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| debris removal solution miltenyi: Understanding the Roles of Glia and Circulating Leukocytes in Neurodegenerative Diseases Tuan Leng Tay, Giuseppe Locatelli, Gabriela Constantin, V. Wee Yong, 2022-05-03 |
| debris removal solution miltenyi: Tumor Immunology and Immunotherapy - Integrated Methods Part A , 2020-02-28 Tumor Immunology and Immunotherapy Integrated Methods - Part A, Volume 635 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Specific chapters to this release include Deconvolution of the immunological contexture of mouse tumors with multiplexed immunohistochemistry, High-dimensional multiplexed immunohistochemical characterization of immune contexture in human cancers, Multiplex assay by IHC for melanoma tumor microenvironment evaluation, Characterization of the tumor immune microenvironment by multispectral image analysis of multiplex immunofluorescence images, Phenotyping of immune cells in situ using multispectral imaging quantification, and much more. - Authored by leaders in the field of enzymology - Provides a comprehensiveness level of discussion on the field - Presents a highly specialized group of topics that delve deep into new updates and future prospects |
| debris removal solution miltenyi: Tissue-Resident Macrophages Elvira Mass, 2023-08-28 This detailed book delves into the diverse techniques and applications to target, isolate, image, phenotype, and analyze tissue-resident and monocyte-derived macrophages. The contents aim to describe the current knowledge about macrophage development and function which forces the scientific field to move beyond the previously described M1/M2 macrophage paradigm to be able to dissect macrophage functions within their specific niches during health and disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Tissue-Resident Macrophages: Methods and Protocols provides scientists entering the macrophage field with information and tools that allow them to dive into the state-of-the-art methodology used in this vital field. |
| debris removal solution miltenyi: The role of monocytes/macrophages in autoimmunity and autoinflammation Atsushi Kawakami, Keishi Fujio, Naoki Iwamoto, 2022-12-22 |
| debris removal solution miltenyi: Recombinant Protein Expression in Mammalian Cells David L. Hacker, |
| debris removal solution miltenyi: Glial Dysfunction in Epileptogenesis Kjell Heuser, Marco De Curtis, Christian Steinhaeuser, 2021-09-14 |
| debris removal solution miltenyi: Neural control of immunity Maureen Ann Cox, Peder S. Olofsson, Michael D. Burton, 2023-03-24 |
| debris removal solution miltenyi: Neural Progenitor Cells Loic P. Deleyrolle, 2021-09-24 This updated edition collects cutting-edge techniques used to study neural stem and progenitor cells as well as the brain microenvironment. Featuring a wide range of technological advances in the study of neural stem cells, the volume highlights the promises of stem cell-based therapeutic applications for central nervous system ailments. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neural Progenitor Cells: Methods and Protocols, Second Edition serves as an invaluable resource for the next generation of neuroscientists as they develop innovative experimental paradigms and progress toward therapeutic applications in the field of neurobiology. |
| debris removal solution miltenyi: Pluripotent Cells for Stroke: from Mechanism to Therapeutic Strategies Yujie Chen, Yao Yao, John Zhang, Shilei Hao, 2021-09-24 |
| debris removal solution miltenyi: Molecules of the Extracellular Matrix as Cancer Targets Nikos Karamanos, Martin Götte, Alberto Passi, 2022-11-14 |
| debris removal solution miltenyi: Crosstalk between Peripheral and Local Immune Response in the Pathophysiology of Stroke and Neurodegeneration Diseases, Volume II Yuanjian Fang, Lei Huang, Xiangsheng Zhang, Anwen Shao, Dirk M. Hermann, 2024-03-06 Accumulating evidence reveals both local and peripheral immune systems participated in the pathophysiology changes of acute and chronic neurological diseases. Immune cell activation facilitates inflammatory response in neurological diseases such as stroke, Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis. The immune response initiated by brain local cells (microglia and astrocytes) and peripheral blood cells (monocytes/macrophages, neutrophil, T cells, B cells), are now commonly thought to contribute “double-edged sword” effects to the progression of neurological diseases, which not only promoting repair and recovery, but also accelerating brain injury. Meanwhile, local and peripheral immune responses have complex crosstalk in the development of post-stroke injury and neurodegeneration disease. |
| debris removal solution miltenyi: Molecular Signalling and Pathways Editor’s Picks 2021 Jean-Marc Taymans, 2021-07-29 |
