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| coomassie brilliant blue r 250 staining solution: Guide to Protein Purification Murray P. Deutscher, 1990 Guide to Protein Purification , designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features* Detailed procedures newly written for this volume* Extensive practical information* Rationale and strategies for protein and enzyme purification* Personal perspectives on enzyme purification by eminent researchersAmong the Topics Covered* General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure. |
| coomassie brilliant blue r 250 staining solution: Protein Gel Detection and Imaging Biji T. Kurien, R. Hal Scofield, 2019-08-24 This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Protein Gel Detection and Imaging: Methods and Protocols is a valuable resource for expert and novice scientists and researchers who are interested in learning and experimenting with this field. |
| coomassie brilliant blue r 250 staining solution: Western Blotting for the Non-Expert Biji T. Kurien, 2021-07-24 This book fills the need for a simplified text covering western blotting protocols aimed not just at high school and college students, but the researcher with little to no experience in these techniques. It provides the principles, basic methodology, and tips and tricks to avoiding the common pitfalls of western blotting. The book also introduces simple protocols that can transform western blotting into a fun method, such as sending secret messages on membranes or using nitrocellulose membrane as a canvas for art. In addition to the techniques, this book also covers the history of western blotting, which originated from the development of the blotting of DNA. It then delves into the importance of protein blotting, brought to the fore by the fact that the procedure has been evolving constantly since its inception in 1979, and the fact that the scientific community is faced with a multitude of ways and means of transferring proteins to membranes.. |
| coomassie brilliant blue r 250 staining solution: Protein Structure Analysis Roza Maria Kamp, Theodora Choli-Papadopoulou, Brigitte Wittmann-Liebold, 2012-12-06 Protein Structure Analysis - Preparation and Characterization is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on Microsequence Analysis of Proteins held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered. |
| coomassie brilliant blue r 250 staining solution: Techniques of Sample Preraration for Liquid Scintillation Counting+Isoelectric Focusing , 1976-01-01 Techniques of Sample Preraration for Liquid Scintillation Counting+Isoelectric Focusing |
| coomassie brilliant blue r 250 staining solution: Guide to Protein Purification Richard R Burgess, Murray P. Deutscher, 2009-11-03 Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This Guide to gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. - Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology - Assembles chapters on both common and less common relevant techniques - Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process |
| coomassie brilliant blue r 250 staining solution: Electrophoresis Budin Michov, 2020-09-07 Electrophoresis is a straightforward but informative analytical method used in biochemistry, biology and medicine. This book combines a detailed discussion of theory and technical application with an elaborate section on troubleshooting and problem solving in electrophoresis. Therefore the book is an important guide for both students and scientists. |
| coomassie brilliant blue r 250 staining solution: Protein Electrophoresis Biji T. Kurien, R. Hal Scofield, 2012-05-16 Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field. |
| coomassie brilliant blue r 250 staining solution: The Proteome Revisited A. Stoyanov, M. Zhukov, Pier Giorgio Righetti, 2001-10-25 The book deals with the theory and practice of all electrophoretic steps leading to proteome analysis, i.e. isoelectric focusing (including immobilized pH gradients), sodium dodecyl sulphate electrophoresis (SADS-PAGE) and finally two-dimensional maps. It is a reasoned collection of all modern, relevant, up-to-date methodologies leading to successful fractionation, analysis and characterization of every polypeptide spot in 2-D map analysis. It includes chapters on the most sophisticated mass spectrometry developments and it helps the reader in navigating through the most important databases in proteome analysis, including step by step tours in selected sites. Yet, this book's unique strength and feature is the fact that it combines not only practice (in common with any other book on this topic) but also theory, by giving a detailed treatment on the most advanced theoretical treatments of steady-state techniques, such as isoelectric focusing and immobilized pH gradients. A lot of this theory is newly developed and presented to the public for the first time. Thus, this book should satisfy not only the needs of every day practitioners, but also the desires of the most advanced theoreticians in the field, who will surely appreciate the novel theories presented here. Also the methodological section contains several as yet unpublished protocols, correcting some of the existing ones and showing the pitfall and limitations of even well ingrained protocols in proteome analysis, which are here critically re-evaluated for the first time. |
| coomassie brilliant blue r 250 staining solution: Introduction to Biophysical Methods for Protein and Nucleic Acid Research Jay A. Glasel, Murray P. Deutscher, 1995-11-20 The first of its kind, Introduction to Biophysical Methods for Protein and Nucleic Acid Research serves as a text for the experienced researcher and student requiring an introduction to the field. Each chapter presents a description of the physical basis of the method, the type of information that may be obtained with the method, how data should be analyzed and interpreted and, where appropriate, practical tips about procedures and equipment.Key Features* Modern Use of Mass Spectroscopy* NMR Spectroscopy* Molecular Modeling and Graphics* Macintosh and DOS/Windows 3.x disks |
| coomassie brilliant blue r 250 staining solution: Proteomics Today Mahmoud H. Hamdan, Pier G. Righetti, 2005-05-13 The last few years have seen an unprecedented drive toward theapplication of proteomics to resolving challenging biomedical andbiochemical tasks. Separation techniques combined with modern massspectrometry are playing a central role in this drive. This bookdiscusses the increasingly important role of mass spectrometry inproteomic research, and emphasizes recent advances in the existingtechnology and describes the advantages and pitfalls as well. * Provides a scientifically valid method for analyzing theapproximatey 500,000 proteins that are encoded in the humangenome * Explains the hows and whys of using mass spectrometry inproteomic analysis * Brings together the latest approaches combining separationtechniques and mass spectrometry and their application in proteomeanalysis * Comments on future challenges and how they may be addressed * Includes sections on troubleshooting |
| coomassie brilliant blue r 250 staining solution: Electrophoresis Fundamentals Budin Michov, 2022-04-04 Electrophoresis methods are simple and informative analytical methods, which are used in biochemistry and medicine. They are necessary for precise investigation of proteins and nucleic acids (DNA and RNA). Electrophoresis is practiced in chemical, biochemical, and clinical laboratories, as well as in forensic medicine laboratories. This book will discuss the fundamentals of electrophoresis methods at the graduate level. |
