| cell culture bioprocess engineering: Cell Culture Bioprocess Engineering, Second Edition Wei-Shou Hu, 2020-03-06 This book is the culmination of three decades of accumulated experience in teaching biotechnology professionals. It distills the fundamental principles and essential knowledge of cell culture processes from across many different disciplines and presents them in a series of easy-to-follow, comprehensive chapters. Practicality, including technological advances and best practices, is emphasized. This second edition consists of major updates to all relevant topics contained within this work. The previous edition has been successfully used in training courses on cell culture bioprocessing over the past seven years. The format of the book is well-suited to fast-paced learning, such as is found in the intensive short course, since the key take-home messages are prominently highlighted in panels. The book is also well-suited to act as a reference guide for experienced industrial practitioners of mammalian cell cultivation for the production of biologics. |
| cell culture bioprocess engineering: Cell Culture Engineering Wei-Shu Hu, 2006-08-16 Since the introduction of recombinant human growth hormone and insulin a quarter century ago, protein therapeutics has greatly broadened the ho- zon of health care. Many patients suffering with life-threatening diseases or chronic dysfunctions, which were medically untreatable not long ago, can attest to the wonder these drugs have achieved. Although the ?rst generation of p- tein therapeutics was produced in recombinant Escherichia coli, most recent products use mammalian cells as production hosts. Not long after the ?rst p- duction of recombinant proteins in E. coli, it was realized that the complex tasks of most post-translational modi?cations on proteins could only be ef?ciently carried out in mammalian cells. In the 1990s, we witnessed a rapid expansion of mammalian-cell-derived protein therapeutics, chie?y antibodies. In fact, it has been nearly a decade since the market value of mammalian-cell-derived protein therapeutics surpassed that of those produced from E. coli. A common characteristic of recent antibody products is the relatively large dose required for effective therapy, demanding larger quantities for the treatment of a given disease. This, coupled with the broadening repertoire of protein drugs, has rapidly expanded the quantity needed for clinical applications. The increasing demand for protein therapeutics has not been met exclusively by construction of new manufacturing plants and increasing total volume capacity. More - portantly the productivity of cell culture processes has been driven upward by an order of magnitude in the past decade. |
| cell culture bioprocess engineering: Bioreactors Goutam Saha, Alok Barua, Satyabroto Sinha, 2017-12-01 Bioreactors: Animal Cell Culture Control for Bioprocess Engineering presents the design, fabrication, and control of a new type of bioreactor meant especially for animal cell line culture. The new bioreactor, called the see-saw bioreactor, is ideal for the growth of cells with a sensitive membrane. The see-saw bioreactor derives its name from its principle of operation in which liquid columns in either limb of the reactor alternately go up and down. The working volume of the reactor is small, to within 15 L. However, it can easily be scaled up for large production in volume of cell mass in the drug and pharmaceutical industries. The authors describe the principle of operation of the see-saw bioreactor and how to automatically control the bioprocess. They discuss different control strategies as well as the thorough experimental research they conducted on this prototype bioreactor in which they applied a time delay control for yield maximization. To give you a complete understanding of the design and development of the see-saw bioreactor, the authors cover the mathematical model they use to describe the kinetics of fermentation, the genetic algorithms used for deriving the optimal time trajectories of the bioprocess variables, and the corresponding control inputs for maximizing the product yield. One chapter is devoted to the application of time delay control. Following a description of the bioreactor’s working setup in the laboratory, the authors sum up their investigation and define the future scope of work in terms of design, control, and software sensors. |
| cell culture bioprocess engineering: Cell Culture Engineering Gyun Min Lee, Helene Faustrup Kildegaard, 2020-01-13 Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned Advanced Biotechnology book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences. |
| cell culture bioprocess engineering: Insect Cell Culture Engineering Mattheus F. A. Goosen, 2020-07-24 Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines. |
| cell culture bioprocess engineering: Bioprocess Engineering Shijie Liu, 2012-11-21 Bioprocess Engineering involves the design and development of equipment and processes for the manufacturing of products such as food, feed, pharmaceuticals, nutraceuticals, chemicals, and polymers and paper from biological materials. It also deals with studying various biotechnological processes. Bioprocess Kinetics and Systems Engineering first of its kind contains systematic and comprehensive content on bioprocess kinetics, bioprocess systems, sustainability and reaction engineering. Dr. Shijie Liu reviews the relevant fundamentals of chemical kinetics-including batch and continuous reactors, biochemistry, microbiology, molecular biology, reaction engineering, and bioprocess systems engineering- introducing key principles that enable bioprocess engineers to engage in the analysis, optimization, design and consistent control over biological and chemical transformations. The quantitative treatment of bioprocesses is the central theme of this book, while more advanced techniques and applications are covered with some depth. Many theoretical derivations and simplifications are used to demonstrate how empirical kinetic models are applicable to complicated bioprocess systems. - Contains extensive illustrative drawings which make the understanding of the subject easy - Contains worked examples of the various process parameters, their significance and their specific practical use - Provides the theory of bioprocess kinetics from simple concepts to complex metabolic pathways - Incorporates sustainability concepts into the various bioprocesses |
