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| cell cycle analysis protocol: Flow Cytometry Protocols Teresa S. Hawley, |
| cell cycle analysis protocol: Current Protocols on CD-ROM. , 1997 |
| cell cycle analysis protocol: Checkpoint Controls and Cancer Axel H. Schönthal, 2004-06-24 Intracellular checkpoint controls constitute a network of signal transd- tion pathways that protect cells from external stresses and internal errors. Ext- nal stresses can be generated by the continuous assault of DNA-damaging agents, such as environmental mutagens, ultraviolet (UV) light, ionizing radiation, or the reactive oxygen species that can arise during normal cellular metabolism. In response to any of these assaults on the integrity of the genome, the activation of the network of checkpoint control pathways can lead to diverse cellular responses, such as cell cycle arrest, DNA repair, or elimination of the cell by cell death (apoptosis) if the damage cannot be repaired. Moreover, internal errors can occur during the highly orchestrated replication of the cellular genome and its distribution into daughter cells. Here, the temporal order of these cell cycle events must be strictly enforced—for example, to ensure that DNA replication is c- plete and occurs only once before cell division, or to monitor mitotic spindle assembly, and to prevent exit from mitosis until chromosome segregation has been completed. Thus, well functioning checkpoint mechanisms are central to the maintenance of genomic integrity and the basic viability of cells and, the- fore, are essential for proper development and survival. The importance of proper functioning of checkpoints becomes plainly obvious under conditions in which this control network malfunctions and fails. Depending on the severity and timing, failure of this machinery can lead to embryonic lethality, genetic diseases, and cancer. |
| cell cycle analysis protocol: Cellular Quiescence H. Daniel Lacorazza, 2017-10-18 This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative medicine in the future. The chapters in this book were designed to address cellular quiescence in prokaryote and eukaryote organisms, detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU, H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal, neuronal, hematopoietic), genomic regulation (gene expression, transcription factors, lncRNA, RNA methylation), as well as analysis of the heterogeneity of quiescence by computer modeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cellular Quiescence: Methods and Protocols offers a broad view of basic and cutting-edge technology to inspire research in this emerging field of cell biology. |
| cell cycle analysis protocol: Cell Cycle Oscillators Amanda S. Coutts, Louise Weston, 2015-08-09 This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest developments in molecular biology techniques, biochemistry, and computational analysis used for studying oscillatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell Cycle Oscillators: Methods and Protocols will serve as an invaluable reference to gain further insight into the complex and incompletely understood processes that are involved in the cell cycle and its regulation by oscillatory networks. |
| cell cycle analysis protocol: Cell Cycle Checkpoint Control Protocols Howard B. Lieberman, 2003-11-14 The field of cell cycle regulation is based on the observation that the life cycle of a cell progresses through several distinct phases, G1, M, S, and G2, occurring in a well-defined temporal order. Details of the mechanisms involved are rapidly emerging and appear extraordinarily complex. Furthermore, not only is the order of the phases important, but in normal eukaryotic cells one phase will not begin unless the prior phase is completed successfully. Che- point control mechanisms are essentially surveillance systems that monitor the events in each phase, and assure that the cell does not progress prematurely to the next phase. If conditions are such that the cell is not ready to progress—for example, because of incomplete DNA replication in S or DNA damage that may interfere with chromosome segregation in M—a transient delay in cell cycle progression will occur. Once the inducing event is properly handled— for example, DNA replication is no longer blocked or damaged DNA is repaired—cell cycle progression continues. Checkpoint controls have recently been the focus of intense study by investigators interested in mechanisms that regulate the cell cycle. Furthermore, the relationship between checkpoint c- trol and carcinogenesis has additionally enhanced interest in these cell cycle regulatory pathways. It is clear that cancer cells often lack these checkpoints and exhibit genomic instability as a result. Moreover, several tumor suppressor genes participate in checkpoint control, and alterations in these genes are as- ciated with genomic instability as well as the development of cancer. |
| cell cycle analysis protocol: Flow Cytometry and Cell Sorting Andreas Radbruch, 2013-03-14 The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended. CYTOBIOS |
| cell cycle analysis protocol: Cancer Cell Culture Simon P. Langdon, 2008-02-01 The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Methods to modify cancer cells are described in Part V, including protocols for transfection, development of drug-resistance, immortalization, and transfer in vivo. In Part VI methods of coculture of different cell types and contamination of cell lines are covered. |