| coomassie brilliant blue r 250 staining solution: Gel Electrophoresis and Isoelectric Focusing of Proteins Robert C. Allen, Calvin A. Saravis, H.R. Maurer, 2019-05-20 No detailed description available for Gel Electrophoresis and Isoelectric Focusing of Proteins. |
| coomassie brilliant blue r 250 staining solution: Essentials of Biotechnology Ulhas K. Patil, Kalyani Muskan, 2009 Essentials of Biotechnology is meant for undergraduate biotechnology and life sciences students. The book discusses the basics of interdisciplinary subjects which is required for developing the conceptual understanding in biotechnology and to acquire research attitude. It elaborates fundamental concepts which are absolutely necessary for budding biotechnologists. It is an attempt to cover broad spectrum of biological dimensions with biotechnological exploration. Section-I elaborates theoretical aspects of basic biology, biochemistry, microbiology, molecular biology with correlation to modern applied aspects.Section-II is grounded in the experimental approach. Each experiment is described with sufficient details. The figures and tables provided with experiments will be helpful to the students and the instructor for better understanding of the scientific principles and skillful execution of the experiments. |
| coomassie brilliant blue r 250 staining solution: Chemical Tools in Microbiology 1 , 2022-03-22 Clinical Microbiology, Volume 664 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Synthesis of chemical probes to study bacterial adenylating enzymes, Fluoroquinolone-derived fluorescent probes for studies of bacterial penetration and efflux, Combining informatics with ABPP to identify serine hydrolyses in bacteria, A Ligand Selection Strategy Identifies Chemical Probes Targeting the Proteases of SARS-CoV-2, Activity-based probes for bacterial histidine kinases, Metabolomic approaches to enzyme function and pathway discovery, Identification of bile salt hydrolase activity in gut microbiota, and much more. Other chapters cover Multiplex fluorescence screening and identification using multiplex TMT, Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via SPR, Site-Specific Siderocalin Binding to Ferric and Ferric-Free Enterobactin As Revealed by Mass Spectrometry, Proteomics of short-chain fatty acid probes in Salmonella, Development and application of highly sensitive labeling reagents for amino acids, and a variety of other timely topics. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology serials - Updated release includes the latest information on Chemical Microbiology |
| coomassie brilliant blue r 250 staining solution: Bacterial Pathogenesis, Part C: Identification, Regulation and Function of Virulence Factors , 2002-12-05 The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.Key Features* Presents alternatives to mammalian model systems* Discusses virulence and essential gene identification* Defines global gene expression |
| coomassie brilliant blue r 250 staining solution: Plant Biotechnology and Molecular Biology : A Laboratory Manual M.S. Punia, 2018-01-01 The book, “A Laboratory Manual of Plant Biotechnology and Molecular Biology” comprises of workable laboratory protocols for a large number of techniques related to plant biotechnology, genetic engineering and molecular biology. This includes plant cell and tissue culture, callus and suspension culture, anther culture, ovule culture, embryo culture, Cryopreservation, Isolation of Plant protoplasts, Protoplast culture and regeneration, production of somatic hybrids through protoplast fusion, gene transformation using Agrobacterium as vector, direct gene transfer using biolistic gun, Isolation of plant and organells DNA, construction and screening of genomic DNA libraries, Molecular markers like RFLP, RAPD, SCARS and CAPS, DNA sequencing, RNA isolation and northern blotting, Isolation of proteins and western blotting etc. The manual is prepared with the objective to cater the needs of post- graduate students as well as for scientists working in the disciplines of Plant Breeding, Genetics, Botany, Plant physiology, Biochemistry, Plant Biotechnology, Molecular Biology etc. It gives an update on some well established methods and presents reliable protocols. |
| coomassie brilliant blue r 250 staining solution: Gel Electrophoresis Sameh Magdeldin, 2012-04-04 Most will agree that gel electrophoresis is one of the basic pillars of molecular biology. This coined terminology covers a myriad of gel-based separation approaches that rely mainly on fractionating biomolecules under electrophoretic current based mainly on the molecular weight. In this book, the authors try to present simplified fundamentals of gel-based separation together with exemplarily applications of this versatile technique. We try to keep the contents of the book crisp and comprehensive, and hope that it will receive overwhelming interest and deliver benefits and valuable information to the readers. |
| coomassie brilliant blue r 250 staining solution: Tau Protein Caroline Smet-Nocca, |
| coomassie brilliant blue r 250 staining solution: Extraction Strategies to Recover Bioactive Compounds, Incorporation into Food and Health Benefits María del Mar Contreras, Eulogio Castro, 2020-05-22 We are pleased to present this book, which is a reprint of articles from the Special Issue entitled “Extraction Strategies to Recover Bioactive Compounds, Incorporation into Food, and Health Benefits” published online in the open access journal Foods (ISSN 2304-8158) from 2019 to 2020 (available at: https://www.mdpi.com/journal/foods/special_issues/extraction). Firstly, this book gathers studies addressing several strategies applied to obtain bioactive products and extracts, not only from food matrices but also from agri-food byproducts, which can serve as new natural additives, nutraceuticals, and functional ingredients for pharmaceutical, cosmetics, and food industries. In particular, free and bound phenolic compounds are explored in buckwheat, sesame, and olive leafy byproducts. Overall, these studies outline new valorization methods and offer new opportunities for alternative practices in the agro-industrial sector that help to migrate toward a circular bioeconomy model. This book also presents studies that predict bioactive components in fruits through mathematical tools and support the formulation of a novel beverage rich in resveratrol, a phenolic compound whose bioactivity is well recognized. |
| coomassie brilliant blue r 250 staining solution: Electrophoresis in Practice Reiner Westermeier, 2006-03-06 This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in use. This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references. |
| coomassie brilliant blue r 250 staining solution: Cerebral Cortex Development Koh-ichi Nagata, |