| cell culture bioprocess engineering: Bioprocess Engineering Michael L. Shuler, Fikret Kargi, 2014 For Senior-level and graduate courses in Biochemical Engineering, and for programs in Agricultural and Biological Engineering or Bioengineering. This concise yet comprehensive text introduces the essential concepts of bioprocessing-internal structure and functions of different types of microorganisms, major metabolic pathways, enzymes, microbial genetics, kinetics and stoichiometry of growth and product information-to traditional chemical engineers and those in related disciplines. It explores the engineering principles necessary for bioprocess synthesis and design, and illustrates the application of these principles to modern biotechnology for production of pharmaceuticals and biologics, solution of environmental problems, production of commodities, and medical applications. |
| cell culture bioprocess engineering: Bioprocess Engineering Kim Gail Clarke, 2013-10-31 Biotechnology is an expansive field incorporating expertise in both the life science and engineering disciplines. In biotechnology, the scientist is concerned with developing the most favourable biocatalysts, while the engineer is directed towards process performance, defining conditions and strategies that will maximize the production potential of the biocatalyst. Increasingly, the synergistic effect of the contributions of engineering and life sciences is recognised as key to the translation of new bioproducts from the laboratory bench to commercial bioprocess. Fundamental to the successful realization of the bioprocess is a need for process engineers and life scientists competent in evaluating biological systems from a cross-disciplinary viewpoint. Bioprocess engineering aims to generate core competencies through an understanding of the complementary biotechnology disciplines and their interdependence, and an appreciation of the challenges associated with the application of engineering principles in a life science context. Initial chapters focus on the microbiology, biochemistry and molecular biology that underpin biocatalyst potential for product accumulation. The following chapters develop kinetic and mass transfer principles that quantify optimum process performance and scale up. The text is wide in scope, relating to bioprocesses using bacterial, fungal and enzymic biocatalysts, batch, fed-batch and continuous strategies and free and immobilised configurations. - Details the application of chemical engineering principles for the development, design, operation and scale up of bioprocesses - Details the knowledge in microbiology, biochemistry and molecular biology relevant to bioprocess design, operation and scale up - Discusses the significance of these life sciences in defining optimum bioprocess performance |
| cell culture bioprocess engineering: Biochemical Engineering and Biotechnology Ghasem Najafpour, 2015-02-24 Biochemical Engineering and Biotechnology, 2nd Edition, outlines the principles of biochemical processes and explains their use in the manufacturing of every day products. The author uses a diirect approach that should be very useful for students in following the concepts and practical applications. This book is unique in having many solved problems, case studies, examples and demonstrations of detailed experiments, with simple design equations and required calculations. - Covers major concepts of biochemical engineering and biotechnology, including applications in bioprocesses, fermentation technologies, enzymatic processes, and membrane separations, amongst others - Accessible to chemical engineering students who need to both learn, and apply, biological knowledge in engineering principals - Includes solved problems, examples, and demonstrations of detailed experiments with simple design equations and all required calculations - Offers many graphs that present actual experimental data, figures, and tables, along with explanations |
| cell culture bioprocess engineering: Bioprocess Engineering Principles Pauline M. Doran, 1995-04-03 The emergence and refinement of techniques in molecular biology has changed our perceptions of medicine, agriculture and environmental management. Scientific breakthroughs in gene expression, protein engineering and cell fusion are being translated by a strengthening biotechnology industry into revolutionary new products and services. Many a student has been enticed by the promise of biotechnology and the excitement of being near the cutting edge of scientific advancement. However, graduates trained in molecular biology and cell manipulation soon realise that these techniques are only part of the picture. Reaping the full benefits of biotechnology requires manufacturing capability involving the large-scale processing of biological material. Increasingly, biotechnologists are being employed by companies to work in co-operation with chemical engineers to achieve pragmatic commercial goals. For many years aspects of biochemistry and molecular genetics have been included in chemical engineering curricula, yet there has been little attempt until recently to teach aspects of engineering applicable to process design to biotechnologists.This textbook is the first to present the principles of bioprocess engineering in a way that is accessible to biological scientists. Other texts on bioprocess engineering currently available assume that the reader already has engineering training. On the other hand, chemical engineering textbooks do not consider examples from bioprocessing, and are written almost exclusively with the petroleum and chemical industries in mind. This publication explains process analysis from an engineering point of view, but refers exclusively to the treatment of biological systems. Over 170 problems and worked examples encompass a wide range of applications, including recombinant cells, plant and animal cell cultures, immobilised catalysts as well as traditional fermentation systems.* * First book to present the principles of bioprocess engineering in a way that is accessible to biological scientists* Explains process analysis from an engineering point of view, but uses worked examples relating to biological systems* Comprehensive, single-authored* 170 problems and worked examples encompass a wide range of applications, involving recombinant plant and animal cell cultures, immobilized catalysts, and traditional fermentation systems* 13 chapters, organized according to engineering sub-disciplines, are groupled in four sections - Introduction, Material and Energy Balances, Physical Processes, and Reactions and Reactors* Each chapter includes a set of problems and exercises for the student, key references, and a list of suggestions for further reading* Includes useful appendices, detailing conversion factors, physical and chemical property data, steam tables, mathematical rules, and a list of symbols used* Suitable for course adoption - follows closely curricula used on most bioprocessing and process biotechnology courses at senior undergraduate and graduate levels. |
| cell culture bioprocess engineering: Mammalian Cell Cultures for Biologics Manufacturing Weichang Zhou, Anne Kantardjieff, 2014-01-15 Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English. |