| debris removal solution miltenyi: Myc as a Disease Target Beyond Cancer Jonathan R. Whitfield, Mariano F. Zacarias-Fluck, John Sedivy, Yulia A. Nevzorova, 2024-09-02 Myc is a pleiotropic transcription factor fundamental for many cellular processes. It is an infamous and highly-studied oncogene, with around 10,000 articles published in the last decade. The majority of these link it to cancer. The focus on Myc as a hot target in cancer has led to immense efforts – from academia through to pharmaceutical companies – being directed towards finding a Myc inhibitor. Not surprisingly, given its fundamental nature in many physiological processes, Myc has also been linked to a large variety of pathologies other than cancer. The growing efforts to develop Myc inhibitors as cancer therapies will have important implications for the treatment of these diseases too, whether they have a clearly-demonstrated aetiology linked with Myc, or so far are only at early stages of association with it. Interestingly, the activation of Myc may also prove helpful in the field of regenerative medicine and reversal of ageing. The aim of this Research Topic is therefore to describe non-oncological pathologies and conditions to which Myc modulation could be applied. These will include conditions that have strong links to Myc and where Myc modulation has already been modelled, as well as conditions that so far have more limited data linking them to Myc but where it seems to have a key role. Authors will be expected to briefly review the particular disease and what is known to link Myc to it, plus the possibility of any clinical trials in the near future |
| debris removal solution miltenyi: Flow Cytometry and Cell Sorting Andreas Radbruch, 2013-03-14 The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended. CYTOBIOS |
| debris removal solution miltenyi: Thermogenic Fat Irfan J. Lodhi, 2023-04-19 This volume presents a broad collection of state-of-the-art methods to study the biology of thermogenic fat using in vitro cell culture and animal models. Chapters guide the readers through protocols on differentiation of human pluripotent stem cells and murine adipocyte precursors; methods for measuring mitochondrial respiration, heat generation, brown fat activation, and effects on energy metabolism in mice; and techniques for AAV-mediated gene delivery, transplantation of adipose tissue, isolation of adipose tissue immune cells and extracellular vesicles, and mass spectrometry-based profiling of brown fat lipids. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Thermogenic Fat: Methods and Protocols aims to be comprehensive guide for researchers in the field. |
| debris removal solution miltenyi: Sample Preparation Techniques for Chemical Analysis Massoud Kaykhaii, 2021-12-22 Despite having powerful software, microchips, and solid-state detectors that enable analytical chemists to achieve fast, stable, and accurate signals from their instruments, sample preparation is the most important step in chemical analysis. Issues can arise at this step for various reasons, including a low concentration of analytes, incompatibility of the sample with the analytical instrument, and matrix interferences. This volume discusses the basics of sample preparation and examines modern techniques that can be used by both novice and expert analytical chemists. Chapters review microextraction, surface spectroscopy analysis, and techniques for particle, tissue, and cellular separation. |
| debris removal solution miltenyi: Stem Cell Bioprocessing Tiago G. Fernandes, M. Margardia Diogo, Joaquim M.S. Cabral, 2013-11-15 Stem cell bioprocessing describes the main large-scale bioprocessing strategies for both stem cell culture and purification, envisaging the application of these cells for regenerative medicine and drug screening. Bioreactor configurations are described, including their applications for stem cell expansion, and stem cell separation techniques such as isolation and purification are discussed. Basic definitions are provided concerning the different types of stem cells, from adult stem cells to the more recent induced pluripotent stem cells. The main characteristics of these different stem cell types are described, alongside the molecular mechanisms underlying their self-renewal and differentiation. The book also focuses on methodologies currently used for in vitro stem cell culture under static conditions, including the challenge of xeno-free culture conditions, as well as culture parameters that influence stem cell culture. Approaches for both stem cell culture and separation in micro-scale conditions are presented, including the use of cellular microarrays for high-throughput screening of the effect of both soluble and extracellular matrix molecules. A further section is dedicated to application of stem cells for regenerative medicine. - Maintains a unique focus on both the basic stem cell biology concepts, and their translation to large-scale bioprocessing approaches - Envisages the use of stem cells in regenerative medicine and drug screening applications - Discusses the application of microscale techniques as a tool to perform basic stem cell biology studies |
| debris removal solution miltenyi: Immunophenotyping J Philip McCoy Jr, 2019-09-29 This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field. |