| coomassie brilliant blue r 250 staining solution: Hairy Root Cultures Based Applications Vikas Srivastava, Shakti Mehrotra, Sonal Mishra, 2020-06-27 The book discusses the various methods and protocols available in hairy root culture-based research. The utilization of Agrobacterium mediated genetic transformation and establishment of hairy root cultures has paved the way for large-scale secondary metabolite production in medicinal plants. Presenting recent research and offering insights from eminent research groups, the book covers a range of topics related to hairy root-based applications, including (i) establishment of hairy roots and native production of SM (ii) yield enhancement strategies for increased SM production, like elicitation (iii) hairy roots as a tool for value-added applications such as plant-microbe interaction, characterization of plant genes and root biology studies. As such it is an informative guide and experimental manual for researchers in diverse fields of plant biology. |
| coomassie brilliant blue r 250 staining solution: Food Analysis Laboratory Manual S. Suzanne Nielsen, 2017-06-07 This third edition laboratory manual was written to accompany Food Analysis, Fifth Edition, by the same author. New to this third edition of the laboratory manual are four introductory chapters that complement both the textbook chapters and the laboratory exercises. The 24 laboratory exercises in the manual cover 21 of the 35 chapters in the textbook. Many of the laboratory exercises have multiple sections to cover several methods of analysis for a particular food component or characteristic. Most of the laboratory exercises include the following: background, reading assignment, objective, principle of method, chemicals, reagents, precautions and waste disposal, supplies, equipment, procedure, data and calculations, questions, and references. This laboratory manual is ideal for the laboratory portion of undergraduate courses in food analysis. |
| coomassie brilliant blue r 250 staining solution: Protein Purification Jan-Christer Janson, Lars Rydén, 1998 This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis. |
| coomassie brilliant blue r 250 staining solution: Lee and Gaensslen's Advances in Fingerprint Technology Robert Ramotowski, 2012-10-18 Reflecting new discoveries in fingerprint science, Lee and Gaensslen‘s Advances in Fingerprint Technology, Third Edition has been completely updated with new material and nearly double the references contained in the previous edition. The book begins with a detailed review of current, widely used development techniques, as well as some older, histo |
| coomassie brilliant blue r 250 staining solution: Laboratory Methods in Enzymology: Protein Part C , 2014-03-22 In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on Protein |
| coomassie brilliant blue r 250 staining solution: Separation Methods Z. Deyl, 2011-09-22 Separation Methods |
| coomassie brilliant blue r 250 staining solution: The Science of Crime Scenes Max M. Houck, Frank Crispino, Terry McAdam, 2012-12-17 The Science of Crime Scenes addresses the science of crime scene investigation and processing, including the latest methods and technologies. This book covers the philosophy of crime scenes as historical events, the personnel involved at a scene (including the media), the detection of criminal traces and their reconstruction, and special crime scenes, such as mass disasters and terrorist events. Written by an international trio of authors with decades of crime scene experience, it is the next generation of crime scene textbooks. The book provides in-depth coverage of disasters and mass murder, terror crime scenes, and CBRN (chemical, biological, radioactive and nuclear) – topics not covered in any other text. It includes an instructor website with lecture slides, test bank, outlines, definitions, and activities, along with a student companion site with an image collection. This text will be of interest to advanced undergraduate and graduate students in forensic science programs, as well as to forensic practitioners and crime scene technicians. - Offers a science-based approach to crime scene investigation - Includes in-depth coverage of disasters and mass murder, terror crime scenes, and CBRN (chemical, biological, radioactive and nuclear) – not covered in any other text - Written by an international trio of authors with decades of crime scene experience - Instructor website with lecture slides, test bank, outlines, definitions, and activities, and a student companion site with an image collection |
| coomassie brilliant blue r 250 staining solution: Handbook of Food Analytical Chemistry, Volume 1 Ronald E. Wrolstad, Terry E. Acree, Eric A. Decker, Michael H. Penner, David S. Reid, Steven J. Schwartz, Charles F. Shoemaker, Denise M. Smith, Peter Sporns, 2005-09-02 Emphasizing effective, state-of-the art methodology and written by recognized experts in the field, the Handbook of Food Analytical Chemistry is an indispensable reference for food scientists and technologists to enable successful analysis. * Provides detailed reports on experimental procedures * Includes sections on background theory and troubleshooting * Emphasizes effective, state-of-the art methodology, written by recognized experts in the field * Includes detailed instructions with annotated advisory comments, key references with annotation, time considerations and anticipated results |
| coomassie brilliant blue r 250 staining solution: Structural Genomics and Drug Discovery Wayne F. Anderson, 2014-07-08 Structural Genomics and Drug Discovery: Methods and Protocols focuses on high throughput structure determination methods and how they can be applied to lay the groundwork for structure aided drug discovery. The methods and protocols that are described can be applied in any laboratory interested in using detailed structural information to advance the initial stages of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Structural Genomics and Drug Discovery: Methods and Protocols seeks to aid scientists in the further study into structural genomics approach as an efficient initial step toward drug discovery and the methods described will be useful to anyone interested in moving in this direction. |
| coomassie brilliant blue r 250 staining solution: Basic Plant Pathology Methods James B. Sinclair, Onkar Dev Dhingra, 2017-11-22 The Second Edition of this bestseller brings together basic plant pathology methods published in diverse and often abstract publications. The Second Edition is updated and expanded with numerous new figures, new culture media, and additional methods for working with a greater number of organisms. Methods are easy to use and eliminate the need to seek out original articles. This reference allows for easy identification of methods appropriate for specific problems and facilities. Scientific names of pathogens and some of their hosts are updated in this edition. The book also acts as a research source providing more than 1,800 literature citations. The Second Edition includes chapters on the following: Sterilization of culture apparatus and culture media Culture of pathogens with detailed techniques for 61 fungi and selected bacteria Long-term storage of plant pathogens Detection and estimation of inoculum for 28 soilborne fungal pathogens and 5 bacterial genera-15 methods for airborne inoculum and 13 methods for seedborne pathogens Establishment of disease and testing for disease resistance Work with soil microorganisms Fungicide evaluation Biological control Bright-field microscopy |