| cell culture bioprocess engineering: Cell Culture Technology for Pharmaceutical and Cell-Based Therapies Sadettin Ozturk, Wei-Shou Hu, 2005-08-30 Edited by two of the most distinguished pioneers in genetic manipulation and bioprocess technology, this bestselling reference presents a comprehensive overview of current cell culture technology used in the pharmaceutical industry. Contributions from several leading researchers showcase the importance of gene discovery and genomic technology devel |
| cell culture bioprocess engineering: Advances in Bioprocess Engineering Enrique Galindo, Octavio R. Ramírez, 2013-04-17 Bioprocess engineering has played a key role in biotechnology, contributing towards bringing the exciting new discoveries of molecular and cellular biology into the applied sphere, and in maintaining established processes, some centuries-old, efficient and essential for today's industry. Novel developments and new application areas of biotechnology, along with increasing constraints in costs, product quality, regulatory and environmental considerations, have placed the biochemical engineer at the forefront of new challenges. This second volume of Advances in Bioprocess Engineering reflects precisely the multidisciplinary nature of the field, where new and traditional areas of application are nurtured by a better understanding of fundamental phenomena and by the utilization of novel techniques and methodologies. The chapters in this book were written by the invited speakers to the 2nd International Symposium on Bioprocess Engineering, Mazatlan, Mexico, September 1997. |
| cell culture bioprocess engineering: Putting Biotechnology to Work National Research Council, Division on Earth and Life Studies, Commission on Life Sciences, Committee on Bioprocess Engineering, 1992-02-01 The ability of the United States to sustain a dominant global position in biotechnology lies in maintaining its primacy in basic life-science research and developing a strong resource base for bioprocess engineering and bioproduct manufacturing. This book examines the status of bioprocessing and biotechnology in the United States; current bioprocess technology, products, and opportunities; and challenges of the future and what must be done to meet those challenges. It gives recommendations for action to provide suitable incentives to establish a national program in bioprocess-engineering research, development, education, and technology transfer. |
| cell culture bioprocess engineering: Fundamentals of Modern Bioprocessing Sarfaraz K. Niazi, Justin L. Brown, 2017-07-27 Biological drug and vaccine manufacturing has quickly become one of the highest-value fields of bioprocess engineering, and many bioprocess engineers are now finding job opportunities that have traditionally gone to chemical engineers. Fundamentals of Modern Bioprocessing addresses this growing demand. Written by experts well-established in the field, this book connects the principles and applications of bioprocessing engineering to healthcare product manufacturing and expands on areas of opportunity for qualified bioprocess engineers and students. The book is divided into two sections: the first half centers on the engineering fundamentals of bioprocessing; while the second half serves as a handbook offering advice and practical applications. Focused on the fundamental principles at the core of this discipline, this work outlines every facet of design, component selection, and regulatory concerns. It discusses the purpose of bioprocessing (to produce products suitable for human use), describes the manufacturing technologies related to bioprocessing, and explores the rapid expansion of bioprocess engineering applications relevant to health care product manufacturing. It also considers the future of bioprocessing—the use of disposable components (which is the fastest growing area in the field of bioprocessing) to replace traditional stainless steel. In addition, this text: Discusses the many types of genetically modified organisms Outlines laboratory techniques Includes the most recent developments Serves as a reference and contains an extensive bibliography Emphasizes biological manufacturing using recombinant processing, which begins with creating a genetically modified organism using recombinant techniques Fundamentals of Modern Bioprocessing outlines both the principles and applications of bioprocessing engineering related to healthcare product manufacturing. It lays out the basic concepts, definitions, methods and applications of bioprocessing. A single volume comprehensive reference developed to meet the needs of students with a bioprocessing background; it can also be used as a source for professionals in the field. |
| cell culture bioprocess engineering: Fermentation and Biochemical Engineering Handbook Henry C. Vogel, Celeste M. Todaro, 1996-12-31 This is a well-rounded handbook of fermentation and biochemical engineering presenting techniques for the commercial production of chemicals and pharmaceuticals via fermentation. Emphasis is given to unit operations fermentation, separation, purification, and recovery. Principles, process design, and equipment are detailed. Environment aspects are covered. The practical aspects of development, design, and operation are stressed. Theory is included to provide the necessary insight for a particular operation. Problems addressed are the collection of pilot data, choice of scale-up parameters, selection of the right piece of equipment, pinpointing of likely trouble spots, and methods of troubleshooting. The text, written from a practical and operating viewpoint, will assist development, design, engineering and production personnel in the fermentation industry. Contributors were selected based on their industrial background and orientation. The book is illustrated with numerous figures, photographs and schematic diagrams. |
| cell culture bioprocess engineering: Bioprocessing Owen P. Ward, 2012-12-06 Methods for processing of biological materials into useful products represent essential core manufacturing activities of the food, chemical and pharmaceutical industries. On the one hand the techniques involved include well established process engineering methodologies such as mixing, heat transfer, size modification and a variety of separatIon and fermentation procedures. In addition, new bioprocessing practices arising from the exciting recent advances in biotech nology, including innovative fermentation cell culture and enzyme based operations, are rapidly extending the frontiers of bioprocessing. These develop ments are resulting in the introduction to the market place of an awesome range of novel biological products having unique applications. Indeed, the United States Office of Technology Assessment· has concluded that 'competitive advantage in areas related to biotechnology may depend as much on developments in bioprocess engineering as on innovations in genetics, immunology and other areas of basic science'. Advances in analytical instrumentation, computerization and process automation are playing an important role in process control and optimization and in the maintenance of product quality and consistency characteristics. Bioprocessing represents the industrial practice of biotechnology and is multidisciplinary in nature, integrating the biological, chemical and engineering sciences. This book discusses the individual unit operations involved and describes a wide variety of important industrial bioprocesses. I am very grateful to Sanjay Thakur who assisted me in the collection of material for this book. |
