. Written by experts in the field, this book is aimed at analytical and clinical chemists and biochemists, chemical engineers, biologists, pharmacists, biotechnologists and students in these disciplines. |
| capillary electrophoresis protein analysis: Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques James P. Landers, 2007-12-18 Now in its third edition, this bestselling work continues to offer state-of-the-art information on the development and employment of capillary electrophoresis. With special emphasis on microseparations and microfluidics, it features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Enhanced by contributions from leading international experts, the Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition remains a seminal reference for the chemistry, biology, and engineering fields. |
| capillary electrophoresis protein analysis: Capillary Electromigration Separation Methods Colin F. Poole, 2018-04-13 Capillary Electromigration Separation Methods is a thorough, encompassing reference that not only defines the concept of contemporary practice, but also demonstrates its implementation in laboratory science. Chapters are authored by recognized experts in the field, ensuring that the content reflects the latest developments in research. Thorough, comprehensive coverage makes this the ideal reference for project planning, and extensive selected referencing facilitates identification of key information. The book defines the concept of contemporary practice in capillary electromigration separation methods, also discussing its applications in small mass ions, stereoisomers, and proteins. - Edited and authored by world-leading capillary electrophoresis experts - Presents comprehensive coverage on the subject - Includes extensive referencing that facilitates the identification of key research developments - Provides more than 50 figures and tables that aid in the retention of key concepts |
| capillary electrophoresis protein analysis: Food Toxicants Analysis Yolanda Picó, 2007-02-07 Food Toxicants Analysis covers different aspects from the field of analytical food toxicology including emerging analytical techniques and applications to detect food allergens, genetically modified organisms, and novel ingredients (including those of functional foods). Focus will be on natural toxins in food plants and animals, cancer modulating substances, microbial toxins in foods (algal, fungal, and bacterial) and all groups of contaminants (i.e., pesticides), persistent organic pollutants, metals, packaging materials, hormones and animal drug residues. The first section describes the current status of the regulatory framework, including the key principles of the EU food law, food safety, and the main mechanisms of enforcement. The second section addresses validation and quality assurance in food toxicants analysis and comprises a general discussion on the use of risk analysis in establishing priorities, the selection and quality control of available analytical techniques. The third section addresses new issues in food toxicant analysis including food allergens and genetically modified organisms (GMOs). The fourth section covers the analysis of organic food toxicants.* step-by-step guide to the use of food analysis techniques* eighteen chapters covering emerging fields in food toxicants analysis* assesses the latest techniques in the field of inorganic analysis |
| capillary electrophoresis protein analysis: Unified Separation Science J. Calvin Giddings, 1991-01-16 Unifies the complex welter of techniques used for chemical separations by clearly formulating the concepts that are common to them. The mass transport phenomena underlying all separation processes are developed in a simple physical-mathematical form. The limitations and optimum performance of alternative separation techniques and the factors enhancing and limiting separation power can thus be described and explored. Generously illustrated and contains numerous exercises. Long awaited in the scientific community, it breaks new ground in understanding separation processes. |
| capillary electrophoresis protein analysis: Electrophoresis in Practice Reiner Westermeier, 2016-05-16 Electrophoresis in Practice ist seit mehr als zwei Jahrzehnten das Standardwerk in der Elektrophorese. Die 5. Auflage wurde sorgfältig überarbeitet und beinhaltet nun ein erweitertes Kapitel zu Mikromethoden und der chipgebundenen Elektrophorese. |
| capillary electrophoresis protein analysis: Neuroproteomics Oscar Alzate, 2009-10-26 In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg |
| capillary electrophoresis protein analysis: Chemical Cytometry Chang Lu, 2010-03-08 A comprehensive introduction to the novel approach for biochemical analysis at the single cell level that covers recent methods for fractionation, detection and analysis of molecules from individual cells. |
| capillary electrophoresis protein analysis: Advanced Topics in Forensic DNA Typing: Interpretation John M. Butler, 2014-07-28 Advanced Topics in Forensic DNA Typing: Interpretation builds upon the previous two editions of John Butler's internationally acclaimed Forensic DNA Typing textbook with forensic DNA analysts as its primary audience. Intended as a third-edition companion to the Fundamentals of Forensic DNA Typing volume published in 2010 and Advanced Topics in Forensic DNA Typing: Methodology published in 2012, this book contains 16 chapters with 4 appendices providing up-to-date coverage of essential topics in this important field. Over 80 % of the content of this book is new compared to previous editions. - Provides forensic DNA analysts coverage of the crucial topic of DNA mixture interpretation and statistical analysis of DNA evidence - Worked mixture examples illustrate the impact of different statistical approaches for reporting results - Includes allele frequencies for 24 commonly used autosomal STR loci, the revised Quality Assurance Standards which went into effect September 2011 |