| coomassie brilliant blue r 250 staining solution: Proteoglycan Protocols Renato V. Iozzo, 2008-02-02 Proteoglycans are some of the most elaborate macromolecules of mammalian and lower organisms. The covalent attachment of at least five types of glycosami- glycan side chains to more than forty individual protein cores makes these molecules quite complex and endows them with a multitude of biological functions. Proteoglycan Protocols offers a comprehensive and up-to-date collection of prepa- tive and analytical methods for the in-depth analysis of proteoglycans. Featuring st- by-step detailed protocols, this book will enable both novice and experienced researchers to isolate intact proteoglycans from tissues and cultured cells, to establish the composition of their carbohydrate moieties, to generate strategies for prokaryotic and eukaryotic expression, to utilize methods for the suppression of specific proteoglycan gene expression and for the detection of mutant cells and degradation products, and to study specific interactions between proteoglycans and extracellular matrix proteins as well as growth factors and their receptors. The readers will find concise, yet comprehensive techniques carefully drafted by leading experts in the field. Each chapter commences with a general Introduction, followed by a detailed Materials section, and an easy-to-follow Methods section. An asset of each chapter is the extensive notation that includes troubleshooting tips and practical considerations that are often lacking in formal methodology papers. The reader will find this section most valuable because it is clearly provided by experienced scientists who have first-hand knowledge of the techniques they outline. In addition, most of the chapters are well illustrated with examples of typical data generated with each method. |
| coomassie brilliant blue r 250 staining solution: Autoimmunity Andras Perl, 2008-02-01 “Research is to see what everybody else has seen, and to think what nobody else has thought. ” — Albert Szentgyörgyi Autoimmunity: Methods and Protocols is intended to serve as a ready-to-use guide to establish and interrogate human and animal models of autoimmune diseases. The first chapter, “Pathogenesis and Spectrum of Autoimmunity,” discusses major hypotheses driving this most tantalizing area of research since Paul Ehrlich proposed the concept of autoimmunity in 1900. Considering the great diversity and ever-changing spectrum of autoimmunity, it has not been possible to include models and experimental protocols for each known disorder. Rather, several chapters have been devoted to the most prevalent and complex diseases, such as rheumatoid arthritis, systemic lupus erythematosus, insulin-dependent diabetes mellitus, and multiple sclerosis. The chapters are contributed by laboratories actively using the models presented. Each chapter contains an introductory section that discusses the relevance of the model for a particular disease and for autoimmunity in general. Part I contains methods and protocols to assess immunological and biochemical pathways relevant for disease pathogenesis. Chapters in this section focus on methods to identify susceptibility genes, intercellular signaling via cytokines, intracellular signaling through the T-cell receptor and signal processing via protein kinases, identification and enumeration of autoantigen-specific T cells and autoantibodies, and the dysregulation of apoptosis and its role in modification of self-antigens. Part II contains protocols to establish and assess inflammatory arthritis, systemic lupus erythematosus, myocarditis, thyroiditis, experimental autoimmune encepha- myelitis, insulin-dependent diabetes mellitus, scleroderma, uveitis, and vitiligo. |
| coomassie brilliant blue r 250 staining solution: URU International Conference on Science and Technology 2018 Ruangdet Wongla, 2019-01-04 Uttaradit Rajabhat University International Conference on Science and Technology 2018 (URUICST20018) Selected, peer reviewed papers from the URU International Conference on Science and Technology 2018 (URUICST 2018), August 2-3, 2018, Uttaradit, Thailand |
| coomassie brilliant blue r 250 staining solution: A Manual for Biochemistry Protocols Markus R. Wenk, Aaron Zefrin Fernandis, 2007 Biochemistry plays an important role in all areas of the biological and medical sciences. With most of the research or diagnosis involved in these areas being based on biochemically obtained observations, it is essential to have a profile of well standardized protocols. This manual is a basic guide for all students, researchers and experts in biochemistry, designed to help readers in directly starting off their experiments without prior knowledge of the protocol. The book dwells on the concepts used in designing the methodologies, thereby giving ample room for researchers to modify them according to their research requirements. |
| coomassie brilliant blue r 250 staining solution: Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics 8 E; South Asia Edition;e-Book Nader Rifai, 2019-07-16 Get the foundational knowledge you need to successfully work in a real-world, clinical lab with Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 8th Edition. From highly respected clinical chemistry expert Nader Rifai, this condensed, easier-to-understand version of the acclaimed Tietz Textbook of Clinical Chemistry and Molecular Diagnostics uses a laboratory perspective to guide you through selecting and performing diagnostic lab tests and accurately evaluating the results. Coverage includes laboratory principles, analytical techniques, instrumentation, analytes, pathophysiology, and more. This eighth edition features new clinical cases from The Coakley Collection, new questions from The Deacon's Challenge of Biochemical Calculations Collection, plus new content throughout the text to ensure you stay ahead of all the latest techniques, instrumentation, and technologies. Condensed version of the clinical chemistry bible offers the same authoritative and well-presented content in a much more focused and streamlined manner. Coverage of analytical techniques and instrumentation includes optical techniques, electrochemistry, electrophoresis, chromatography, mass spectrometry, enzymology, immunochemical techniques, microchips, automation, and point of care testing. Updated chapters on molecular diagnostics cover the principles of molecular biology, nucleic acid techniques and applications, and genomes and nucleic acid alterations, reflecting the changes in this rapidly evolving field. Learning objectives, key words, and review questions are included in each chapter to support learning. More than 500 illustrations plus easy-to-read tables help readers better understand and remember key concepts |
| coomassie brilliant blue r 250 staining solution: Antibody Engineering Volume 2 Roland E. Kontermann, Stefan Dübel, 2010-03-10 Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. Antibody Engineering provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest hands on knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development. |