| cell cycle analysis protocol: Flow Cytometry Alice Longobardi Givan, 2013-04-10 Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature. |
| cell cycle analysis protocol: Practical Flow Cytometry Howard M. Shapiro, 2005-02-25 From the reviews of the 3rd Edition... The standard reference for anyone interested in understandingflow cytometry technology. American Journal of Clinical Oncology ...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating. Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers. |
| cell cycle analysis protocol: Techniques in Cell Cycle Analysis Joe W. Gray, Zbigniew Darzynkiewicz, 2008-02-24 Quantification of the proliferative characteristics of normal and malignant cells has been of interest to oncolo gists and cancer biologists for almost three decades. This interest stems from (a) the fact that cancer is a disease of uncontrolled proliferation, (b) the finding that many of the commonly used anticancer agents are preferentially toxic to cells that are actively proliferating, and (c) the observa tion that significant differences in proliferation characteristics exist between normal and malignant cells. Initially, cell cycle analysis was pursued enthusiastically in the hope of gener ating information useful for the development of rational cancer therapy strategies; for example, by allowing identi fication of rapidly proliferating tumors against which cell cycle-specific agents could be used with maximum effec tiveness and by allowing rational scheduling of cell cyc- specific therapeutic agents to maximize the therapeutic ratio. Unfortunately, several difficulties have prevented realiza tion of the early promise of cell cycle analysis: Proliferative patterns of the normal and malignant tissues have been found to be substantially more complex than originally an ticipated, and synchronization of human tumors has proved remarkably difficult. Human tumors of the same type have proved highly variable, and the cytokinetic tools available for cell cycle analysis have been labor intensive, as well as somewhat subjective and in many cases inapplicable to humans. However, the potential for substantially improved cancer therapy remains if more accurate cytokinetic infor mation about human malignancies and normal tissues can be obtained in a timely fashion. |
| cell cycle analysis protocol: Cell Cycle Control Eishi Noguchi, Mariana C. Gadaleta, 2016-08-23 A collection of new reviews and protocols from leading experts in cell cycle regulation, Cell Cycle Control: Mechanisms and Protocols, Second Edition presents a comprehensive guide to recent technical and theoretical advancements in the field. Beginning with the overviews of various cell cycle regulations, this title presents the most current protocols and state-of-the-art techniques used to generate latest findings in cell cycle regulation, such as protocols to analyze cell cycle events and molecules. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cell Cycle Control: Mechanisms and Protocols, Second Edition will be a valuable resource for a wide audience, ranging from the experienced cell cycle researchers looking for new approaches to the junior graduate students giving their first steps in cell cycle research. |
| cell cycle analysis protocol: The Cell Cycle David Owen Morgan, 2007 The Cell Cycle: Principles of Control provides an engaging insight into the process of cell division, bringing to the student a much-needed synthesis of a subject entering a period of unprecedented growth as an understanding of the molecular mechanisms underlying cell division are revealed. |
| cell cycle analysis protocol: Flow Cytometry in Hematopathology Doyen T. Nguyen, Lawrence W. Diamond, Raul C. Braylan, 2003 This unique text offers a systematic and practical approach to the analysis and interpretation of FCM graphics. Using numerous FCM illustrations derived from actual well-documented clinical cases, the authors demonstrate a step-by-step approach to optimal FCM data analysis on specimens suspected of harboring hematopoietic malignancies. The discussion moves from simple to complex specimens, with an emphasis on visual pattern analysis. A wide variety of hematologic disorders are covered, including leukemias and lymphomas. The companion CD-ROM with 80 detailed case studies provides additional opportunities to gain a deeper understanding of FCM data analysis. |
| cell cycle analysis protocol: Mass Cytometry Helen M McGuire, 2019-08-19 |
| cell cycle analysis protocol: Flow Cytometry M. G. Ormerod, David Novo, 2008 Flow cytometry is a technique used to study cells, such as blood cells or cancer cells. It is used in medical and research laboratories. |
| cell cycle analysis protocol: Molecular Biology of the Cell , 2002 |
| cell cycle analysis protocol: Cytotoxicity Erman Salih Istifli, Hasan Basri İla, 2019-10-02 Compensating for cytotoxicity in the multicellular organism by a certain level of cellular proliferation is the primary aim of homeostasis. In addition, the loss of cellular proliferation control (tumorigenesis) is at least as important as cytotoxicity, however, it is a contrasting trauma. With the disruption of the delicate balance between cytotoxicity and proliferation, confrontation with cancer can inevitably occur. This book presents important information pertaining to the molecular control of the mechanisms of cytotoxicity and cellular proliferation as they relate to cancer. It is designed for students and researchers studying cytotoxicity and its control. |