| cell culture bioprocess engineering: Cell and Tissue Reaction Engineering Regine Eibl, Dieter Eibl, Ralf Pörtner, Gerardo Catapano, Peter Czermak, 2008-09-30 The completion of the Human Genome Project and the rapid progress in cell bi- ogy and biochemical engineering, are major forces driving the steady increase of approved biotech products, especially biopharmaceuticals, in the market. Today mammalian cell products (“products from cells”), primarily monoclonals, cytokines, recombinant glycoproteins, and, increasingly, vaccines, dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells (“cells as product”) for regenerative medicine have also been introduced in the market. Their efficient production requires comprehensive knowledge of biological as well as biochemical mammalian cell culture fundamentals (e.g., cell characteristics and metabolism, cell line establishment, culture medium optimization) and related engineering principles (e.g., bioreactor design, process scale-up and optimization). In addition, new developments focusing on cell line development, animal-free c- ture media, disposables and the implications of changing processes (multi-purpo- facilities) have to be taken into account. While a number of excellent books treating the basic methods and applications of mammalian cell culture technology have been published, only little attention has been afforded to their engineering aspects. The aim of this book is to make a contribution to closing this gap; it particularly focuses on the interactions between biological and biochemical and engineering principles in processes derived from cell cultures. It is not intended to give a c- prehensive overview of the literature. This has been done extensively elsewhere. |
| cell culture bioprocess engineering: Bioprocess Engineering Bjorn K. Lydersen, Nancy A. D'Elia, Kim L. Nelson, 1994-04-18 Divided into four sections, the first and third reflect the fact that there are two types of equipment required in the plant--one in which the actual product is synthesized or processed such as the fermentor, centrifuge and chromatographic columns; and the other that supplies support for the facility or process including air conditioning, water and waste systems. Part two describes such components as pumps, filters and valves not limited to a certain type of equipment. Lastly, it covers planning and designing the entire facility along with requirements for containment and validation of the process. |
| cell culture bioprocess engineering: Cell Culture Bioprocess Engineering, Second Edition Wei-Shou Hu, 2020-03-06 This book is the culmination of three decades of accumulated experience in teaching biotechnology professionals. It distills the fundamental principles and essential knowledge of cell culture processes from across many different disciplines and presents them in a series of easy-to-follow, comprehensive chapters. Practicality, including technological advances and best practices, is emphasized. This second edition consists of major updates to all relevant topics contained within this work. The previous edition has been successfully used in training courses on cell culture bioprocessing over the past seven years. The format of the book is well-suited to fast-paced learning, such as is found in the intensive short course, since the key take-home messages are prominently highlighted in panels. The book is also well-suited to act as a reference guide for experienced industrial practitioners of mammalian cell cultivation for the production of biologics. |
| cell culture bioprocess engineering: Industrial Biotechnology Christoph Wittmann, James C. Liao, 2017-03-06 The latest volume in the Advanced Biotechnology series provides an overview of the main product classes and platform chemicals produced by biotechnological processes today, with applications in the food, healthcare and fine chemical industries. Alongside the production of drugs and flavors as well as amino acids, bio-based monomers and polymers and biofuels, basic insights are also given as to the biotechnological processes yielding such products and how large-scale production may be enabled and improved. Of interest to biotechnologists, bio and chemical engineers, as well as those working in the biotechnological, chemical, and food industries. |
| cell culture bioprocess engineering: Engineering Principles in Biotechnology Wei-Shou Hu, 2017-11-13 This book is a short introduction to the engineering principles of harnessing the vast potential of microorganisms, and animal and plant cells in making biochemical products. It was written for scientists who have no background in engineering, and for engineers with minimal background in biology. The overall subject dealt with is process. But the coverage goes beyond the process of biomanufacturing in the bioreactor, and extends to the factory of cell’s biosynthetic machinery. Starting with an overview of biotechnology and organism, engineers are eased into biochemical reactions and life scientists are exposed to the technology of production using cells. Subsequent chapters allow engineers to be acquainted with biochemical pathways, while life scientist learn about stoichiometric and kinetic principles of reactions and cell growth. This leads to the coverage of reactors, oxygen transfer and scale up. Following three chapters on biomanufacturing of current and future importance, i.e. cell culture, stem cells and synthetic biology, the topic switches to product purification, first with a conceptual coverage of operations used in bioseparation, and then a more detailed analysis to provide a conceptual understanding of chromatography, the modern workhorse of bioseparation. Drawing on principles from engineering and life sciences, this book is for practitioners in biotechnology and bioengineering. The author has used the book for a course for advanced students in both engineering and life sciences. To this end, problems are provided at the end of each chapter. |