| debris removal solution miltenyi: In Living Color Rochelle A. Diamond, Susan DeMaggio, 2012-12-06 A contribution towards making this increasingly valuable technology accessible to researchers, including the students, post-doctoral scholars, and technicians gathering the knowledge inherent in this integration between analysis and physical isolation/purification methodologies. A step-by-step approach to the methodology for measuring various attributes demonstrated in the particular cells of interest is provided, as is a myriad of resources to fuel the curiosity and answer questions of both new and adept users. This book stems from the editors'experiences managing flow cytometry/cell sorting core facilities for the emerging researchers, in particular in developmental, cellular, and molecular biology. |
| debris removal solution miltenyi: Basic Cell Culture Protocols Cheryl D. Helgason, Cindy L. Miller, 2012-11-20 At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular BiologyTM, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success. |
| debris removal solution miltenyi: Astrocytes Barbara Di Benedetto, 2019-01-08 This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains. Beginning with some overviews of the subject, the book continues by covering techniques for the isolation of astrocytes from animal models, the investigation of astrocyte morphology and function, as well as for understanding astrocyte pathologies in the central nervous system. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Astrocytes: Methods and Protocols serves as an ideal guide for both experienced and beginner scientists working toward unraveling the novel, fascinating roles of these versatile cells. |
| debris removal solution miltenyi: Angiogenesis Assays Carolyn A. Staton, Claire Lewis, Roy Bicknell, 2007-01-11 Angiogenesis, the development of new blood vessels from the existing vasculature, is essential for physiological growth and over 18,000 research articles have been published describing the role of angiogenesis in over 70 different diseases, including cancer, diabetic retinopathy, rheumatoid arthritis and psoriasis. One of the most important technical challenges in such studies has been finding suitable methods for assessing the effects of regulators of eh angiogenic response. While increasing numbers of angiogenesis assays are being described both in vitro and in vivo, it is often still necessary to use a combination of assays to identify the cellular and molecular events in angiogenesis and the full range of effects of a given test protein. Although the endothelial cell - its migration, proliferation, differentiation and structural rearrangement - is central to the angiogenic process, it is not the only cell type involved. the supporting cells, the extracellular matrix and the circulating blood with its cellular and humoral components also contribute. In this book, experts in the use of a diverse range of assays outline key components of these and give a critical appraisal of their strengths and weaknesses. Examples include assays for the proliferation, migration and differentiation of endothelial cells in vitro, vessel outgrowth from organ cultures, assessment of endothelial and mural cell interactions, and such in vivo assays as the chick chorioallantoic membrane, zebrafish, corneal, chamber and tumour angiogenesis models. These are followed by a critical analysis of the biological end-points currently being used in clinical trials to assess the clinical efficacy of anti-angiogenic drugs, which leads into a discussion of the direction future studies should take. This valuable book is of interest to research scientists currently working on angiogenesis in both the academic community and in the biotechnology and pharmaceutical industries. Relevant disciplines include cell and molecular biology, oncology, cardiovascular research, biotechnology, pharmacology, pathology and physiology. |
| debris removal solution miltenyi: Diagnostic Cytogenetics Rolf-Dieter Wegner, 2013-11-11 Following a section on tissue culture, chromosome staining and basic information about karyotyping, this text presents nomenclature and quality standards, as well as protocols of relevance to comprehensive cytogenetic diagnostics. |
| debris removal solution miltenyi: Cellular and Molecular Mechanisms Underlying the Pathogenesis of Hepatic Fibrosis Ralf Weiskirchen, 2020-12-29 Worldwide, liver fibrosis is a major cause of morbidity and mortality and is associated with a high medical and economic burden. It is the common consequence of chronic liver injury due to various etiologies. During fibrogenesis, there is a progressive substitution of the liver parenchyma by scar tissue. Recent advances in the understanding of the history of liver fibrosis have shown that the pathogenesis is driven by different cell types and a large variety of soluble mediators. At present, scientists working in this field aim to increase basic knowledge, improve diagnostics, and try to translate experimental findings into new treatment modalities. This book includes 12 selected contributions from the Special Issue “Cellular and Molecular Mechanisms Underlying the Pathogenesis of Hepatic Fibrosis” that was published in Cells. These articles summarize current perspectives and findings in hepatic fibrosis research showing how scientists try to use basic scientific research to create new therapies and diagnostics. |