| cell cycle analysis protocol: Immunophenotyping J Philip McCoy Jr, 2019-09-29 This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field. |
| cell cycle analysis protocol: Cell Proliferation and Apoptosis David Hughes, Huseyin Mehmet, 2004-03 Cell Proliferation and Apoptosis provides a detailed practical guide to cell proliferation and apoptosis detection methods. A novel approach combining both these areas allows important comparisons to be made. Topics covered include all aspects of tissue handling from collection, storage, fixation and processing through to locating and quantifying cells in different stages of the cell cycle. This book is an essential and comprehensive practical guide to these important and expanding areas. |
| cell cycle analysis protocol: Cell-Cycle Synchronization Zhixiang Wang, 2022-08-31 This volume covers a broad range of cell types including cultured cell lines, primary cells, and various unicellular organisms such as fission yeast, budding yeast, parasite Leishmania amazonensis, and parasite Trypanosoma brucei. The chapters in this book are organized into four parts. Part One looks at a general overview of cell cycle control and synchronization. Part Two discusses techniques to synchronize mammalian cells to various cell cycle phases including mitotic sub-phases. Part Three covers synchronization of unicellular organisms and Part Four analyzes cell cycle progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cell-Cycle Synchronization: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field. |
| cell cycle analysis protocol: Flow Cytometry with Plant Cells Jaroslav Dolezel, Johann Greilhuber, Jan Suda, 2007-06-27 Targeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their genomes. Following a brief introduction that highlights general considerations when analyzing plant cells by flow cytometric methods, the book goes on to discuss examples of application in plant genetics, genomic analysis, cell cycle analysis, marine organism analysis and breeding studies. With its list of general reading and a glossary of terms, this first reference on FCM in plants fills a real gap by providing first-hand practical hints for the growing community of plant geneticists. |
| cell cycle analysis protocol: Neurocytology Ennio Pannese, 1994 In the vast field of neuroscience, the introduction over the last 30 years of new investigative techniques (such as transmission and scanning electron microscopy, freeze-fracturing technique, cell organelle isolation by differential centrifugation, autoradiography, tracing techniques and immunocytochemistry) has greatly expanded our knowledge of neurocytology. This new information, however, is generally dispersed in the specialist journals or collected in reviews on specific topics. As a result, those whose interests lie in neurocytology have difficulty not only in finding data relative to their particular research, but also and above all, in gaining an overall and systematic vision of their discipline. It was this situation which prompted Ennio Pannese to systematize the major acquisitions on the minute structure of nerve and neuroglial cells and their interrelationships, correlate them with the classical concepts of light microscopy and integrate them, where possible, with elements of biochemistry and cell physiology. |
| cell cycle analysis protocol: Fission Yeast Iain Hagan, Antony M. Carr, Agnes Grallert, Paul Nurse, 2016 Fission yeast are unicellular, rod-shaped fungi that divide by medial fission. Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. Their rapid growth rate, genetic malleability, and similarities to more complex eukaryotes continue to make them excellent subjects for many biochemical, molecular, and cell biological studies. This laboratory manual provides an authoritative collection of core experimental procedures that underpin modern fission yeast research. The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies for probing the cell cycle, technologies for assessing proteins, metabolites, and cell wall constituents, imaging methods to visualize subcellular structures and dynamics, and protocols for investigating chromatin and nucleic acid metabolism. Modifications to techniques commonly used in related species (e.g., budding yeast) are noted, as are useful resources for fission yeast researchers, including various databases and repositories. The well-studied fission yeast Schizosaccharomyces pombe is the focus throughout, but the emerging model S. japonicus-a larger, dimorphic species with several desirable characteristics-is also covered. This manual is an important reference for existing fission yeast laboratories and will serve as an essential start-up guide for those working with fission yeast for the first time. |
| cell cycle analysis protocol: Imaging Flow Cytometry Natasha S. Barteneva, Ivan A. Vorobjev, 2015-11-23 This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies. |