| cell culture bioprocess engineering: Animal Cell Bioreactors Chester S. Ho, 2013-10-22 Animal Cell Bioreactors provides an introduction to the underlying principles and strategies in the in vitro cell culture biotechnology. It addresses engineering aspects such as mass transfer, instrumentation, and control ensuring successful design and operation of animal cell bioreactors. The goal is to provide a comprehensive analysis and review in the advancement of the bioreactor systems for large-scale animal cell cultures. The book is organized into four parts. Part I traces the historical development of animal cell biotechnology. It presents examples of work in progress that seeks to make animal cell biotechnology processes as productive on a cost per unit of product basis as that achieved by other microbial systems. Part II includes chapters dealing with the implications of cell biology in animal cell biotechnology; protein-bound oligosaccharides and their structures; the development of serum-free media and its use in the production of biologically active substances; and the metabolism of mammalian cells. Part III focuses on animal cell cultivation, covering topics such as the fixed bed immobilized culture; three-dimensional microcarriers; and hydrodynamic phenomena in microcarrier cultures. Part IV discusses the design, operation, and control of animal cell bioreactors. |
| cell culture bioprocess engineering: An Introduction To Metabolic And Cellular Engineering Miguel Antonio Aon, Sonia Del Carmen Cortassa, Alberto Alvaro Iglesias, David Lloyd, 2002-03-07 Metabolic and cellular engineering, as presented in this book, is a powerful alliance of two technologies: genetics-molecular biology and fermentation technology. Both are driven by continuous refinement of the basic understanding of metabolism, physiology and cellular biology (growth, division, differentiation), as well as the development of new mathematical modeling techniques. The authors' approach is original in that it integrates several disciplines into a coordinated scheme, i.e. microbial physiology and bioenergetics, thermodynamics and enzyme kinetics, biomathematics and biochemistry, genetics and molecular biology. Thus, it is called a transdisciplinary approach (TDA). The TDA provides the basis for the rational design of microorganisms or cells in a way that has rarely been utilized to its full extent. |
| cell culture bioprocess engineering: Cell Line Development Mohamed Al-Rubeai, 2009-08-11 Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms. This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout. |
| cell culture bioprocess engineering: Stem Cell Bioprocessing Tiago G. Fernandes, M. Margardia Diogo, Joaquim M.S. Cabral, 2013-11-15 Stem cell bioprocessing describes the main large-scale bioprocessing strategies for both stem cell culture and purification, envisaging the application of these cells for regenerative medicine and drug screening. Bioreactor configurations are described, including their applications for stem cell expansion, and stem cell separation techniques such as isolation and purification are discussed. Basic definitions are provided concerning the different types of stem cells, from adult stem cells to the more recent induced pluripotent stem cells. The main characteristics of these different stem cell types are described, alongside the molecular mechanisms underlying their self-renewal and differentiation. The book also focuses on methodologies currently used for in vitro stem cell culture under static conditions, including the challenge of xeno-free culture conditions, as well as culture parameters that influence stem cell culture. Approaches for both stem cell culture and separation in micro-scale conditions are presented, including the use of cellular microarrays for high-throughput screening of the effect of both soluble and extracellular matrix molecules. A further section is dedicated to application of stem cells for regenerative medicine. - Maintains a unique focus on both the basic stem cell biology concepts, and their translation to large-scale bioprocessing approaches - Envisages the use of stem cells in regenerative medicine and drug screening applications - Discusses the application of microscale techniques as a tool to perform basic stem cell biology studies |
| cell culture bioprocess engineering: Integrated Bioprocess Engineering Clemens Posten, 2024-07-01 Bioprocess engineering employs microorganisms to produce biological products for medical and industrial applications. The book covers engineering tasks around- the cultivation process in bioreactors including media design, feeding strategies, or cell harvesting. All aspects are described from the conceptual considerations to technical realization and provide insight by detailed explanations, drawings, formulas, and example processes. |
| cell culture bioprocess engineering: Manual of Industrial Microbiology and Biotechnology Arnold L. Demain, Julian E. Davies, Ronald M. Atlas, 1999 The editors have enlisted a broad range of experts, including microbial ecologists, physiologists, geneticists, biochemists, molecular biologists, and biochemical engineers, who offer practical experience not found in texts and journals. This comprehensive perspective makes MIMB a valuable how to resource, the structure of which resembles the sequence of operation involved in the development of a commercial biological process and product. |
| cell culture bioprocess engineering: Cell Culture Technology Cornelia Kasper, Verena Charwat, Antonina Lavrentieva, 2018-10-10 This textbook provides an overview on current cell culture techniques, conditions, and applications specifically focusing on human cell culture. This book is based on lectures, seminars and practical courses in stem cells, tissue engineering, regenerative medicine and 3D cell culture held at the University of Natural Resources and Life Sciences Vienna BOKU and the Gottfried Wilhelm Leibniz University Hannover, complemented by contributions from international experts, and therefore delivers in a compact and clear way important theoretical, as well as practical knowledge to advanced graduate students on cell culture techniques and the current status of research. The book is written for Master students and PhD candidates in biotechnology, tissue engineering and biomedicine working with mammalian, and specifically human cells. It will be of interest to doctoral colleges, Master- and PhD programs teaching courses in this area of research. |
| cell culture bioprocess engineering: Cell Culture and Upstream Processing Michael Butler, 2007-08-07 Each chapter in Cell Culture and Upstream Processing is taken from a presentation at the highly acclaimed IBC conferences and describe ways for scientists to improve yield and optimize the cell culture production process for biopharmaceuticals, by focusing on safety, quality, economics and operability and productivity issues. |
| cell culture bioprocess engineering: Bioprocesses Including Animal Cell Culture A. Fiechter, 1988 |