| capillary electrophoresis protein analysis: Analytical Determination of Nicotine and Related Compounds and their Metabolites J.W. Gorrod, P. Jacob III, 1999-11-02 This book provides for the first time a single comprehensive source of information on the analytical chemistry of nicotine and related alkaloids. The editors have brought together scientists from academia and the tobacco industry to describe the state-of-the-art of the chemistry and analytical methods for measurement of nicotine. Both the scope and detail of the book are impressive. Chapters describe the history, pharmacology and toxicology of nicotine, the biosynthesis of nicotine and other alkaloids in the tobacco plant, the general chemistry of nicotine and the analytical methodologies that have been used to measure nicotine and related alkaloids in biological specimens, in tobacco and pharmaceutical products and in tobacco smoke. There is also a comprehensive review of the chemistry and toxicology of nicotine-derived nitrosamines, an important class of tobacco carcinogens. |
| capillary electrophoresis protein analysis: Essentials of Glycobiology Ajit Varki, Maarten J. Chrispeels, 1999 Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. Essentials of Glycobiology describes their biogenesis and function and offers a useful gateway to the understanding of glycans. |
| capillary electrophoresis protein analysis: Haemoglobinopathy Diagnosis Barbara J. Bain, 2020-02-28 An updated, essential guide for the laboratory diagnosis of haemoglobin disorders This revised and updated third edition of Haemoglobinopathy Diagnosis offers a comprehensive review of the practical information needed for an understanding of the laboratory diagnosis of haemoglobin disorders. Written in a concise and approachable format, the book includes an overview of clinical and laboratory features of these disorders. The author focuses on the selection, performance, and interpretation of the tests that are offered by the majority of diagnostic laboratories. The book also explains when more specialist tests are required and explores what specialist referral centres will accomplish. The information on diagnosis is set in a clinical context. The third edition is written by a leading haematologist with a reputation for educational excellence. Designed as a practical resource, the book is filled with illustrative examples and helpful questions that can aide in the retention of the material presented. Additionally, the author includes information on the most recent advances in the field. This important text: • Contains a practical, highly illustrated, approach to the laboratory diagnosis of haemoglobin disorders • Includes “test-yourself” questions and provides an indispensable tool for learning and teaching • Presents new material on antenatal screening/prenatal diagnostic services • Offers myriad self-assessment case studies that are ideal for the trainee Written for trainees and residents in haematology, practicing haematologists, and laboratory scientists, Haemoglobinopathy Diagnosis is an essential reference and learning tool that provides a clear basis for understanding the diagnosis of haemoglobin disorders. |
| capillary electrophoresis protein analysis: Capillary Electrophoresis: Principles and Practice Reinhard Kuhn, Sabrina Hoffstetter-Kuhn, 2013-03-07 Capillary electrophoresis (CE) is a brand-new analytical me- thod with the capability of solving many analytical separa- tion problems very fast and economically. This method gives new information about the investigated substances which can- not easily be obtained by other means. CE has become an established method only recently, but will be implemented in almost every analytical laboratory in industry, service units and academia in the near future. The most important fields of CE application are pharmaceutical and biochemical research and quality control. The authors have exhaustive practical experience in the application of CE methods in the pharmaceutical industry and provide the reader with a comprehensive treatment of this method. The main focus is on how to solve problems when applying CE in the laboratory. Physico-chemical theory is only dealt with in depth when necessary to understand the underlying separation mechanisms in order to solve your problems at the analytical bench. An addendum includes tables on the preparation of buffers and recommended further reading. |
1053 CAPILLARY ELECTROPHORESIS - US Pharmacopeia (USP)
This chapter provides guidance and procedures used for characterization of biotechnology-derived articles by capillary electrophoresis. This chapter is harmonized with the …
Analysis of Protein Therapeutics by Capillary Electrophoresis
This chapter is divided into four main sections based on the distinct modes of ca pillary electrophoresis discussed: (i) capil lary electrophoresis-sodium dodecylsul fate, (ii) capillary …
Capillary Electrophoresis - Bio-Rad
Capillary electrophoresis allows you to automate the analysis of a wide range of compounds, including peptides, proteins, nucleic acids, oligonucleotides, and pharmaceuticals.