| coomassie brilliant blue r 250 staining solution: Latent Print Processing Guide Stephen P. Kasper, 2015-12-28 Latent prints are chance or accidental impressions left by friction-ridge skin on a surface, regardless of whether they are visible or invisible at the time of deposition. Recognition of evidence that may contain fingerprints and the processes that can develop these latent prints is crucial in preventing valuable evidence from being left undetected. Latent Print Processing Guide goes beyond the basic police training, covering latent prints in detail and providing first responders with adequate training and guidelines. To process latent prints, examiners use various techniques including electronic, chemical, cyanoacrylate, and physical methods. Latent Print Processing Guide offers a broad understanding of latent print detection, development, and recovery, including insights on stateof-the-art technologies. - Includes history of latent print identification and some of the pioneers and their contributions. Defines the differences between chemical and physical processes and explains process sequence protocols and recovery methods for different types of evidence. - Chapters include: process selection, application and recovery, special considerations for specific materials, protocol sequence and process formulas, including required materials, application method, expected results, safety measures, and references. - The text is written so that non-crime scene or non-crime laboratory personnel can also gain valuable information from it. |
| coomassie brilliant blue r 250 staining solution: The Protein Protocols Handbook John M. Walker, 2007-10-02 The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry. |
| coomassie brilliant blue r-250 staining solution: Guide to Protein Purification Murray P. Deutscher, 1990 Guide to Protein Purification , designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features* Detailed procedures newly written for this volume* Extensive practical information* Rationale and strategies for protein and enzyme purification* Personal perspectives on enzyme purification by eminent researchersAmong the Topics Covered* General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure. |
| coomassie brilliant blue r-250 staining solution: Protein Gel Detection and Imaging Biji T. Kurien, R. Hal Scofield, 2019-08-24 This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Protein Gel Detection and Imaging: Methods and Protocols is a valuable resource for expert and novice scientists and researchers who are interested in learning and experimenting with this field. |
| coomassie brilliant blue r-250 staining solution: Western Blotting for the Non-Expert Biji T. Kurien, 2021-07-24 This book fills the need for a simplified text covering western blotting protocols aimed not just at high school and college students, but the researcher with little to no experience in these techniques. It provides the principles, basic methodology, and tips and tricks to avoiding the common pitfalls of western blotting. The book also introduces simple protocols that can transform western blotting into a fun method, such as sending secret messages on membranes or using nitrocellulose membrane as a canvas for art. In addition to the techniques, this book also covers the history of western blotting, which originated from the development of the blotting of DNA. It then delves into the importance of protein blotting, brought to the fore by the fact that the procedure has been evolving constantly since its inception in 1979, and the fact that the scientific community is faced with a multitude of ways and means of transferring proteins to membranes.. |
| coomassie brilliant blue r-250 staining solution: Protein Structure Analysis Roza Maria Kamp, Theodora Choli-Papadopoulou, Brigitte Wittmann-Liebold, 2012-12-06 Protein Structure Analysis - Preparation and Characterization is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on Microsequence Analysis of Proteins held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered. |
| coomassie brilliant blue r-250 staining solution: Techniques of Sample Preraration for Liquid Scintillation Counting+Isoelectric Focusing , 1976-01-01 Techniques of Sample Preraration for Liquid Scintillation Counting+Isoelectric Focusing |
| coomassie brilliant blue r-250 staining solution: Protein Electrophoresis Biji T. Kurien, R. Hal Scofield, 2012-05-16 Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field. |
| coomassie brilliant blue r-250 staining solution: Guide to Protein Purification Richard R Burgess, Murray P. Deutscher, 2009-11-03 Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This Guide to gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. - Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology - Assembles chapters on both common and less common relevant techniques - Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process |
| coomassie brilliant blue r-250 staining solution: Electrophoresis Budin Michov, 2020-09-07 Electrophoresis is a straightforward but informative analytical method used in biochemistry, biology and medicine. This book combines a detailed discussion of theory and technical application with an elaborate section on troubleshooting and problem solving in electrophoresis. Therefore the book is an important guide for both students and scientists. |
| coomassie brilliant blue r-250 staining solution: The Proteome Revisited A. Stoyanov, M. Zhukov, Pier Giorgio Righetti, 2001-10-25 The book deals with the theory and practice of all electrophoretic steps leading to proteome analysis, i.e. isoelectric focusing (including immobilized pH gradients), sodium dodecyl sulphate electrophoresis (SADS-PAGE) and finally two-dimensional maps. It is a reasoned collection of all modern, relevant, up-to-date methodologies leading to successful fractionation, analysis and characterization of every polypeptide spot in 2-D map analysis. It includes chapters on the most sophisticated mass spectrometry developments and it helps the reader in navigating through the most important databases in proteome analysis, including step by step tours in selected sites. Yet, this book's unique strength and feature is the fact that it combines not only practice (in common with any other book on this topic) but also theory, by giving a detailed treatment on the most advanced theoretical treatments of steady-state techniques, such as isoelectric focusing and immobilized pH gradients. A lot of this theory is newly developed and presented to the public for the first time. Thus, this book should satisfy not only the needs of every day practitioners, but also the desires of the most advanced theoreticians in the field, who will surely appreciate the novel theories presented here. Also the methodological section contains several as yet unpublished protocols, correcting some of the existing ones and showing the pitfall and limitations of even well ingrained protocols in proteome analysis, which are here critically re-evaluated for the first time. |
| coomassie brilliant blue r-250 staining solution: Introduction to Biophysical Methods for Protein and Nucleic Acid Research Jay A. Glasel, Murray P. Deutscher, 1995-11-20 The first of its kind, Introduction to Biophysical Methods for Protein and Nucleic Acid Research serves as a text for the experienced researcher and student requiring an introduction to the field. Each chapter presents a description of the physical basis of the method, the type of information that may be obtained with the method, how data should be analyzed and interpreted and, where appropriate, practical tips about procedures and equipment.Key Features* Modern Use of Mass Spectroscopy* NMR Spectroscopy* Molecular Modeling and Graphics* Macintosh and DOS/Windows 3.x disks |