| cell culture bioprocess engineering: Cell Culture Engineering and Technology Ralf Pörtner, 2022-02-20 This contributed volume is dedicated towards the progress achieved within the last years in all areas of Cell Culture Engineering and Technology. It comprises contributions of active researchers in the field of cell culture development for the production of recombinant proteins, cell line development, cell therapy and gene therapy, with consideration of media development, process scale-up, reactor design, monitoring and control and model-assisted strategies for process design. The knowledge and expertise of the authors cover disciplines like cell biology, engineering, biotechnology and biomedical sciences. This book is conceived for graduate students, postdoctoral fellows and researchers interested in the latest developments in Cell Engineering. |
| cell culture bioprocess engineering: Bioprocess Engineering Principles Ross Carlson, Kate Morrissey, 2024-09-27 Bioprocess Engineering Principles, Third Edition provides a solid introduction to bioprocess engineering for students with a limited engineering background. The book explains process analysis from an engineering perspective using worked examples and problems that relate to biological systems. Application of engineering concepts is illustrated in areas of modern biotechnology, such as recombinant protein production, bioremediation, biofuels, drug development, and tissue engineering, as well as microbial fermentation. With new and expanded material, this remains the book of choice for students seeking to move into bioprocess engineering - Includes more than 350 problems that demonstrate how fundamental principles are applied in areas such as biofuels, bioplastics, bioremediation, tissue engineering, site-directed mutagenesis, recombinant protein production, and drug development, as well as for traditional microbial fermentation - Provides in-depth treatment of fluid flow, turbulence, mixing, and impeller design, reflecting recent advances in our understanding of mixing processes and their importance in determining the performance of cell cultures - Focuses on underlying scientific and engineering principles rather than on specific biotechnology applications, providing a sound basis for teaching bioprocess engineering - Presents new or expanded coverage of such topics as enzyme kinetics, downstream processing, disposable reactors, genetic engineering, and the technology of fermentation |
| cell culture bioprocess engineering: Cell and Tissue Reaction Engineering Regine Eibl, Dieter Eibl, Ralf Pörtner, Gerardo Catapano, Peter Czermak, 2008-09-26 The completion of the Human Genome Project and the rapid progress in cell bi- ogy and biochemical engineering, are major forces driving the steady increase of approved biotech products, especially biopharmaceuticals, in the market. Today mammalian cell products (“products from cells”), primarily monoclonals, cytokines, recombinant glycoproteins, and, increasingly, vaccines, dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells (“cells as product”) for regenerative medicine have also been introduced in the market. Their efficient production requires comprehensive knowledge of biological as well as biochemical mammalian cell culture fundamentals (e.g., cell characteristics and metabolism, cell line establishment, culture medium optimization) and related engineering principles (e.g., bioreactor design, process scale-up and optimization). In addition, new developments focusing on cell line development, animal-free c- ture media, disposables and the implications of changing processes (multi-purpo- facilities) have to be taken into account. While a number of excellent books treating the basic methods and applications of mammalian cell culture technology have been published, only little attention has been afforded to their engineering aspects. The aim of this book is to make a contribution to closing this gap; it particularly focuses on the interactions between biological and biochemical and engineering principles in processes derived from cell cultures. It is not intended to give a c- prehensive overview of the literature. This has been done extensively elsewhere. |
| cell culture bioprocess engineering: Bioprocess Engineering Michael L. Shuler, Fikret Kargı, 1992 Textbook for junior and senior level majors in chemical engineering covering the field of biochemical engineering. |
| cell culture bioprocess engineering: Plant Cells J.-J. Zhong, 2003-07-01 Plants produce more than 30,000 types of chemicals, including pharmaceuticals, pigments and other fine chemicals, which is four times more than those obtain ed from microbes. Plant cell culture has been receiving great attention as an alternative for the production of valuable plant derived secondary metabolites, since it has many advantages over whole plant cultivation. However, much more research is required to enhance the culture productivity and reduce the pro cessing costs, which is the key to the commercialization of plant cell culture pro cesses. The recent achievements in related biochemical engineering studies are reviewed in Chapter 1. The effect of gaseous compounds on plant cell behavior has been little studied, and Chapter 2 focuses on these gas concentration effects (including oxygen, carbon dioxide, ethylene and others, such as volatile hor mones like methyl jasmonate) on secondary metabolite production by plant cell cultures. Two metabolites of current interest, i. e. , the antimalarial artemisinin (known as qing hao su in China) that is produced by Artemisia annua (sweet wormwood) and taxanes used for anticancer therapy that are produced by species of Taxus, are taken as examples. Bioprocess integration is another hot topic in plant cell culture technology. Because most of the plant secondary meta bolites are toxic to the cells at high concentrations during the culture, removal of the product in situ during the culture can lead to the enhanced productivity. Various integrated bioprocessing techniques are discussed in Chapter 3. |
| cell culture bioprocess engineering: Advances in Plant Biotechnology D.D.Y. Ryu, Shintaro Furusaki, 2012-12-02 This volume, contributed to by a group of 46 research scientists and engineers, focuses on the integration of two aspects of plant biotechnology - the basic plant science and applied bioprocess engineering. Included in this book are 17 chapters, each dealing with specific topics of current interest with three coherent themes of: plant gene expression, regulation and manipulation; plant cell physiology and metabolism and their regulation; and bioprocess engineering and bioreactor performance of plant cell cultures. All of these topics are integrated into a main theme of enabling plant biotechnology relevant to the production of secondary metabolites. This book will be of great value to all plant cell biologists and molecular geneticists, and all those interested in the integration of plant science and bioprocess engineering for development of enabling technology relevant to the production of plant secondary metabolites. |