| debris removal solution miltenyi: Precise Genome Editing Techniques and Applications Zhiying Zhang, David Jay Segal, Kun Xu, 2020-06-04 |
| debris removal solution miltenyi: Culture of Human Stem Cells R. Ian Freshney, Glyn N. Stacey, Jonathan M. Auerbach, 2007-07-16 This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study. |
| debris removal solution miltenyi: Mucosal Vaccines Hiroshi Kiyono, 1996-10-23 This comprehensive, authoritative treatise covers all aspects of mucosal vaccines including their development, mechanisms of action, molecular/cellular aspects, and practical applications. The contributing authors and editors of this one-of-a-kind book are very well known in their respective fields. Mucosal Vaccines is organized in a unique format in which basic, clinical, and practical aspects of the mucosal immune system for vaccine development are described and discussed. This project is endorsed by the Society for Mucosal Immunology. - Provides the latest views on mucosal vaccines - Applies basic principles to the development of new vaccines - Links basic, clinical, and practical aspects of mucosal vaccines to different infectious diseases - Unique and user-friendly organization |
| debris removal solution miltenyi: Machiavelli: The Prince Niccolo Machiavelli, 1988-10-28 Professor Skinner presents a lucid analysis of Machiavelli's text as a response to the world of Florentine politics. |
| debris removal solution miltenyi: Monoclonal Antibodies and Their Functional Fragments in Research, Diagnosis and Therapy Menotti Ruvo, 2022-02-11 |
| debris removal solution miltenyi: Natural Killer Cells Michael T. Lotze, Angus W. Thomson, 2009-11-12 Natural Killer Cells explains the importance of killer cells and how they are produced. It mentions that the most likely explanation for killer cell production is that they serve as a complementary system for T cells as a primary defense against viruses. However, these cells defend against certain viruses only, such as herpes viruses and influenza viruses. The book also explains the primary functions of killer cells, and it discusses how these cells help recognize damaged tissues, limit further damage to tissues, and regenerate damaged tissues. It discusses how these cells mature and develop, and it covers the different isolation, culture, and propagation methods of these cells. Furthermore, it focuses on the different killer cells that are present in various parts of the human body. The book concludes by explaining that natural killer cells are utilized for clinical therapy of malignancies, and that they have led to positive outcomes in the field of biology and medicine. - Provides a broad, detailed coverage of the biology and interactions of NK cells for students, fellows, scientists, and practitioners - Includes figures, histologic sections, and illustrations of the ontogeny of NK cells |
| debris removal solution miltenyi: Intestinal Stem Cell Niche , 2018-04-24 Advances in Stem Cells and Their Niches addresses stem cells during development, homeostasis, and disease/injury of the respective organs, presenting new developments in the field, including new data on disease and clinical applications. Video content illustrates such areas as protocols, transplantation techniques, and work with mice. Explores not only reviews of research, but also shares methods, protocols, and transplantation techniques Contains video content to illustrate such areas as protocols, transplantation techniques, and work with mice Each volume concentrates on one organ, making this a unique publication |
| debris removal solution miltenyi: Lung Innate Immunity and Inflammation Scott Alper, William J. Janssen, 2018-07-09 This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols aims to be a guidebook and will be of value and interest to researchers investigating innate immunity and inflammation in the lung as well as other organs and tissues. |
| debris removal solution miltenyi: Flow Cytometry Protocols Teresa S. Hawley, |
| debris removal solution miltenyi: Industrial Pharmaceutical Biotechnology Heinrich Klefenz, 2002-04-22 This volume focuses on pharmaceutical biotechnology as a key area of life sciences. The complete range of concepts, processes and technologies of biotechnology is applied in modern industrial pharmaceutical research, development and production. The results of genome sequencing and studies of biological-genetic function are combined with chemical, micro-electronic and microsystem technology to produce medical devices and diagnostic biochips. A multitude of biologically active molecules is expanded by additional novel structures created with newly arranged gene clusters and bio-catalytic chemical processes. New organisational structures in the co-operation of institutes, companies and networks enable faster knowledge and product development and immediate application of the results of research and process development. This book is the ideal source of information for scientists and engineers in research and development, for decision-makers in biotech, pharma and chemical corporations, as well as for research institutes, but also for founders of biotech companies and people working for venture capital corporations. |