| coomassie brilliant blue r-250 staining solution: Proteomics Today Mahmoud H. Hamdan, Pier G. Righetti, 2005-05-13 The last few years have seen an unprecedented drive toward theapplication of proteomics to resolving challenging biomedical andbiochemical tasks. Separation techniques combined with modern massspectrometry are playing a central role in this drive. This bookdiscusses the increasingly important role of mass spectrometry inproteomic research, and emphasizes recent advances in the existingtechnology and describes the advantages and pitfalls as well. * Provides a scientifically valid method for analyzing theapproximatey 500,000 proteins that are encoded in the humangenome * Explains the hows and whys of using mass spectrometry inproteomic analysis * Brings together the latest approaches combining separationtechniques and mass spectrometry and their application in proteomeanalysis * Comments on future challenges and how they may be addressed * Includes sections on troubleshooting |
| coomassie brilliant blue r-250 staining solution: Gel Electrophoresis and Isoelectric Focusing of Proteins Robert C. Allen, Calvin A. Saravis, H.R. Maurer, 2019-05-20 No detailed description available for Gel Electrophoresis and Isoelectric Focusing of Proteins. |
| coomassie brilliant blue r-250 staining solution: Electrophoresis Fundamentals Budin Michov, 2022-04-04 Electrophoresis methods are simple and informative analytical methods, which are used in biochemistry and medicine. They are necessary for precise investigation of proteins and nucleic acids (DNA and RNA). Electrophoresis is practiced in chemical, biochemical, and clinical laboratories, as well as in forensic medicine laboratories. This book will discuss the fundamentals of electrophoresis methods at the graduate level. |
| coomassie brilliant blue r-250 staining solution: Protides of the Biological Fluids H. Peeters, 2014-11-26 An International Review Series Devoted to Proteins and Related Studies, Volume 33: Protides of the Biological Fluids documents the proceedings of the 33rd Colloquium held in 1985. This book is divided into three sections. Section A focuses on the plasma protein genes that includes the location of genes on the chromosome, pathology and variance of several genes, and gene expression. The proteins of the nucleus and detection of antibodies, such as antinuclear antibodies (ANA), to nuclear constituents are deliberated in Section B. Section C is devoted to the technical advances in the field of protein chemistry. This book is a good reference for students, researchers, and medical practitioners conducting work on the protides of biological fluids. |
| coomassie brilliant blue r-250 staining solution: Epidermal Cells Kursad Turksen, 2008-02-03 A collection of cutting-edge methods to analyze and manipulate epidermal cellprecursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, and for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. These readily reproducible techniques broaden our understanding of the biology of epidermal cells and of their utility in normal tissue homeostasis and regenerative medicine applications. |
| coomassie brilliant blue r-250 staining solution: Gel Electrophoresis Sameh Magdeldin, 2012-04-04 Most will agree that gel electrophoresis is one of the basic pillars of molecular biology. This coined terminology covers a myriad of gel-based separation approaches that rely mainly on fractionating biomolecules under electrophoretic current based mainly on the molecular weight. In this book, the authors try to present simplified fundamentals of gel-based separation together with exemplarily applications of this versatile technique. We try to keep the contents of the book crisp and comprehensive, and hope that it will receive overwhelming interest and deliver benefits and valuable information to the readers. |
| coomassie brilliant blue r-250 staining solution: Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems , 2020-05-14 Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on retinoid signaling pathways |
| coomassie brilliant blue r-250 staining solution: Microneedles for Transdermal Drug Delivery Jaspreet Singh Kochhar, Justin J. Y. Tan, Yee Chin Kwang, Lifeng Kang, 2019-07-04 This monograph covers a novel technology to deliver drugs and cosmetics through the skin in a minimally invasive manner. Microneedles – a bed of miniaturized needles is one of the most studied topics in delivering actives through the skin barrier. This book enables readers to understand the delivery of ingredients through the skin, describes a novel and simple method to fabricate microneedles containing a range of small and large molecular weight compounds, studies their physical properties as well as delivery through the skin layers. Readers will discover this book to be extremely beneficial to help them understand the state of the field of transdermal drug delivery, with extensive coverage including experimental data on basics of microneedle fabrication technology using photolithography, encapsulation of drugs within the polymeric matrix of microneedles and studying their release patternsin vitro and ex vivo . Academic researchers, pharmaceutical and cosmeceutical industry as well as students of skin science will find this account very useful in their pursuits. As microneedles grow and develop into a commercial reality with more actives being delivered and significant clinical research being put in, this account will hold well in providing basic principles and knowledge together with rigorous experimental data. |
| coomassie brilliant blue r-250 staining solution: DNA Fingerprinting: Advancements and Future Endeavors Hirak Ranjan Dash, Pankaj Shrivastava, Braja Kishore Mohapatra, Surajit Das, 2018-11-01 This book describes the basics and various applications of DNA fingerprinting, including in actual case studies. The book is divided in four modules; Module 1: Basics of DNA Fingerprinting, Module 2: Applications of DNA Fingerprinting, Module 3: DNA Fingerprinting: Case Studies, and Module 4: Future of DNA Fingerprinting. Each module consists of 4 to 5 chapters, written by reputed researchers, academics and forensic scientists from around the globe. The respective chapters cover e.g. related fields, the tools and techniques used, various genotyping kits, real-world case studies, ancient DNA and wild life forensics, molecular diagnosis of human diseases, legal aspects, microbial forensics and the economics of the DNA fingerprinting technique. The book offers a practical guide for professionals, graduate and post-graduate students in the fields of Forensic Science, Medicine, Genetics, Anthropology, Microbiology, and Zoology. It also serves as a useful reference resource, summarizing major technological advances in the field of DNA fingerprinting, the problems faced in this field of science and possible new solutions to these problems. Presently, DNA fingerprinting is utilized in solving the majority of criminal cases; as such, the book is also helpful for investigating agencies, as it includes representative case studies. |
| coomassie brilliant blue r-250 staining solution: Lee and Gaensslen's Advances in Fingerprint Technology Allan Gaw, 2012-10-18 Reflecting new discoveries in fingerprint science, Lee and Gaensslen‘s Advances in Fingerprint Technology, Third Edition has been completely updated with new material and nearly double the references contained in the previous edition. The book begins with a detailed review of current, widely used development techniques, as well as some older, histo |