Challenging applications of capillary electrophoresis in ...
After decades of development and refinement, the separation of proteins by capillary electrophoresis (CE) has moved beyond the R&D laboratory into the manufacturing quality …
Native Capillary Electrophoresis-Mass Spectrometry for …
In this review, we present an overview of current native CE-MS technology.
Christian W. Huck and Günther K. Bonn - Springer
This chapter describes the basic principles of protein analysis by capillary electrophoresis, and provides an overview of the literature and thus a comprehensive summary of special topics in …
360643: Introduction to Capillary Electrophoresis - SCIEX
This handbook, the first of a series on modern high performance capillary electrophoresis (CE), is intended for scientists who are contemplating use of or have recently started using this rapidly …
Capillary Electrophoresis in Quality Control PART II: CE-SDS
protein variants by a sieving matrix (i.e. polymer) in a constant electric field, with the following critical method parameters: SDS labeling technique, sieving matrix, and electrophoretic …
CAPILLARY ELECTROPHORESIS - kuk.ac.in
Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility with the use of an applied voltage. CE is performed in a small glass …
Fully Automated Two-dimensional Capillary Electrophoresis …
We report a system for automated protein analysis. In the system, proteins are labeled with the fluorogenic reagent 3-(2-furoyl)quinoline-2-carboxaldehyde, which reacts with lysine residues …
14 Protein analysis by capillary electrophoresis - Springer
Zone electrophoresis performed on supporting media such as agarose and polyacrylamide gels provides a powerful and indispensable tool for the separation and characterization of proteins.
727484: Separation of Proteins and Peptides by Capillary
HPLC and CE (or, in a broader sense, chromatography and electrophoresis) fulfill the above requirements. These techniques provide fully automated, computer-controlled, quantitative …
Determination of protein purity and heterogeneity by capillary …
To determine the purity and heterogeneity of protein samples, two capillary-based methods are proposed – capillary gel electrophoresis (CGE) and capillary isoelectric focusing (cIEF) . The …
Protein Cross-Linking Capillary Electrophoresis for Protein …
Resolution of free proteins from protein complexes. Complex Resolution Hsp70-Bag3 1.0 Hsp90 homodimer 2.0 Lysozyme-anti-lysozyme 1.1
Selection of Buffers in Capillary Zone Electrophoresis
Separating proteins and peptides remains one of the most challenging tasks in analy tical chemistry. In the past, conventional gel electrophoresis and high performance liquid …
High-throughput capillary electrophoresis-sodium dodecyl
CE-SDS purity assays are required at different stages of the antibody development lifecycle, including stages of screening through characterization, production process development, …
Dependable Denaturing Protein Electrophoresis - Agilent
By using discrete capillaries for separations, purified antibodies, secreted proteins, membrane proteins, and crude lysates can be consistently analyzed. A streamlined workflow quickly …
How is Capillary Electrophoresis (CE) Being Used in Gene …
Capillary electrophoresis as a technique is widely used in profiling protein-based products and has a track record of being used in oligonucleotide analysis. In this poster, a few examples are …
Strengths of PA 800 Plus for Multi‐Attribute Protein Analyzer
Capillary Electrophoresis is a well-accepted analytical separation technology in many different environments within the biologics domains.