| debris removal solution miltenyi: Microglia Bertrand Joseph, José Luis Venero, 2013-06-28 Key discoveries concerning the different biological functions of microglia in health and disease have attracted scientists from various fields. In Microglia: Methods and Protocols, expert researchers in the field detail methods for selection of the key cellular, molecular and biochemical techniques that are used in studying the many and varied functions of this fascinating cell. These methods and techniques include microglia cell culture for studying microglia activation and functions, as well as their interaction with other cell types both in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microglia: Methods and Protocols is a useful resource for cell biologists, molecular biologists, immunologists, oncologist and neuroscientists. |
| debris removal solution miltenyi: Introduction to Single Cell Omics Xinghua Pan, Shixiu Wu, Sherman M. Weissman, 2019-09-19 Single-cell omics is a progressing frontier that stems from the sequencing of the human genome and the development of omics technologies, particularly genomics, transcriptomics, epigenomics and proteomics, but the sensitivity is now improved to single-cell level. The new generation of methodologies, especially the next generation sequencing (NGS) technology, plays a leading role in genomics related fields; however, the conventional techniques of omics require number of cells to be large, usually on the order of millions of cells, which is hardly accessible in some cases. More importantly, harnessing the power of omics technologies and applying those at the single-cell level are crucial since every cell is specific and unique, and almost every cell population in every systems, derived in either vivo or in vitro, is heterogeneous. Deciphering the heterogeneity of the cell population hence becomes critical for recognizing the mechanism and significance of the system. However, without an extensive examination of individual cells, a massive analysis of cell population would only give an average output of the cells, but neglect the differences among cells. Single-cell omics seeks to study a number of individual cells in parallel for their different dimensions of molecular profile on genome-wide scale, providing unprecedented resolution for the interpretation of both the structure and function of an organ, tissue or other system, as well as the interaction (and communication) and dynamics of single cells or subpopulations of cells and their lineages. Importantly single-cell omics enables the identification of a minor subpopulation of cells that may play a critical role in biological process over a dominant subpolulation such as a cancer and a developing organ. It provides an ultra-sensitive tool for us to clarify specific molecular mechanisms and pathways and reveal the nature of cell heterogeneity. Besides, it also empowers the clinical investigation of patients when facing a very low quantity of cell available for analysis, such as noninvasive cancer screening with circulating tumor cells (CTC), noninvasive prenatal diagnostics (NIPD) and preimplantation genetic test (PGT) for in vitro fertilization. Single-cell omics greatly promotes the understanding of life at a more fundamental level, bring vast applications in medicine. Accordingly, single-cell omics is also called as single-cell analysis or single-cell biology. Within only a couple of years, single-cell omics, especially transcriptomic sequencing (scRNA-seq), whole genome and exome sequencing (scWGS, scWES), has become robust and broadly accessible. Besides the existing technologies, recently, multiplexing barcode design and combinatorial indexing technology, in combination with microfluidic platform exampled by Drop-seq, or even being independent of microfluidic platform but using a regular PCR-plate, enable us a greater capacity of single cell analysis, switching from one single cell to thousands of single cells in a single test. The unique molecular identifiers (UMIs) allow the amplification bias among the original molecules to be corrected faithfully, resulting in a reliable quantitative measurement of omics in single cells. Of late, a variety of single-cell epigenomics analyses are becoming sophisticated, particularly single cell chromatin accessibility (scATAC-seq) and CpG methylation profiling (scBS-seq, scRRBS-seq). High resolution single molecular Fluorescence in situ hybridization (smFISH) and its revolutionary versions (ex. seqFISH, MERFISH, and so on), in addition to the spatial transcriptome sequencing, make the native relationship of the individual cells of a tissue to be in 3D or 4D format visually and quantitatively clarified. On the other hand, CRISPR/cas9 editing-based In vivo lineage tracing methods enable dynamic profile of a whole developmental process to be accurately displayed. Multi-omics analysis facilitates the study of multi-dimensional regulation and relationship of different elements of the central dogma in a single cell, as well as permitting a clear dissection of the complicated omics heterogeneity of a system. Last but not the least, the technology, biological noise, sequence dropout, and batch effect bring a huge challenge to the bioinformatics of single cell omics. While