| coomassie brilliant blue r-250 staining solution: Chemical Tools in Microbiology 1 , 2022-03-22 Clinical Microbiology, Volume 664 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Synthesis of chemical probes to study bacterial adenylating enzymes, Fluoroquinolone-derived fluorescent probes for studies of bacterial penetration and efflux, Combining informatics with ABPP to identify serine hydrolyses in bacteria, A Ligand Selection Strategy Identifies Chemical Probes Targeting the Proteases of SARS-CoV-2, Activity-based probes for bacterial histidine kinases, Metabolomic approaches to enzyme function and pathway discovery, Identification of bile salt hydrolase activity in gut microbiota, and much more. Other chapters cover Multiplex fluorescence screening and identification using multiplex TMT, Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via SPR, Site-Specific Siderocalin Binding to Ferric and Ferric-Free Enterobactin As Revealed by Mass Spectrometry, Proteomics of short-chain fatty acid probes in Salmonella, Development and application of highly sensitive labeling reagents for amino acids, and a variety of other timely topics. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology serials - Updated release includes the latest information on Chemical Microbiology |
| coomassie brilliant blue r-250 staining solution: Essentials of Biotechnology Ulhas K. Patil, Kalyani Muskan, 2009 Essentials of Biotechnology is meant for undergraduate biotechnology and life sciences students. The book discusses the basics of interdisciplinary subjects which is required for developing the conceptual understanding in biotechnology and to acquire research attitude. It elaborates fundamental concepts which are absolutely necessary for budding biotechnologists. It is an attempt to cover broad spectrum of biological dimensions with biotechnological exploration. Section-I elaborates theoretical aspects of basic biology, biochemistry, microbiology, molecular biology with correlation to modern applied aspects.Section-II is grounded in the experimental approach. Each experiment is described with sufficient details. The figures and tables provided with experiments will be helpful to the students and the instructor for better understanding of the scientific principles and skillful execution of the experiments. |
| coomassie brilliant blue r-250 staining solution: Electrophoresis in Practice Reiner Westermeier, 2006-03-06 This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in use. This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references. |
| coomassie brilliant blue r-250 staining solution: Bacterial Pathogenesis, Part C: Identification, Regulation and Function of Virulence Factors , 2002-12-05 The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.Key Features* Presents alternatives to mammalian model systems* Discusses virulence and essential gene identification* Defines global gene expression |
| coomassie brilliant blue r-250 staining solution: Plant Biotechnology and Molecular Biology : A Laboratory Manual M.S. Punia, 2018-01-01 The book, “A Laboratory Manual of Plant Biotechnology and Molecular Biology” comprises of workable laboratory protocols for a large number of techniques related to plant biotechnology, genetic engineering and molecular biology. This includes plant cell and tissue culture, callus and suspension culture, anther culture, ovule culture, embryo culture, Cryopreservation, Isolation of Plant protoplasts, Protoplast culture and regeneration, production of somatic hybrids through protoplast fusion, gene transformation using Agrobacterium as vector, direct gene transfer using biolistic gun, Isolation of plant and organells DNA, construction and screening of genomic DNA libraries, Molecular markers like RFLP, RAPD, SCARS and CAPS, DNA sequencing, RNA isolation and northern blotting, Isolation of proteins and western blotting etc. The manual is prepared with the objective to cater the needs of post- graduate students as well as for scientists working in the disciplines of Plant Breeding, Genetics, Botany, Plant physiology, Biochemistry, Plant Biotechnology, Molecular Biology etc. It gives an update on some well established methods and presents reliable protocols. |
| coomassie brilliant blue r-250 staining solution: A Manual for Biochemistry Protocols Markus R. Wenk, Aaron Zefrin Fernandis, 2007 Biochemistry plays an important role in all areas of the biological and medical sciences. With most of the research or diagnosis involved in these areas being based on biochemically obtained observations, it is essential to have a profile of well standardized protocols. This manual is a basic guide for all students, researchers and experts in biochemistry, designed to help readers in directly starting off their experiments without prior knowledge of the protocol. The book dwells on the concepts used in designing the methodologies, thereby giving ample room for researchers to modify them according to their research requirements. |
| coomassie brilliant blue r-250 staining solution: Extraction Strategies to Recover Bioactive Compounds, Incorporation into Food and Health Benefits María del Mar Contreras, Eulogio Castro, 2020-05-22 We are pleased to present this book, which is a reprint of articles from the Special Issue entitled “Extraction Strategies to Recover Bioactive Compounds, Incorporation into Food, and Health Benefits” published online in the open access journal Foods (ISSN 2304-8158) from 2019 to 2020 (available at: https://www.mdpi.com/journal/foods/special_issues/extraction). Firstly, this book gathers studies addressing several strategies applied to obtain bioactive products and extracts, not only from food matrices but also from agri-food byproducts, which can serve as new natural additives, nutraceuticals, and functional ingredients for pharmaceutical, cosmetics, and food industries. In particular, free and bound phenolic compounds are explored in buckwheat, sesame, and olive leafy byproducts. Overall, these studies outline new valorization methods and offer new opportunities for alternative practices in the agro-industrial sector that help to migrate toward a circular bioeconomy model. This book also presents studies that predict bioactive components in fruits through mathematical tools and support the formulation of a novel beverage rich in resveratrol, a phenolic compound whose bioactivity is well recognized. |
| coomassie brilliant blue r-250 staining solution: Tau Protein Caroline Smet-Nocca, |
| coomassie brilliant blue r-250 staining solution: Cerebral Cortex Development Koh-ichi Nagata, |
| coomassie brilliant blue r-250 staining solution: Hairy Root Cultures Based Applications Vikas Srivastava, Shakti Mehrotra, Sonal Mishra, 2020-06-27 The book discusses the various methods and protocols available in hairy root culture-based research. The utilization of Agrobacterium mediated genetic transformation and establishment of hairy root cultures has paved the way for large-scale secondary metabolite production in medicinal plants. Presenting recent research and offering insights from eminent research groups, the book covers a range of topics related to hairy root-based applications, including (i) establishment of hairy roots and native production of SM (ii) yield enhancement strategies for increased SM production, like elicitation (iii) hairy roots as a tool for value-added applications such as plant-microbe interaction, characterization of plant genes and root biology studies. As such it is an informative guide and experimental manual for researchers in diverse fields of plant biology. |