| cell culture bioprocess engineering: Bioprocess Engineering Michael L. Shuler, Fikret Kargi, 2002 This concise yet comprehensive text introduces the essential concepts of bioprocessing - internal structure and functions of different types of microorganisms, major metabolic pathways, enzymes, microbial genetics, kinetics and stoichiometry of growth and product information - to traditional chemical engineers and those in related disciplines. It explores the engineering principles necessary for bioprocess synthesis and design, and illustrates the application of these principles to modern biotechnology for production of pharmaceuticals and biologics, solution of environmental problems, production of commodities, and medical applications. |
| cell culture bioprocess engineering: Biosimilars of Monoclonal Antibodies Cheng Liu, K. John Morrow, Jr., 2016-12-09 Addressing a significant need by describing the science and process involved to develop biosimilars of monoclonal antibody (mAb) drugs, this book covers all aspects of biosimilar development: preclinical, clinical, regulatory, manufacturing. • Guides readers through the complex landscape involved with developing biosimilar versions of monoclonal antibody (mAb) drugs • Features flow charts, tables, and figures that clearly illustrate processes and makes the book comprehensible and accessible • Includes a review of FDA-approved mAb drugs as a quick reference to facts and useful information • Examines new technologies and strategies for improving biosimilar mAbs |
| cell culture bioprocess engineering: Plant Tissue Culture Engineering S. Dutta Gupta, Yasuomi Ibaraki, 2006-07-10 It is my privilege to contribute the foreword for this unique volume entitled: “Plant Tissue Culture Engineering,” edited by S. Dutta Gupta and Y. Ibaraki. While there have been a number of volumes published regarding the basic methods and applications of plant tissue and cell culture technologies, and even considerable attention provided to bioreactor design, relatively little attention has been afforded to the engineering principles that have emerged as critical contributions to the commercial applications of plant biotechnologies. This volume, “Plant Tissue Culture Engineering,” signals a turning point: the recognition that this specialized field of plant science must be integrated with engineering principles in order to develop efficient, cost effective, and large scale applications of these technologies. I am most impressed with the organization of this volume, and the extensive list of chapters contributed by expert authors from around the world who are leading the emergence of this interdisciplinary enterprise. The editors are to be commended for their skilful crafting of this important volume. The first two parts provide the basic information that is relevant to the field as a whole, the following two parts elaborate on these principles, and the last part elaborates on specific technologies or applications. |
Cell Culture Bioprocess Engineering - api.pageplace.de
Since this book is written for read-ers of diverse backgrounds, the aim is to help engineers understand the key biological features of cells without requiring an extensive prior back-ground …
Measurement and control of foam generation in a …
Foam is generated in mammalian cell cultures by excessive agitation or gas sparging. This occurs particularly in cultures that generate recombinant proteins at high cell con- centrations.
Ralf Pörtner Editor Cell Culture Engineering and Technology
Aspects of cell culture process engineering, advanced bioreactor concepts, modelling, as well as monitoring and control are addressed by chapters on scale-up (and scale-down) techniques, …
Cell Culture Bioprocess Technology : Biologics and Beyond
In this chapter, you will learn how cell culture progressed from a scientist’s tool to a major technological medium for manufacturing. You will also learn how a new generation of cell …
UNIT I Bioprocess Engineering -1 SBTA1502 - Sathyabama …
SBTA1502/BIOPROCESS ENGINEERING/UNIT I 2019-23 SEM V 4 | P a g e FIGURE 1.1 Bacterial Fermentation Products of Pyruvate THE RANGE OF FERMENTATION …
Lecture Notes in Bioengineering
plant and mammalian cell culture techniques. Nano biotechnology, as a promising technology is also progressing, classically for cancer targeted drug development.
Bioprocess Engineering - INFLIBNET Centre
Bioprocess engineering encompasses the design, operation, control, and optimization of biochemical processes involving various biological pathways or reactions mediated by living …
Cell Culture Bioprocess Engineering - cms.sunhealth.org
Jan 7, 2025 · Bioprocess Engineering presents the design, fabrication, and control of a new type of bioreactor meant especially for animal cell line culture. The new bioreactor, called the see …
Cell Culture Bioprocess Engineering (Download Only)
synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production In addition the book discusses the most …
CHEE 512. STEM CELL BIOPROCESS ENGINEERING.
Introduction to stem cell biology. Cell growth models applicable to stem cells and their progeny. Upstream processing (cell culture systems, bioreactors), downstream processing (cell …
Bioprocessing Basics - Eppendorf
Broadly speaking, bioreactors and fermenters are culture systems to produce cells or organisms. They are used in various applications, including basic research and development, and the …
Chapter 5 Bioreactor Design and Scale-Up - Springer
Design and selection of cell culture bioreactors are affected by cell-specific demands, engineering aspects, as well as economic and regulatory considerations. Mainly special demands, such as …
Unlocking the potential of cultivated meat through cell line …
Cell line engineering can be used to confer a diverse array of phenotypes in mammalian cells relevant for industrial-scale cultivated meat bioprocesses. These phenotypes can not only …
Cell Culture Bioprocess Engineering - openedconsortium.org
Bioprocess Engineering presents the design, fabrication, and control of a new type of bioreactor meant especially for animal cell line culture. The new bioreactor, called the see-saw …
BIOREACTORS AND CULTIVATION SYSTEMS FOR CELL AND …
Regenerative medicine using human primary and stem cells opens new therapeutic concepts. Additionally, plant cell and tissue cultures for the production of biologically active substances …
Cell Culture: An Invaluable Technique for Biomedical Research
Cell culture is an indispensable technique in biomedical research, enabling scientists to explore the intricacies of cell biology, investigate disease mechanisms, and develop innovative therapies.