significant progress in the data analysis has been made since then, revolutionary theory and algorithm logics for single cell omics are expected. Indeed, single-cell analysis exert considerable impacts on the fields of biological studies, particularly cancers, neuron and neural system, stem cells, embryo development and immune system; other than that, it also tremendously motivates pharmaceutic RD, clinical diagnosis and monitoring, as well as precision medicine. This book hereby summarizes the recent developments and general considerations of single-cell analysis, with a detailed presentation on selected technologies and applications. Starting with the experimental design on single-cell omics, the book then emphasizes the consideration on heterogeneity of cancer and other systems. It also gives an introduction of the basic methods and key facts for bioinformatics analysis. Secondary, this book provides a summary of two types of popular technologies, the fundamental tools on single-cell isolation, and the developments of single cell multi-omics, followed by descriptions of FISH technologies, though other popular technologies are not covered here due to the fact that they are intensively described here and there recently. Finally, the book illustrates an elastomer-based integrated fluidic circuit that allows a connection between single cell functional studies combining stimulation, response, imaging and measurement, and corresponding single cell sequencing. This is a model system for single cell functional genomics. In addition, it reports a pipeline for single-cell proteomics with an analysis of the early development of Xenopus embryo, a single-cell qRT-PCR application that defined the subpopulations related to cell cycling, and a new method for synergistic assembly of single cell genome with sequencing of amplification product by phi29 DNA polymerase. Due to the tremendous progresses of single-cell omics in recent years, the topics covered here are incomplete, but each individual topic is excellently addressed, significantly interesting and beneficial to scientists working in or affiliated with this field. |
| debris removal solution miltenyi: Lentivirus Gene Engineering Protocols Maurizio Federico, 2008-02-03 Cell gene engineering is emerging as a field with outstanding impact, not only in medicine/biology, but also, and perhaps most importantly, in agriculture and in all those food sciences involved in the fight against world hunger. Lentivirus vector-based technologies represent the last frontier in the development of powerful and reliable methods for both in vitro and in vivo gene transfer in eukaryotic animal cells. Although the design of lentivirus vectors is closely reminiscent of those already successfully applied to the construction of oncoretroviral vectors, some unique features, e.g., the ef- ciency in transducing both postmitotic and stem cells, render the use of lentivirus vectors invaluable. It has been a great pleasure to edit Lentivirus Gene Engineering Pro- cols, owing in part to the high level of enthusiasm that the authors dem- strated in contributing to this book. The fact that so many outstanding scientists engaged in lentivirus vector research have provided articles renders it so- thing more than a technical handbook. In addition to detailed descriptions of the most innovative methodologies, the reader may find very informative ov- views concerning both theoretical and practical aspects of the origin and the development of diverse lentivirus vector types. This, in my opinion, rep- sents a unique added value of this volume, which should help our work resist the passage of time, to which books such as this are particularly sensitive. |
| debris removal solution miltenyi: Cerebrovascular and Neurodegenerative Diseases – New Insights into Molecular Cell Biology and Therapeutic Targets Sikha Saha, Sriharsha Kantamneni, 2020-01-30 |
| debris removal solution miltenyi: Novel Approaches to Target the Immune System in Gastrointestinal Cancers Gianluigi Giannelli, Erica Villa, Maria L. Martinez Chantar, 2022-01-31 |
| debris removal solution miltenyi: Culturing Nerve Cells Gary Banker, Kimberly Goslin, 1998 A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. |
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Prepare 1× Binding Buffer by dilution of 20× Binding Buffer Stock Solution with sterile, double-distilled water. Collect cells, centrifuge at 300×g and remove supernatant completely. …
Single Cell 解析のためのサンプル調製 - Miltenyi Biotec
ミルテニーバイオテク社が提供するサンプルクリアリングのための製品ポートフォリオにより、サンプル中の不要物を除去することで最適な単細胞懸濁液を調製できます。 様々な組織から …
Debris Removal Solution - Miltenyi Biotec
The Debris Removal Solution has been developed for efficient removal of cell debris from all kind of tissue after dissociation. It has been particularly tested for debris removal from dissociated adult …
Miltenyi Biotec - GENOTOUL
• Debris & dead cells free Risks • Background noise in sequencing data • Impact on efficiency of cell partitioning • Impact on Cell Counting Accurancy
mouse and rat - Miltenyi Biotec
After dissociation, the Debris Removal Solution is used for the removal of debris followed by an subsequent removal of erythrocytes using the Red Blood Cell Removal Solution.