| cell cycle analysis protocol: Angiogenesis Assays Carolyn A. Staton, Claire Lewis, Roy Bicknell, 2007-01-11 Angiogenesis, the development of new blood vessels from the existing vasculature, is essential for physiological growth and over 18,000 research articles have been published describing the role of angiogenesis in over 70 different diseases, including cancer, diabetic retinopathy, rheumatoid arthritis and psoriasis. One of the most important technical challenges in such studies has been finding suitable methods for assessing the effects of regulators of eh angiogenic response. While increasing numbers of angiogenesis assays are being described both in vitro and in vivo, it is often still necessary to use a combination of assays to identify the cellular and molecular events in angiogenesis and the full range of effects of a given test protein. Although the endothelial cell - its migration, proliferation, differentiation and structural rearrangement - is central to the angiogenic process, it is not the only cell type involved. the supporting cells, the extracellular matrix and the circulating blood with its cellular and humoral components also contribute. In this book, experts in the use of a diverse range of assays outline key components of these and give a critical appraisal of their strengths and weaknesses. Examples include assays for the proliferation, migration and differentiation of endothelial cells in vitro, vessel outgrowth from organ cultures, assessment of endothelial and mural cell interactions, and such in vivo assays as the chick chorioallantoic membrane, zebrafish, corneal, chamber and tumour angiogenesis models. These are followed by a critical analysis of the biological end-points currently being used in clinical trials to assess the clinical efficacy of anti-angiogenic drugs, which leads into a discussion of the direction future studies should take. This valuable book is of interest to research scientists currently working on angiogenesis in both the academic community and in the biotechnology and pharmaceutical industries. Relevant disciplines include cell and molecular biology, oncology, cardiovascular research, biotechnology, pharmacology, pathology and physiology. |
| cell cycle analysis protocol: Development and Reproduction in Humans and Animal Model Species Werner A. Mueller, Monika Hassel, Maura Grealy, 2015-01-03 This book describes human development including sexual reproduction and stem cell research with the development of model organisms that are accessible to genetic and experimental analysis in readily understandable texts and 315 multi-colored graphics. The introductory account of model organisms selected from the entire animal kingdom presents general principles, which are then outlined in subsequent chapters devoted to, for example, sexual development; genes controlling development and their contemporary molecular-analysis methods; production of clones and transgenic animals; development of the nervous and circulatory systems; regenerative medicine and ageing. Finally the evolution of developmental toolkits and novelties is discussed including the genetic basis of the enlargement of the human forebrain. Separate boxes are devoted to controversial questions such as the benefits and problems of prenatal diagnostics or the construction of ancient body plans. |
| cell cycle analysis protocol: Protocol Handbook for Cancer Biology Gauri Misra, Jyotika Rajawat, 2021-02-12 Protocol Handbook for Cancer Biology brings together a comprehensive collection of the methods used for cancer assessment, diagnostics, and therapeutics. Various protocols are discussed along with alternative strategies, including the advantages and limitations of techniques that have been used in labs globally. These protocols are presented by cancer biology experts based on their real-world experience. The protocols in this book will be a valuable resource for cancer researchers and graduate students, who can utilize the techniques described to conduct research more efficiently and successfully. - Presents comprehensive protocols used for cancer assessment, diagnostics, and therapeutics all in one place - Encompasses alternative strategies considering the requirements of the end user and taking into consideration diverse research settings - Discusses limitations and advantages of each method in experimental design and execution, thus saving time during the research process |
| cell cycle analysis protocol: Cytometry, Part A , 2000-10-31 Each chapter presents a detailed background of the described method, its theoretical foundations, and its applicability to different biomedical material. Updated chapters describe either the most popular methods or those processes that have evolved the most since the past edition. Additionally, a large portion of the volume is devoted to clinical cytometry. Particular attention is paid to applications of cytometry in oncology, the most rapidly growing area. - Contains 56 extensive chapters authored by world authorities on cytometry - Covers a wide range of topics, including principles of cytometry and general methods, cell preparation, tandardization and quality assurance, cell proliferation, apoptosis, cell-cell/cell-environmental interactions, cytogenetics and molecular genetics, cell function and differentiation, experimental and clinical oncology, microorganisms, and infectious diseases - Describes in-depth the essential methods and scientific principles of flow and laser scanning cytometry and illustrates how they can be applied to the fields of biology and medicine - Complements the first and second editions on flow cytometry in the Methods in Cell Biology series and includes new sections on technology principles |