| coomassie brilliant blue r-250 staining solution: Food Analysis Laboratory Manual S. Suzanne Nielsen, 2017-06-07 This third edition laboratory manual was written to accompany Food Analysis, Fifth Edition, by the same author. New to this third edition of the laboratory manual are four introductory chapters that complement both the textbook chapters and the laboratory exercises. The 24 laboratory exercises in the manual cover 21 of the 35 chapters in the textbook. Many of the laboratory exercises have multiple sections to cover several methods of analysis for a particular food component or characteristic. Most of the laboratory exercises include the following: background, reading assignment, objective, principle of method, chemicals, reagents, precautions and waste disposal, supplies, equipment, procedure, data and calculations, questions, and references. This laboratory manual is ideal for the laboratory portion of undergraduate courses in food analysis. |
| coomassie brilliant blue r-250 staining solution: Latent Print Processing Guide Stephen P. Kasper, 2015-12-28 Latent prints are chance or accidental impressions left by friction-ridge skin on a surface, regardless of whether they are visible or invisible at the time of deposition. Recognition of evidence that may contain fingerprints and the processes that can develop these latent prints is crucial in preventing valuable evidence from being left undetected. Latent Print Processing Guide goes beyond the basic police training, covering latent prints in detail and providing first responders with adequate training and guidelines. To process latent prints, examiners use various techniques including electronic, chemical, cyanoacrylate, and physical methods. Latent Print Processing Guide offers a broad understanding of latent print detection, development, and recovery, including insights on stateof-the-art technologies. - Includes history of latent print identification and some of the pioneers and their contributions. Defines the differences between chemical and physical processes and explains process sequence protocols and recovery methods for different types of evidence. - Chapters include: process selection, application and recovery, special considerations for specific materials, protocol sequence and process formulas, including required materials, application method, expected results, safety measures, and references. - The text is written so that non-crime scene or non-crime laboratory personnel can also gain valuable information from it. |
| coomassie brilliant blue r-250 staining solution: Protein Purification Jan-Christer Janson, Lars Rydén, 1998 This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis. |
| coomassie brilliant blue r-250 staining solution: Trophoblasts Sandeep Raha, 2023-12-31 This volume explores the latest approaches used to assess trophoblast angiogenesis, transport function, cellular respirations, migration, and invasion. The chapters in this book cover topics such as various methods to study and manipulate primary trophoblast cells; strategies using liposomes to deliver biomolecules to trophoblasts; in vitro models of the placenta that emulate the cellular interactions in the 3D uterine environment; and models exploring heterogenous cell types and 3D-organoid structures that represent cell-to-cell interactions in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Trophoblasts: Methods and Protocols is a valuable resource that will help researchers better understand the function of the placenta and its complex contribution to maternal health and fetal development, and also provide greater insights to the area of study known as the developmental origins of health and diseases (DoHaD). |
| coomassie brilliant blue r-250 staining solution: Antibiotics Peter Sass, 2022-11-29 This second edition provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, as well as methods for the analysis and detection of microbial responses and adaptation strategies. Antibiotics: Methods and Protocols, Second Edition, guides readers through updated and entirely new chapters on production and design, mode of action, and response and resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Antibiotics: Methods and Protocols, Second Edition aims to inspire scientific work in the exciting field of antibiotic research. |
| coomassie brilliant blue r-250 staining solution: Laboratory Methods in Enzymology: Protein Part C , 2014-03-22 In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on Protein |
| coomassie brilliant blue r-250 staining solution: The Maillard Reaction S. E. Fayle, Juliet A. Gerrard, 2002 It is almost 100 years since the Maillard reaction was first described. Despite decades of research since then, the products of the reaction and the mechanistic pathways leading to their formation are only gradually being unravelled. Combining comprehensive information regarding the various methods that can be employed in the analysis of Maillard products with a discussion of the advantages and limitations of those methods, this unique book provides a 'one-stop' text from which methods of analysis may be obtained. It will be useful for both new and experienced researchers who are involved in solving the mysteries and complexities of Maillard chemistry. |
| coomassie brilliant blue r-250 staining solution: Structural Genomics and Drug Discovery Wayne F. Anderson, 2014-07-08 Structural Genomics and Drug Discovery: Methods and Protocols focuses on high throughput structure determination methods and how they can be applied to lay the groundwork for structure aided drug discovery. The methods and protocols that are described can be applied in any laboratory interested in using detailed structural information to advance the initial stages of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Structural Genomics and Drug Discovery: Methods and Protocols seeks to aid scientists in the further study into structural genomics approach as an efficient initial step toward drug discovery and the methods described will be useful to anyone interested in moving in this direction. |
| coomassie brilliant blue r-250 staining solution: Separation Methods Z. Deyl, 2011-09-22 Separation Methods |
| coomassie brilliant blue r-250 staining solution: Basic Plant Pathology Methods James B. Sinclair, Onkar Dev Dhingra, 2017-11-22 The Second Edition of this bestseller brings together basic plant pathology methods published in diverse and often abstract publications. The Second Edition is updated and expanded with numerous new figures, new culture media, and additional methods for working with a greater number of organisms. Methods are easy to use and eliminate the need to seek out original articles. This reference allows for easy identification of methods appropriate for specific problems and facilities. Scientific names of pathogens and some of their hosts are updated in this edition. The book also acts as a research source providing more than 1,800 literature citations. The Second Edition includes chapters on the following: Sterilization of culture apparatus and culture media Culture of pathogens with detailed techniques for 61 fungi and selected bacteria Long-term storage of plant pathogens Detection and estimation of inoculum for 28 soilborne fungal pathogens and 5 bacterial genera-15 methods for airborne inoculum and 13 methods for seedborne pathogens Establishment of disease and testing for disease resistance Work with soil microorganisms Fungicide evaluation Biological control Bright-field microscopy |
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