Capillary Electrophoresis in Quality Control: Part I ... - SCIEX
In the first part of this series of articles, we will illustrate an overview of CE in a modern quality control laboratory.
1053 CAPILLARY ELECTROPHORESIS - US Pharmacopeia …
This chapter provides guidance and procedures used for characterization of biotechnology-derived articles by capillary electrophoresis. This chapter is harmonized with the …
Analysis of Protein Therapeutics by Capillary Electrophoresis …
This chapter is divided into four main sections based on the distinct modes of ca pillary electrophoresis discussed: (i) capil lary electrophoresis-sodium dodecylsul fate, (ii) capillary …
Capillary Electrophoresis - Bio-Rad
Capillary electrophoresis allows you to automate the analysis of a wide range of compounds, including peptides, proteins, nucleic acids, oligonucleotides, and pharmaceuticals.
Challenging applications of capillary electrophoresis in ...
After decades of development and refinement, the separation of proteins by capillary electrophoresis (CE) has moved beyond the R&D laboratory into the manufacturing quality …
Native Capillary Electrophoresis-Mass Spectrometry for …
In this review, we present an overview of current native CE-MS technology.
Christian W. Huck and Günther K. Bonn - Springer
This chapter describes the basic principles of protein analysis by capillary electrophoresis, and provides an overview of the literature and thus a comprehensive summary of special topics in …
360643: Introduction to Capillary Electrophoresis - SCIEX
This handbook, the first of a series on modern high performance capillary electrophoresis (CE), is intended for scientists who are contemplating use of or have recently started using this rapidly …
Capillary Electrophoresis in Quality Control PART II: CE-SDS …
protein variants by a sieving matrix (i.e. polymer) in a constant electric field, with the following critical method parameters: SDS labeling technique, sieving matrix, and electrophoretic …
CAPILLARY ELECTROPHORESIS - kuk.ac.in
Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility with the use of an applied voltage. CE is performed in a small glass …
Fully Automated Two-dimensional Capillary Electrophoresis …
We report a system for automated protein analysis. In the system, proteins are labeled with the fluorogenic reagent 3-(2-furoyl)quinoline-2-carboxaldehyde, which reacts with lysine residues …
14 Protein analysis by capillary electrophoresis - Springer
Zone electrophoresis performed on supporting media such as agarose and polyacrylamide gels provides a powerful and indispensable tool for the separation and characterization of proteins.
727484: Separation of Proteins and Peptides by Capillary
HPLC and CE (or, in a broader sense, chromatography and electrophoresis) fulfill the above requirements. These techniques provide fully automated, computer-controlled, quantitative …
Determination of protein purity and heterogeneity by capillary …
To determine the purity and heterogeneity of protein samples, two capillary-based methods are proposed – capillary gel electrophoresis (CGE) and capillary isoelectric focusing (cIEF) . The …
Protein Cross-Linking Capillary Electrophoresis for Protein …
Resolution of free proteins from protein complexes. Complex Resolution Hsp70-Bag3 1.0 Hsp90 homodimer 2.0 Lysozyme-anti-lysozyme 1.1
Selection of Buffers in Capillary Zone Electrophoresis
Separating proteins and peptides remains one of the most challenging tasks in analy tical chemistry. In the past, conventional gel electrophoresis and high performance liquid …
High-throughput capillary electrophoresis-sodium dodecyl
CE-SDS purity assays are required at different stages of the antibody development lifecycle, including stages of screening through characterization, production process development, …
Dependable Denaturing Protein Electrophoresis - Agilent
By using discrete capillaries for separations, purified antibodies, secreted proteins, membrane proteins, and crude lysates can be consistently analyzed. A streamlined workflow quickly …
How is Capillary Electrophoresis (CE) Being Used in Gene …
Capillary electrophoresis as a technique is widely used in profiling protein-based products and has a track record of being used in oligonucleotide analysis. In this poster, a few examples are …
Strengths of PA 800 Plus for Multi‐Attribute Protein Analyzer
Capillary Electrophoresis is a well-accepted analytical separation technology in many different environments within the biologics domains.