| cell cycle analysis protocol: Hi-C Data Analysis Silvio Bicciato, Francesco Ferrari, 2022-09-04 This volume details a comprehensive set of methods and tools for Hi-C data processing, analysis, and interpretation. Chapters cover applications of Hi-C to address a variety of biological problems, with a specific focus on state-of-the-art computational procedures adopted for the data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Hi-C Data Analysis: Methods and Protocols aims to help computational and molecular biologists working in the field of chromatin 3D architecture and transcription regulation. |
| cell cycle analysis protocol: Budding Yeast Brenda Andrews, Charles M. Boone, Trisha Davis, Stanley Fields, 2016 Over the past century, studies of the budding yeast Saccharomyces cerevisiae have helped to unravel principles of nearly every aspect of eukaryotic cell biologyfrom metabolism and molecular genetics to cell division and differentiation. Thanks to its short generation time, ease of genetic manipulation, and suitability for high-throughput studies, yeast remains the focus of research in a vast number of laboratories worldwide. This laboratory manual provides a comprehensive collection of experimental procedures that continue to make budding yeast an informative model. The contributors describe methods for culturing and genetically modifying yeast, strategies and tools (e.g., gene deletion collections) for functional analyses, approaches for characterizing cell structure and morphology, and techniques to probe the modifications and interactions of various cellular constituents (e.g., using one- and two-hybrid screens). Strategies for studying metabolomics, complex traits, and evolution in yeast are also covered, as are methods to isolate and investigate new strains of yeast from the wild. Several additional chapters are devoted to bioinformatics tools and resources for yeast biology (e.g., the Saccharomyces Genome Database). This manual is therefore an essential resource for all researchers, from graduate level upward, who use budding yeast to explore the intricate workings of cells. |
| cell cycle analysis protocol: Cancer Cell Culture Ian A. Cree, 2016-08-23 With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field. |
| cell cycle analysis protocol: Current Protocols in Molecular Biology , |
| cell cycle analysis protocol: Hello, Laura! Laura Ingalls Wilder, 1996 Spend a busy day with laura in her little house on the prairie! |
| cell cycle analysis protocol: DNA Recombination and Repair Paul James Smith, Christopher John Jones, 1999 The processes of DNA recombination and repair are vital to cell integrity - an error can lead to disease such as cancer. It is therefore a large and exciting area of research and is also taught on postgraduate and undergraduate courses. This book is not a comprehensive view of the field, but a selection of the issues currently at the forefront of knowledge. |
| cell cycle analysis protocol: Cell Cycle Regulation Philipp Kaldis, 2006-06-26 This book is a state-of-the-art summary of the latest achievements in cell cycle control research with an outlook on the effect of these findings on cancer research. The chapters are written by internationally leading experts in the field. They provide an updated view on how the cell cycle is regulated in vivo, and about the involvement of cell cycle regulators in cancer. |
| cell cycle analysis protocol: Microbiology Nina Parker, OpenStax, Mark Schneegurt, AnhHue Thi Tu, Brian M. Forster, Philip Lister, 2016-05-30 Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology's art program enhances students' understanding of concepts through clear and effective illustrations, diagrams, and photographs. Microbiology is produced through a collaborative publishing agreement between OpenStax and the American Society for Microbiology Press. The book aligns with the curriculum guidelines of the American Society for Microbiology.--BC Campus website. |
| cell cycle analysis protocol: Recent Advances in Cytometry , 2011 |
| cell cycle analysis protocol: Flow Cytometry Zbigniew Darzynkiewicz, J. Paul Robinson, 1994 Flow Cytometry, Second Edition is a complete and comprehensive two-volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who use flow cytometry to separate and analyze cells. Key Features * Completely revised and greatly expanded since the publication of the First Edition in 1990 * Unique comprehensive methodological coverage * In-depth treatment of procedures, including: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who developed or modified the techniques * Methods cover cell death and cell cycle analyses * Practical, handbook-style presentation works in lab and in the classroom * Color plates illustrate technique |
| cell cycle analysis protocol: Neuronal Cell Culture Shohreh Amini, Martyn K. White, 2022-06-09 This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies. |
Cell Preparation for Cell Cycle Analysis using Flow Cytometry …
This protocol explains how to prepare cells for cell cycle study using Flow Cytometry. When intracellular staining or fluorescent proteins and/or protein-based fluorochromes are present,
DNA Cell Cycle Analysis with PI - Cornell University College of ...