Bioprocess Engineering: Basic Concepts - Pearson Deutschland
1 WHAT IS A BIOPROCESS ENGINEER? 1.1. Biotechnology and Bioprocess Engineering. 1.2. Differing Approaches to Research for Biologists and Engineers. 1.3. The Story of Penicillin: …
Cell Culture Bioprocess Engineering (2024)
Cell Culture Bioprocess Engineering Wei-Shou Hu,2020 This reference guide is designed for industrial practitioners of mammalian cell cultivation for the production of biologics. This work …
Cell Culture Bioprocess Engineering (book) - old.icapgen.org
principles and essential knowledge of cell culture processes from across many different disciplines and presents them in a series of easy to follow comprehensive chapters Practicality …
Bio Process Engineering Principles - vols.wta.org
It distills the fundamental principles and essential knowledge of cell culture processes from across many different disciplines and presents them in a series of easy-to-follow, comprehensive …
Cell Culture Bioprocess Engineering - api.pageplace.de
Since this book is written for read-ers of diverse backgrounds, the aim is to help engineers understand the key biological features of cells without requiring an extensive prior back-ground …
Measurement and control of foam generation in a mammalian …
Foam is generated in mammalian cell cultures by excessive agitation or gas sparging. This occurs particularly in cultures that generate recombinant proteins at high cell con- centrations.
Ralf Pörtner Editor Cell Culture Engineering and Technology
Aspects of cell culture process engineering, advanced bioreactor concepts, modelling, as well as monitoring and control are addressed by chapters on scale-up (and scale-down) techniques, use …
Cell Culture Bioprocess Technology : Biologics and Beyond
In this chapter, you will learn how cell culture progressed from a scientist’s tool to a major technological medium for manufacturing. You will also learn how a new generation of cell-based …
UNIT I Bioprocess Engineering -1 SBTA1502 - Sathyabama …
SBTA1502/BIOPROCESS ENGINEERING/UNIT I 2019-23 SEM V 4 | P a g e FIGURE 1.1 Bacterial Fermentation Products of Pyruvate THE RANGE OF FERMENTATION PROCESSES There are five …
Lecture Notes in Bioengineering
plant and mammalian cell culture techniques. Nano biotechnology, as a promising technology is also progressing, classically for cancer targeted drug development.
Bioprocess Engineering - INFLIBNET Centre
Bioprocess engineering encompasses the design, operation, control, and optimization of biochemical processes involving various biological pathways or reactions mediated by living cells …
Cell Culture Bioprocess Engineering - cms.sunhealth.org
Jan 7, 2025 · Bioprocess Engineering presents the design, fabrication, and control of a new type of bioreactor meant especially for animal cell line culture. The new bioreactor, called the see-saw …
Cell Culture Bioprocess Engineering (Download Only)
synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production In addition the book discusses the most …
CHEE 512. STEM CELL BIOPROCESS ENGINEERING.
Introduction to stem cell biology. Cell growth models applicable to stem cells and their progeny. Upstream processing (cell culture systems, bioreactors), downstream processing (cell …
Bioprocessing Basics - Eppendorf
Broadly speaking, bioreactors and fermenters are culture systems to produce cells or organisms. They are used in various applications, including basic research and development, and the …
Chapter 5 Bioreactor Design and Scale-Up - Springer
Design and selection of cell culture bioreactors are affected by cell-specific demands, engineering aspects, as well as economic and regulatory considerations. Mainly special demands, such as …
Unlocking the potential of cultivated meat through cell line …
Cell line engineering can be used to confer a diverse array of phenotypes in mammalian cells relevant for industrial-scale cultivated meat bioprocesses. These phenotypes can not only …
Cell Culture Bioprocess Engineering - openedconsortium.org
Bioprocess Engineering presents the design, fabrication, and control of a new type of bioreactor meant especially for animal cell line culture. The new bioreactor, called the see-saw bioreactor, is …
BIOREACTORS AND CULTIVATION SYSTEMS FOR CELL …
Regenerative medicine using human primary and stem cells opens new therapeutic concepts. Additionally, plant cell and tissue cultures for the production of biologically active substances …
Cell Culture: An Invaluable Technique for Biomedical Research
Cell culture is an indispensable technique in biomedical research, enabling scientists to explore the intricacies of cell biology, investigate disease mechanisms, and develop innovative therapies.
Bioprocess Engineering: Basic Concepts - Pearson …
1 WHAT IS A BIOPROCESS ENGINEER? 1.1. Biotechnology and Bioprocess Engineering. 1.2. Differing Approaches to Research for Biologists and Engineers. 1.3. The Story of Penicillin: How Biologists …
Cell Culture Bioprocess Engineering (2024)
Cell Culture Bioprocess Engineering Wei-Shou Hu,2020 This reference guide is designed for industrial practitioners of mammalian cell cultivation for the production of biologics. This work is …
Cell Culture Bioprocess Engineering (book) - old.icapgen.org
principles and essential knowledge of cell culture processes from across many different disciplines and presents them in a series of easy to follow comprehensive chapters Practicality including …
Bio Process Engineering Principles - vols.wta.org
It distills the fundamental principles and essential knowledge of cell culture processes from across many different disciplines and presents them in a series of easy-to-follow, comprehensive …