Increase Increase the the viability viability of of your your …
• Our Dead Cell Removal Kit achieves the efficient removal of dead cells from different types of samples, both by manual or automated separation using the autoMACS® Pro Separator. • …
Myelin Removal Beads II human, mouse, rat - Miltenyi Biotec
Myelin Removal Beads II allow for the specific removal of myelin debris from single-cell suspensions during sample preparation, leading to a higher purity and recovery of target cells, and thereby …
Removal of Dead Cells for Single Cell RNA Sequencing - 10x …
sample sizes compatible with Miltenyi Biotec LS columns (≤2×109 total cells), consult the manufacturer’s instructions. a. Centrifuge the cell sample at 300 rcf for 5 min (PBMCs) or 10 min …
Debris Removal Solution - 101.200.202.226
Cell debris often occurs after tissue dissociation and impairs downstream applications. The Debris Removal Solution has been developed for efficient removal of cell debris from all kind of tissue …
Cell preparation for genomics - Miltenyi Biotec
The Debris Removal Solution is a ready-to-use density-gradient reagent that allows the fast and convenient removal of debris in cell suspensions containing fragile cells, including cells from …
动物组织解离试剂盒 - oebiotech.com
本产品适用于人类、大鼠、小鼠等多种动物组织的细胞悬液制备。 经本公司长期验证,适用的组织类型包括:心脏、乳腺、生殖器官肿瘤(如卵巢癌、前列腺癌)、内脏肿瘤(如肺癌、胰腺癌、肾癌、肝癌等)、 …
Semi and fully automated immunostaining sample preparation …
With the application of a ready-to-use density gradient reagent, Debris Removal Solution (Miltenyi Biotec) improved the recovery to 18.6% viable CD45+ cells.
MACS® Sample Preparation - AdviSains
Innovative cell strainers and cleaning reagents help you to tune your sample for your downstream application by the removal of cell aggregates, debris, or other unwanted material, such as myelin, …
Tumor Dissociation Kit mouse - nwbiotec.com
14.(Optional) In case of excess of debris, use Debris Removal Solution (# 130-109-398). Refer to www.miltenyibiotec.com for all data sheets and protocols. Warranty The products sold …
10X Genomics Single Cell Sample Submission Guidelines
For larger debris, the MACS SmartFilter (Miltenyi Biotec) is recommended, for low cell suspension volumes, the Flowmi™ Cell Strainer (Merck) or the pluriStrainer® Mini (pluriSelect) are …
Chapter 25 Measuring the Metabolic State of Tissue-Resident …
70 μM strainer. To remove cell and myelin debris, centri-fuge cells at 300 g for 10 min at 4 °C, aspirate the supernatant, and resuspend pellet in 3100 μL ice-cold PBS. Transfer the cell …
Neuroscience - Azuleon
Myelin Removal Beads II have been developed for the specific removal of myelin debris from single-cell suspensions (tested for mouse, rat, human, and sheep samples), and are compatible with the …
10X GENOMICS SINGLE CELL GENE EXPRESSION
Debris: Tissue/cell debris in your suspension can cause clogging of the 10X microfluidic chip, contamination of your single cells with ambient RNA, and inaccurate cell counting.
Chapter 1 Characterization of Bone Marrow-Derived Immune
In this chapter, we outline an approach to characterize the immune compartment in murine brain metastatic tissue samples using spectral flow cytometry. Spectral flow cytometry is different …
CG000124 Rev E DEMONSTRATED PROTOCOL Nuclei …
Aggregate & Debris Removal • Filter lysed tissues and nuclei suspensions with an appropriate cell strainer to remove cellular debris and nuclei aggregates. • The MACS SmartStrainer is …
Dead Cell Removal Kit - Miltenyi Biotec
Prepare 1× Binding Buffer by dilution of 20× Binding Buffer Stock Solution with sterile, double-distilled water. Collect cells, centrifuge at 300×g and remove supernatant completely. …
Single Cell 解析のためのサンプル調製 - Miltenyi Biotec
ミルテニーバイオテク社が提供するサンプルクリアリングのための製品ポートフォリオにより、サンプル中の不要物を除去することで最適な単細胞懸濁液を調製できます。 様々な組織から分散した …