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isolated nuclei. The PI …
Cell Cycle Analysis - University of Wisconsin Carbone Cancer …
Sep 18, 2017 · The most straightforward method for cell cycle analysis is to fix the cells with ethanol, treat with RNase, and stain with PI. However, different staining protocols may be …
Cell Cycle Analysis by Propidium Iodide Staining
This is a method for cell cycle analysis using propidium iodide (PI), that is, using the fluorescent nucleic acid dye PI to identify the proportion of cells that are in one of the three interphase stages
Propidium iodide staining of cells for cell cycle analysis - Bio …
This method provides a general procedure for DNA staining for cell cycle analysis using propidium iodide (PI). These are guidelines only and the incubation times may need to be adjusted for …
Cell Cycle Analysis using Hoechst 33342 in Unfixed Cells
The optimal Hoechst dye concentration and staining time for different cell types vary as dye up-take depends on cellular metabolic rates; thus, both have to be determined empirically.
Preparing Cells for FACS/PI Analysis (Cell Cycle Analysis)
Preparing cells for PI/FACS (cell cycle) analysis Experimental design considerations - This method works well to assess cell cycle distribution of whole cell populations
Cell Cycle Tutorial - Queen Mary University of London
There are numerous fluorescent DNA dyes used for multiple purposes not all are suitable for flow cytometric cell cycle analysis. Only fluorescent DNA dyes that bind to DNA in a linear manner …
Analysis of Cell Cycle by Flow Cytometry - Trinity College Dublin
These methods can be grouped into three categories: 1. In the first are the methods that rely on a single time point (“snapshot”) cell measurement.
Cell Cycle Determination Using DAPI PFA fixation - University …
Cell Cycle Determination Using DAPI – PFA fixation DAPI is commonly used in cell cycle analyses since preferentially binds dsDNA when cells are permeabilized allowing it to saturate …
Cell Cycle Analysis - LMU
The cell cycle profile of a sample can be determined by staining the DNA with a fluorescent dye and measuring its intensity. From 5 different cell cycle-phases only 3 can be distinguished by …
Cell cycle analyzes using flowcytometry - biomed.au.dk
Several companies sell products for live-cell cell-cycle analysis. Start out with the company’s recommended protocol. The optimal dye concentration must be found for each cell type by …
Cell Cycle Analysis
The most straightforward method for cell cycle analysis is to fix the cells with ethanol, treat with RNase, and stain with PI. However, different staining protocols may be necessary for some …
BD Cell Cycle-DNA Kits and Templates - BD Biosciences
BD cell cycle/DNA kits, protocols, and software templates for the BD AccuriTM C6 flow cytometer simplify the assessment of cell cycle and DNA status.
DAPI staining for cell cycle analysis - LMU
Prepare always an asynchronous culture (untreated) to fix the positions of the G1 and G2 peaks.
Cell Cycle Analysis - Rutgers University
Use nitrogen starved haploid cells, exponentially growing haploids and exponentially growing diploid cells respectively. You can fix large numbers of cells and use them over many months. …
Surface Staining with H33342 Cell Cycle Analysis on Fixed Cells
Primary cultures with stem cell-like properties will efflux H33342 resulting in poor saturation. In this circumstance, fixation and permeabilization is necessary to achieve good DNA content …
Proliferation and Cell Cycle Analysis Using the NovoCyte Flow …
Once a cell becomes committed to undergoing cell division, it progresses through distinct steps of the cell cycle that are regulated by key signaling proteins, which function as gatekeepers. …
Cell-Cycle Analysis Using the BD FACSArray™ Bioanalyzer
There are three distinct, successive stages within interphase, called G1, S, and G2 phases. During G1 (first gap), cells monitor their environment and grow (synthesize RNA and proteins). …
Cell Cycle Determination Using DAPI – Alcohol Fixation Method
The protocol below may be used for DAPI measurements alone and should not be combined with other dyes or surface markers since alcohol based fixatives degrade the cellular membrane as …
Cell Preparation for Cell Cycle Analysis using Flow Cytometry …
This protocol explains how to prepare cells for cell cycle study using Flow Cytometry. When intracellular staining or fluorescent proteins and/or protein-based fluorochromes are present,
DNA Cell Cycle Analysis with PI - Cornell University College …
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isolated nuclei. The PI …
Cell Cycle Analysis - University of Wisconsin Carbone Cancer …
Sep 18, 2017 · The most straightforward method for cell cycle analysis is to fix the cells with ethanol, treat with RNase, and stain with PI. However, different staining protocols may be …
Cell Cycle Analysis by Propidium Iodide Staining
This is a method for cell cycle analysis using propidium iodide (PI), that is, using the fluorescent nucleic acid dye PI to identify the proportion of cells that are in one of the three interphase stages
Propidium iodide staining of cells for cell cycle analysis - Bio …
This method provides a general procedure for DNA staining for cell cycle analysis using propidium iodide (PI). These are guidelines only and the incubation times may need to be adjusted for …
Cell Cycle Analysis using Hoechst 33342 in Unfixed Cells
The optimal Hoechst dye concentration and staining time for different cell types vary as dye up-take depends on cellular metabolic rates; thus, both have to be determined empirically.
Preparing Cells for FACS/PI Analysis (Cell Cycle Analysis)
Preparing cells for PI/FACS (cell cycle) analysis Experimental design considerations - This method works well to assess cell cycle distribution of whole cell populations
Cell Cycle Tutorial - Queen Mary University of London
There are numerous fluorescent DNA dyes used for multiple purposes not all are suitable for flow cytometric cell cycle analysis. Only fluorescent DNA dyes that bind to DNA in a linear manner …
Analysis of Cell Cycle by Flow Cytometry - Trinity College …
These methods can be grouped into three categories: 1. In the first are the methods that rely on a single time point (“snapshot”) cell measurement.
Cell Cycle Determination Using DAPI PFA fixation - University …
Cell Cycle Determination Using DAPI – PFA fixation DAPI is commonly used in cell cycle analyses since preferentially binds dsDNA when cells are permeabilized allowing it to saturate …
Cell Cycle Analysis - LMU
The cell cycle profile of a sample can be determined by staining the DNA with a fluorescent dye and measuring its intensity. From 5 different cell cycle-phases only 3 can be distinguished by …
Cell cycle analyzes using flowcytometry - biomed.au.dk
Several companies sell products for live-cell cell-cycle analysis. Start out with the company’s recommended protocol. The optimal dye concentration must be found for each cell type by …
Cell Cycle Analysis
The most straightforward method for cell cycle analysis is to fix the cells with ethanol, treat with RNase, and stain with PI. However, different staining protocols may be necessary for some …
BD Cell Cycle-DNA Kits and Templates - BD Biosciences
BD cell cycle/DNA kits, protocols, and software templates for the BD AccuriTM C6 flow cytometer simplify the assessment of cell cycle and DNA status.
DAPI staining for cell cycle analysis - LMU
Prepare always an asynchronous culture (untreated) to fix the positions of the G1 and G2 peaks.
Cell Cycle Analysis - Rutgers University
Use nitrogen starved haploid cells, exponentially growing haploids and exponentially growing diploid cells respectively. You can fix large numbers of cells and use them over many months. …
Surface Staining with H33342 Cell Cycle Analysis on Fixed Cells
Primary cultures with stem cell-like properties will efflux H33342 resulting in poor saturation. In this circumstance, fixation and permeabilization is necessary to achieve good DNA content …
Proliferation and Cell Cycle Analysis Using the NovoCyte …
Once a cell becomes committed to undergoing cell division, it progresses through distinct steps of the cell cycle that are regulated by key signaling proteins, which function as gatekeepers. …
Cell-Cycle Analysis Using the BD FACSArray™ Bioanalyzer
There are three distinct, successive stages within interphase, called G1, S, and G2 phases. During G1 (first gap), cells monitor their environment and grow (synthesize RNA and proteins). …
Cell Cycle Determination Using DAPI – Alcohol Fixation …
The protocol below may be used for DAPI measurements alone and should not be combined with other dyes or surface markers since alcohol based fixatives degrade the cellular membrane as …
